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The map label for this gene is rlmB [H]

Identifier: 119776198

GI number: 119776198

Start: 3643340

End: 3644062

Strand: Reverse

Name: rlmB [H]

Synonym: Sama_3066

Alternate gene names: 119776198

Gene position: 3644062-3643340 (Counterclockwise)

Preceding gene: 119776199

Following gene: 119776196

Centisome position: 84.62

GC content: 57.68

Gene sequence:

>723_bases
GTGTTTGGCATTCATGCGGTCGAGTCTGTGCTAAAGCATGCCCCCGAGCGCGTGCTCGAGTTGTGGCTGATGACCGGCCG
TCAGGACGAACGGGTGCTGCCACTCTTGGAAATAGCGCAAAACTGGGGCATCAAGGCCCAGCAGGCAGCACGCAAAACCC
TTGACGAAAAAGCCGAAAGCACACAGCATCAGGGCATTATCATTCGGGTGCGTCCGGCTAAGGTGTTGACTGAAAACGAT
CTCGAAGCTTTGCTCGATGCCACCGAAACCCCGTTTTTACTGATCCTCGATGGGGTGACAGACCCTCACAATCTGGGCGC
GTGTTTGCGTAATGCCGATGCGGCTGGCGTGCATGGGGTGATAGTGCCTAAGGACAACTCAGCCGGTTTGACGCCAACCG
TGAGTAAAGTGGCCTGCGGCGCGGCGGAAACCGTGCCTCTGTTTCAAGTGACCAACCTTGCCCGCACCATGAAGCGCATT
CAGGAGCGCGGCGTGTGGATTGCCGGTACCGCCGGTGAAGCTGACTCGACTCTGTATGAGGCCAATCTCAAAGGGCCGCT
TGCCATTGCCATGGGCGCGGAAGGCAAGGGCCTCAGACGCTTAAGCCGTGAAGGTTGCGATACCCTGATTTCCATCCCTA
TGGCAGGCAGTGTTTCCAGCCTCAACGTCTCTGTGGCGACCGGAATCTGTTTGTTTGAGGCCGTGCGTCAGCGGTTGGGA
TAA

Upstream 100 bases:

>100_bases
CAAGGCCAAAGACGGTAAGAAACCCAAAAAGGCGGCAGAAGCCAAGCCCAAGGCCGCTAAACGAGTTAAGAGAAAGAAAT
AAATGAAAAAACAGGAATTG

Downstream 100 bases:

>100_bases
CCTTCCCGCCGCAAGGTGTTTATCAAATAAAAAAGGATTGCCCAGGCAATCCTTTTTTATTGATGGAAACTGTGGGTGAT
TATTGCTTACTGGAAGGTAT

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MFGIHAVESVLKHAPERVLELWLMTGRQDERVLPLLEIAQNWGIKAQQAARKTLDEKAESTQHQGIIIRVRPAKVLTEND
LEALLDATETPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSAGLTPTVSKVACGAAETVPLFQVTNLARTMKRI
QERGVWIAGTAGEADSTLYEANLKGPLAIAMGAEGKGLRRLSREGCDTLISIPMAGSVSSLNVSVATGICLFEAVRQRLG

Sequences:

>Translated_240_residues
MFGIHAVESVLKHAPERVLELWLMTGRQDERVLPLLEIAQNWGIKAQQAARKTLDEKAESTQHQGIIIRVRPAKVLTEND
LEALLDATETPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSAGLTPTVSKVACGAAETVPLFQVTNLARTMKRI
QERGVWIAGTAGEADSTLYEANLKGPLAIAMGAEGKGLRRLSREGCDTLISIPMAGSVSSLNVSVATGICLFEAVRQRLG
>Mature_240_residues
MFGIHAVESVLKHAPERVLELWLMTGRQDERVLPLLEIAQNWGIKAQQAARKTLDEKAESTQHQGIIIRVRPAKVLTEND
LEALLDATETPFLLILDGVTDPHNLGACLRNADAAGVHGVIVPKDNSAGLTPTVSKVACGAAETVPLFQVTNLARTMKRI
QERGVWIAGTAGEADSTLYEANLKGPLAIAMGAEGKGLRRLSREGCDTLISIPMAGSVSSLNVSVATGICLFEAVRQRLG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=259, Percent_Identity=31.2741312741313, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1790623, Length=238, Percent_Identity=65.1260504201681, Blast_Score=328, Evalue=1e-91,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=27.0491803278689, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790083, Length=156, Percent_Identity=31.4102564102564, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=251, Percent_Identity=29.0836653386454, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=170, Percent_Identity=33.5294117647059, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25675; Mature: 25675

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGIHAVESVLKHAPERVLELWLMTGRQDERVLPLLEIAQNWGIKAQQAARKTLDEKAES
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TQHQGIIIRVRPAKVLTENDLEALLDATETPFLLILDGVTDPHNLGACLRNADAAGVHGV
HHHCCEEEEEECCHHHCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEE
IVPKDNSAGLTPTVSKVACGAAETVPLFQVTNLARTMKRIQERGVWIAGTAGEADSTLYE
EEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
ANLKGPLAIAMGAEGKGLRRLSREGCDTLISIPMAGSVSSLNVSVATGICLFEAVRQRLG
CCCCCCEEEEECCCCHHHHHHHHHCCHHEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGIHAVESVLKHAPERVLELWLMTGRQDERVLPLLEIAQNWGIKAQQAARKTLDEKAES
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TQHQGIIIRVRPAKVLTENDLEALLDATETPFLLILDGVTDPHNLGACLRNADAAGVHGV
HHHCCEEEEEECCHHHCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCEEE
IVPKDNSAGLTPTVSKVACGAAETVPLFQVTNLARTMKRIQERGVWIAGTAGEADSTLYE
EEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCEEEE
ANLKGPLAIAMGAEGKGLRRLSREGCDTLISIPMAGSVSSLNVSVATGICLFEAVRQRLG
CCCCCCEEEEECCCCHHHHHHHHHCCHHEEECCCCCCCCCCEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]