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The map label for this gene is metB [H]

Identifier: 119776303

GI number: 119776303

Start: 3759691

End: 3760851

Strand: Reverse

Name: metB [H]

Synonym: Sama_3171

Alternate gene names: 119776303

Gene position: 3760851-3759691 (Counterclockwise)

Preceding gene: 119776306

Following gene: 119776302

Centisome position: 87.34

GC content: 57.54

Gene sequence:

>1161_bases
ATGACACAGCGCAAAGCAGCCACCATCGCGGTGCGTCAGGGCATAGAGAGCGATACCCAGCATGGTGCCGTGGTTCCTCC
CATTTATCTGTCGACCAATTACTCCTTCGATGGCCACAAGAATCCGCGCAAATTTGACTATAGCCGCTCGGGTAACCCGA
CCCGCTGTATTCTGGGCGAGGCGTTGGCCGAGCTGGAGCAGGGTGCGACCGGCATTATTACATGCACAGGTATGGCTGCC
ATCACGTTGGTGACCAGCCTGCTGGGACCGGACGATCTTCTGGTAGTACCCCACGATTGTTATGGCGGCAGCTATCGTCT
CTTTACCAACCTCGCCAAAAAAGGTGCCTTTAAGCTGGCTGTGGTCGACCAGACAGATGATGCAGCGCTGGCGGCGGTAA
TTGCCCAAAAGCCACGCATGGTGTGGCTTGAAACCCCATCCAACCCGCTGCTGCGGGTAGTGGACATTCAGGCCATCTCT
GACGCCAGTCATGAAGTTGGCGCTCTGGTGGTAGTGGACAACACCTTCCTGTCGCCCATCTTGCAGCAGCCACTGCTTTT
GGGGGCCGACATAGTGATCCACTCCACCACCAAATACATTAACGGTCACAGCGATGTGGTCGGCGGCGCCGTGATTGCCA
GGGATGCCGAGCTCGGTGAAACCCTGCACTGGTGGTCCAATACTCTGGGACTGACCGGCAGCGCCTTTGACAGCTATCTT
ACCCTTCGTGGTATCCGTACCCTGGCTGTGCGGGTACGTGAGCATCAATCCAATGCGCAGCGCATAGTGGACGTGCTGAA
GAACAGCCCCCAGGTGAGCAAGGTGTATTACCCCGGTCTTGCAGACCACCCAGGCCATGACATAGCCGCACGGCAACAAA
AAGGCTTTGGCGCTATGCTGAGTTTTGAACTGGTGGGCGGTGAAAAAGAACTGGTGGCTTTCCTCGACGCATTACAGCAC
TTCAGTGTGGCCGAAAGCCTGGGAGGTGTTGAGTCGCTGGTTGCCGTGCCCGCTACCATGACCCACAGAGCCATGGTGCC
AGAAGCCCGCGCCGAGGCCGGAATTAAAGACACCCTGCTGCGACTGTCGGTGGGGATTGAAGATGCAGAAGATTTAGTGG
CCGATATCCAAGCCGGCCTGGCTGCCGTGGCAGCCTGTTGA

Upstream 100 bases:

>100_bases
TCTAAACGTCTTTATGCCTATTGACCTGTTGCCGGTGCTTAGGTAAATTTAGCCATCCAGACATCTTTATTTCCGTGCGG
TAACATCGGAGGAGTACAAA

Downstream 100 bases:

>100_bases
ATAGGAGTCAGTGATGGCGCGTTGTCATTTGCATAAGTTTGGTGGCTCCAGTTTGGCGGATGCCGATTGCTACCGCCGCG
TGGCCCATATCCTGTTGACC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTQRKAATIAVRQGIESDTQHGAVVPPIYLSTNYSFDGHKNPRKFDYSRSGNPTRCILGEALAELEQGATGIITCTGMAA
ITLVTSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGAFKLAVVDQTDDAALAAVIAQKPRMVWLETPSNPLLRVVDIQAIS
DASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVIARDAELGETLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRVREHQSNAQRIVDVLKNSPQVSKVYYPGLADHPGHDIAARQQKGFGAMLSFELVGGEKELVAFLDALQH
FSVAESLGGVESLVAVPATMTHRAMVPEARAEAGIKDTLLRLSVGIEDAEDLVADIQAGLAAVAAC

Sequences:

