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The map label for this gene is ygfZ [C]

Identifier: 119776082

GI number: 119776082

Start: 3507032

End: 3507997

Strand: Direct

Name: ygfZ [C]

Synonym: Sama_2950

Alternate gene names: 119776082

Gene position: 3507032-3507997 (Clockwise)

Preceding gene: 119776081

Following gene: 119776086

Centisome position: 81.44

GC content: 58.39

Gene sequence:

>966_bases
ATGACATTTACCGTCTCACAGGCCGCCTGGGCGCTGGACAGCGAAGGCCCGGCATTGACCCTGAGCTTACTCAATCACCT
CGGGCTGGTGTCTGTCACCGGCGAGCAGGGCAACAGCTTTATCCACGGCCAGGTCACCGCCGATATCAGCGCACTGGAAC
CCGGCCAATGGCGCTGGGGTGCCCACTGCGATCCCAAGGGCCGTATGCTGGCGACCTTCCGTACCTTTAAACTGGGTGAC
GCCCTGATGATGCTGGAGCCGCGCAGCGCGCTTGAAGTCTCACTGGCACAGCTGAAAAAGTATGCCGTATTCAGCAAGGC
GGAACTTGTGGATATGAGTAGCGAACTGACCCTGCTGGGTGTCTCAGGTCCAGAGGCCGCCGGGTTTGTTGCCCGGCACT
TTGGCACAGCCGAGGCAGATGTTTTCAGCACTGAGCAAGGCACAGTGCTCAAAGACGGTGAGCGTTTTATCCTGATCCTG
GACAAGGCCAATGCCTGTGCCCTGATTGAGAAAAGTGGCCAGCCTCTCTATGGCGCTGGTGTGTGGCAAGCATTGGAAAT
CCGTGCCGGCTACCCCAATATCGCGGCCGGTCATGCCAGTGAGTACATTCCGCAGATGTGTAATCTGCAGGCCCTCGGCG
GAATAAGCTTCAACAAGGGCTGCTACATGGGTCAGGAAACCGTGGCCCGCACAAAATACCGTGGCGGCAACAAACGCGCG
CTCTACATCCTCTTTGGTGAAAGCCAGACACCGGTGCGTCTCGAAAGTACGCTGGAAATCGCCGTGGAAGGCGGGTATCG
CAAGGCGGGCAACATCATTGAGGTGGTTAGCCGTGGCAATAAGGTTCTGATGACCGCTGTCCTCGCCAATGACACAGCGC
CCGATGCGGTGCTGCGTCTCGCGGATGACCACGAAGCCAGTTTTACCCTGCTGCCTCTGCCCTACTCGCTGGAAGAAGAA
GCGTAA

Upstream 100 bases:

>100_bases
CTTTTTATTTTTGCGTGTCAGGCCTGAATATGGTCATTCACCCGGCCATTGGGATCCGCTACAATCCCAGACTATTAAAG
TTCATTTCAAGGAACAGGCA

Downstream 100 bases:

>100_bases
GTCCTGTCGCCGATTAACAAAAAGAGGCGCCAGATGCGCCTCTTTTTTATTCCTATCCGGGGACAGGTAAAAGCAACACT
GCCAAGTGCCAGACTAGCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTFTVSQAAWALDSEGPALTLSLLNHLGLVSVTGEQGNSFIHGQVTADISALEPGQWRWGAHCDPKGRMLATFRTFKLGD
ALMMLEPRSALEVSLAQLKKYAVFSKAELVDMSSELTLLGVSGPEAAGFVARHFGTAEADVFSTEQGTVLKDGERFILIL
DKANACALIEKSGQPLYGAGVWQALEIRAGYPNIAAGHASEYIPQMCNLQALGGISFNKGCYMGQETVARTKYRGGNKRA
LYILFGESQTPVRLESTLEIAVEGGYRKAGNIIEVVSRGNKVLMTAVLANDTAPDAVLRLADDHEASFTLLPLPYSLEEE
A

Sequences:

