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The map label for this gene is fixJ [H]

Identifier: 119776315

GI number: 119776315

Start: 3775147

End: 3775722

Strand: Direct

Name: fixJ [H]

Synonym: Sama_3183

Alternate gene names: 119776315

Gene position: 3775147-3775722 (Clockwise)

Preceding gene: 119776313

Following gene: 119776316

Centisome position: 87.67

GC content: 56.77

Gene sequence:

>576_bases
ATGCGCCTGTTTCTGGTGGACGATGATGAAGCCATTCGGGATTCACTGACCTATATGCTGACGCAGTGTGGTCGGCAGCT
AACCAGCTTTGCCAGTGCCGAAGCCTTTTTGGCGCAAGCAGACATCACCCTGCCCGGCTGCCTGATCCTCGACAGCCGTA
TGCCCGGCCTCAGCGGTGAGGCGCTGCAACATGAGCTGGTTAATCAGCAAAGTCCCCTTGGGATCATCTTTCTTACCGGC
CACGGCGACCTGCCCATGGCATTGGCAGCGTTTCGCGAAGGCGCCTGCGATTTCTTTCAAAAACCGGTTCAGGCCGCGCC
CTTGTGCGACGCTATCGATAAGGCTGAAGCTGAAAGTCAACGGCGCTACGAGGTTGCAGGGCTCAGGTTGAAACTTGCCC
GGCTCAGTGAACGGGAGTCCCAGGTGCTGCGGCTGTTGACCGATGGGCTAACCAACAAGCAAATTTCAGAAACCCTTTAC
CTGTCGCTCAGGACCATTGAAGTACACAGGGCCAGCATGATGAAAAAGCTTGGAGTACACAATCTGGCAGAGCTGGGGCG
TTTCGCCTGCCGCTGA

Upstream 100 bases:

>100_bases
AAAATACGCCGCCTGCGTGATCCATCGCCAAAAGCGAGCAAGCAAACTTGAGCCCTTCTCAACACTTTTTTAGGCTGGAA
GAAAAACGCGGGAGTAGCCT

Downstream 100 bases:

>100_bases
ATCAGGATGGGGGAAAATCGAGAGGTTCAGTGTCTGCCAGCCAACATAGGCTGGCAGAACGACCCGTTAGCCCTGTGTGG
AGAACAGAGACAACAGGAGC

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRLFLVDDDEAIRDSLTYMLTQCGRQLTSFASAEAFLAQADITLPGCLILDSRMPGLSGEALQHELVNQQSPLGIIFLTG
HGDLPMALAAFREGACDFFQKPVQAAPLCDAIDKAEAESQRRYEVAGLRLKLARLSERESQVLRLLTDGLTNKQISETLY
LSLRTIEVHRASMMKKLGVHNLAELGRFACR

Sequences:

>Translated_191_residues
MRLFLVDDDEAIRDSLTYMLTQCGRQLTSFASAEAFLAQADITLPGCLILDSRMPGLSGEALQHELVNQQSPLGIIFLTG
HGDLPMALAAFREGACDFFQKPVQAAPLCDAIDKAEAESQRRYEVAGLRLKLARLSERESQVLRLLTDGLTNKQISETLY
LSLRTIEVHRASMMKKLGVHNLAELGRFACR
>Mature_191_residues
MRLFLVDDDEAIRDSLTYMLTQCGRQLTSFASAEAFLAQADITLPGCLILDSRMPGLSGEALQHELVNQQSPLGIIFLTG
HGDLPMALAAFREGACDFFQKPVQAAPLCDAIDKAEAESQRRYEVAGLRLKLARLSERESQVLRLLTDGLTNKQISETLY
LSLRTIEVHRASMMKKLGVHNLAELGRFACR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=6e-14,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=29.4573643410853, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21167; Mature: 21167

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFLVDDDEAIRDSLTYMLTQCGRQLTSFASAEAFLAQADITLPGCLILDSRMPGLSGE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHH
ALQHELVNQQSPLGIIFLTGHGDLPMALAAFREGACDFFQKPVQAAPLCDAIDKAEAESQ
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHH
RRYEVAGLRLKLARLSERESQVLRLLTDGLTNKQISETLYLSLRTIEVHRASMMKKLGVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
NLAELGRFACR
HHHHHHHHHCC
>Mature Secondary Structure
MRLFLVDDDEAIRDSLTYMLTQCGRQLTSFASAEAFLAQADITLPGCLILDSRMPGLSGE
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHH
ALQHELVNQQSPLGIIFLTGHGDLPMALAAFREGACDFFQKPVQAAPLCDAIDKAEAESQ
HHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHH
RRYEVAGLRLKLARLSERESQVLRLLTDGLTNKQISETLYLSLRTIEVHRASMMKKLGVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
NLAELGRFACR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]