>Translated_386_residues
MTQRKAATIAVRQGIESDTQHGAVVPPIYLSTNYSFDGHKNPRKFDYSRSGNPTRCILGEALAELEQGATGIITCTGMAA
ITLVTSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGAFKLAVVDQTDDAALAAVIAQKPRMVWLETPSNPLLRVVDIQAIS
DASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVIARDAELGETLHWWSNTLGLTGSAFDSYL
TLRGIRTLAVRVREHQSNAQRIVDVLKNSPQVSKVYYPGLADHPGHDIAARQQKGFGAMLSFELVGGEKELVAFLDALQH
FSVAESLGGVESLVAVPATMTHRAMVPEARAEAGIKDTLLRLSVGIEDAEDLVADIQAGLAAVAAC
>Mature_385_residues
TQRKAATIAVRQGIESDTQHGAVVPPIYLSTNYSFDGHKNPRKFDYSRSGNPTRCILGEALAELEQGATGIITCTGMAAI
TLVTSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGAFKLAVVDQTDDAALAAVIAQKPRMVWLETPSNPLLRVVDIQAISD
ASHEVGALVVVDNTFLSPILQQPLLLGADIVIHSTTKYINGHSDVVGGAVIARDAELGETLHWWSNTLGLTGSAFDSYLT
LRGIRTLAVRVREHQSNAQRIVDVLKNSPQVSKVYYPGLADHPGHDIAARQQKGFGAMLSFELVGGEKELVAFLDALQHF
SVAESLGGVESLVAVPATMTHRAMVPEARAEAGIKDTLLRLSVGIEDAEDLVADIQAGLAAVAAC

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=344, Percent_Identity=43.0232558139535, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI299473759, Length=344, Percent_Identity=40.6976744186046, Blast_Score=248, Evalue=6e-66,
Organism=Homo sapiens, GI25453487, Length=343, Percent_Identity=37.3177842565598, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=61.5789473684211, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=29.7229219143577, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=347, Percent_Identity=44.956772334294, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=347, Percent_Identity=44.6685878962536, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.8648648648649, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=41.8181818181818, Blast_Score=280, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6321254, Length=407, Percent_Identity=35.1351351351351, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=31.5668202764977, Blast_Score=159, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=392, Percent_Identity=28.3163265306122, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=323, Percent_Identity=26.9349845201238, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6322970, Length=271, Percent_Identity=25.830258302583, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322590, Length=277, Percent_Identity=25.6317689530686, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323557, Length=249, Percent_Identity=27.3092369477912, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922554, Length=377, Percent_Identity=41.1140583554377, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41165; Mature: 41034

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRKAATIAVRQGIESDTQHGAVVPPIYLSTNYSFDGHKNPRKFDYSRSGNPTRCILGE
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALAELEQGATGIITCTGMAAITLVTSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGAFKLA
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEE
VVDQTDDAALAAVIAQKPRMVWLETPSNPLLRVVDIQAISDASHEVGALVVVDNTFLSPI
EEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVIARDAELGETLHWWSNTLGLTGSAFDSYL
HHCCHHHCCEEEEECCHHHCCCCCCHHCCEEEEECHHHCHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRVREHQSNAQRIVDVLKNSPQVSKVYYPGLADHPGHDIAARQQKGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEE
SFELVGGEKELVAFLDALQHFSVAESLGGVESLVAVPATMTHRAMVPEARAEAGIKDTLL
EEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCHHHHHCCHHHHHH
RLSVGIEDAEDLVADIQAGLAAVAAC
HHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQRKAATIAVRQGIESDTQHGAVVPPIYLSTNYSFDGHKNPRKFDYSRSGNPTRCILGE
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALAELEQGATGIITCTGMAAITLVTSLLGPDDLLVVPHDCYGGSYRLFTNLAKKGAFKLA
HHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCEEEE
VVDQTDDAALAAVIAQKPRMVWLETPSNPLLRVVDIQAISDASHEVGALVVVDNTFLSPI
EEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCEEEEEECCHHHHHH
LQQPLLLGADIVIHSTTKYINGHSDVVGGAVIARDAELGETLHWWSNTLGLTGSAFDSYL
HHCCHHHCCEEEEECCHHHCCCCCCHHCCEEEEECHHHCHHHHHHHHCCCCCCHHHHHHH
TLRGIRTLAVRVREHQSNAQRIVDVLKNSPQVSKVYYPGLADHPGHDIAARQQKGFGAML
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHCCCCCEEE
SFELVGGEKELVAFLDALQHFSVAESLGGVESLVAVPATMTHRAMVPEARAEAGIKDTLL
EEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHCCCHHHHHCCHHHHHH
RLSVGIEDAEDLVADIQAGLAAVAAC
HHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]