>Translated_321_residues
MTFTVSQAAWALDSEGPALTLSLLNHLGLVSVTGEQGNSFIHGQVTADISALEPGQWRWGAHCDPKGRMLATFRTFKLGD
ALMMLEPRSALEVSLAQLKKYAVFSKAELVDMSSELTLLGVSGPEAAGFVARHFGTAEADVFSTEQGTVLKDGERFILIL
DKANACALIEKSGQPLYGAGVWQALEIRAGYPNIAAGHASEYIPQMCNLQALGGISFNKGCYMGQETVARTKYRGGNKRA
LYILFGESQTPVRLESTLEIAVEGGYRKAGNIIEVVSRGNKVLMTAVLANDTAPDAVLRLADDHEASFTLLPLPYSLEEE
A
>Mature_320_residues
TFTVSQAAWALDSEGPALTLSLLNHLGLVSVTGEQGNSFIHGQVTADISALEPGQWRWGAHCDPKGRMLATFRTFKLGDA
LMMLEPRSALEVSLAQLKKYAVFSKAELVDMSSELTLLGVSGPEAAGFVARHFGTAEADVFSTEQGTVLKDGERFILILD
KANACALIEKSGQPLYGAGVWQALEIRAGYPNIAAGHASEYIPQMCNLQALGGISFNKGCYMGQETVARTKYRGGNKRAL
YILFGESQTPVRLESTLEIAVEGGYRKAGNIIEVVSRGNKVLMTAVLANDTAPDAVLRLADDHEASFTLLPLPYSLEEEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=318, Percent_Identity=41.1949685534591, Blast_Score=212, Evalue=2e-56,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 34516; Mature: 34385

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTVSQAAWALDSEGPALTLSLLNHLGLVSVTGEQGNSFIHGQVTADISALEPGQWRWG
CEEEECCCEEEECCCCCEEEEEHHHHCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCCC
AHCDPKGRMLATFRTFKLGDALMMLEPRSALEVSLAQLKKYAVFSKAELVDMSSELTLLG
CCCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEE
VSGPEAAGFVARHFGTAEADVFSTEQGTVLKDGERFILILDKANACALIEKSGQPLYGAG
CCCCCHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEECCCEEEEEECCCCEEECCC
VWQALEIRAGYPNIAAGHASEYIPQMCNLQALGGISFNKGCYMGQETVARTKYRGGNKRA
CEEEEEEECCCCCCCCCCHHHHHHHHHCEEEECCCEECCCCCCCHHHHHHHHCCCCCCEE
LYILFGESQTPVRLESTLEIAVEGGYRKAGNIIEVVSRGNKVLMTAVLANDTAPDAVLRL
EEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCEEHHCCCEEEEEEEECCCCCCHHEEEE
ADDHEASFTLLPLPYSLEEEA
CCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
TFTVSQAAWALDSEGPALTLSLLNHLGLVSVTGEQGNSFIHGQVTADISALEPGQWRWG
EEEECCCEEEECCCCCEEEEEHHHHCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCCC
AHCDPKGRMLATFRTFKLGDALMMLEPRSALEVSLAQLKKYAVFSKAELVDMSSELTLLG
CCCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCHHEEECCCCEEEEE
VSGPEAAGFVARHFGTAEADVFSTEQGTVLKDGERFILILDKANACALIEKSGQPLYGAG
CCCCCHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEECCCEEEEEECCCCEEECCC
VWQALEIRAGYPNIAAGHASEYIPQMCNLQALGGISFNKGCYMGQETVARTKYRGGNKRA
CEEEEEEECCCCCCCCCCHHHHHHHHHCEEEECCCEECCCCCCCHHHHHHHHCCCCCCEE
LYILFGESQTPVRLESTLEIAVEGGYRKAGNIIEVVSRGNKVLMTAVLANDTAPDAVLRL
EEEEECCCCCCEEEEEEEEEEEECCCCCCCCCCEEHHCCCEEEEEEEECCCCCCHHEEEE
ADDHEASFTLLPLPYSLEEEA
CCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]