Click here to switch to the map view.

The map label for this gene is nrfD [H]

Identifier: 119776036

GI number: 119776036

Start: 3451314

End: 3452255

Strand: Direct

Name: nrfD [H]

Synonym: Sama_2904

Alternate gene names: 119776036

Gene position: 3451314-3452255 (Clockwise)

Preceding gene: 119776035

Following gene: 119776041

Centisome position: 80.15

GC content: 57.32

Gene sequence:

>942_bases
ATGAGCGCTTTTCATTTCGAAGGCCTGGTATGGCATTGGCCTATCGCCATTTACCTCTTCCTGGCGGGTCTGTCTGCTGG
GGCGGTATTTTTCGCCATCATGCTGAAACACTTTAAGTTATTCCATGAAGCCTGGCATTCGCCCTTCGTGCAGGCGGCGG
CCATCATCGCACCTGTCGCCGTATTTGGTGGTCTGGGGATCCTGGTATTTGACCTCACCCGGCCACTGGATTTCTGGAAG
ATCCTGGTGTTCTACAATACCAGCTCTGTGATGTCCATGGGGGTGCTGGTGCTGATGGCCTATCAGGTGGTGCTGTTTGC
CTGGATAGCATGCGTCTTCCATGTGCGCATCGGCCAGTTGCTGGGCAATCGCTTGGCCATCGTCCGGCAGCTGCTCAATT
GGCTGGTTCTGCAGGAAGCTGCTATCACCGGCATTCTGGTGATCCTCGCCATCTCCCTTGGGGCCTACACCGGCTTCCTG
CTGTCGGCGCTGATAGGCTTCCCGCTGCTCAATAACCCGGTGCTGCCATTGCTGTTCCTGATCTCCGGGCTCTCCTCCGG
CGCCGCCGCAACCCTGCTGGGTGGTGTGCTGCTTCGGGGCAACCCCAATGGGGTGGAAGTGCGTTTTATCCACGGCATCG
AAATTCCCCTCATCCTGGTGGAAATTGGCCTGCTGTTTACCTTCTTCGCCGGATTAATCCTCTCCGGCGGTCAGAGCCAG
GTTGCGGCGCTCAACGCACTGGGGTTTGGCTTCTGGGGGTGGATATTCTGGGCCGGCGTTATCGGCCTAGGTCTTACCCT
GCCACTGGCCTTCAATCTGTGGATGAAAGTCTCGGCGGATCGTAAGTTCGCCTATGTGGCGGTAACCGCCAGTTTCAGCC
TTTTGGGGGTGTTCTTGCTGCGCAACTTTATCCTCTATACCGGCCAAATGACGGGGACCTAG

Upstream 100 bases:

>100_bases
TCAAGTTGCTTAAAGCCAAGCCCAGCTACCGCGCCAAGGTGGACCTGGGGACCCGGCCCAAGCTGTTCCATATTCAAACC
ATAGATGGGGAGATAATGCT

Downstream 100 bases:

>100_bases
CCTTCAATGTCACTCATACCCGAGAATTGTCTGACACACGGATAACCAATGCACATTTGTTATTGAACGCCACCAAAACA
CCCCGTCTTCGACCCGGTGT

Product: formate-dependent nitrite reductase, nrfD protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSAFHFEGLVWHWPIAIYLFLAGLSAGAVFFAIMLKHFKLFHEAWHSPFVQAAAIIAPVAVFGGLGILVFDLTRPLDFWK
ILVFYNTSSVMSMGVLVLMAYQVVLFAWIACVFHVRIGQLLGNRLAIVRQLLNWLVLQEAAITGILVILAISLGAYTGFL
LSALIGFPLLNNPVLPLLFLISGLSSGAAATLLGGVLLRGNPNGVEVRFIHGIEIPLILVEIGLLFTFFAGLILSGGQSQ
VAALNALGFGFWGWIFWAGVIGLGLTLPLAFNLWMKVSADRKFAYVAVTASFSLLGVFLLRNFILYTGQMTGT

Sequences:

>Translated_313_residues
MSAFHFEGLVWHWPIAIYLFLAGLSAGAVFFAIMLKHFKLFHEAWHSPFVQAAAIIAPVAVFGGLGILVFDLTRPLDFWK
ILVFYNTSSVMSMGVLVLMAYQVVLFAWIACVFHVRIGQLLGNRLAIVRQLLNWLVLQEAAITGILVILAISLGAYTGFL
LSALIGFPLLNNPVLPLLFLISGLSSGAAATLLGGVLLRGNPNGVEVRFIHGIEIPLILVEIGLLFTFFAGLILSGGQSQ
VAALNALGFGFWGWIFWAGVIGLGLTLPLAFNLWMKVSADRKFAYVAVTASFSLLGVFLLRNFILYTGQMTGT
>Mature_312_residues
SAFHFEGLVWHWPIAIYLFLAGLSAGAVFFAIMLKHFKLFHEAWHSPFVQAAAIIAPVAVFGGLGILVFDLTRPLDFWKI
LVFYNTSSVMSMGVLVLMAYQVVLFAWIACVFHVRIGQLLGNRLAIVRQLLNWLVLQEAAITGILVILAISLGAYTGFLL
SALIGFPLLNNPVLPLLFLISGLSSGAAATLLGGVLLRGNPNGVEVRFIHGIEIPLILVEIGLLFTFFAGLILSGGQSQV
AALNALGFGFWGWIFWAGVIGLGLTLPLAFNLWMKVSADRKFAYVAVTASFSLLGVFLLRNFILYTGQMTGT

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=314, Percent_Identity=47.4522292993631, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 34003; Mature: 33872

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFHFEGLVWHWPIAIYLFLAGLSAGAVFFAIMLKHFKLFHEAWHSPFVQAAAIIAPVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VFGGLGILVFDLTRPLDFWKILVFYNTSSVMSMGVLVLMAYQVVLFAWIACVFHVRIGQL
HHCCHHHHHHHHHCCHHHHEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNRLAIVRQLLNWLVLQEAAITGILVILAISLGAYTGFLLSALIGFPLLNNPVLPLLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ISGLSSGAAATLLGGVLLRGNPNGVEVRFIHGIEIPLILVEIGLLFTFFAGLILSGGQSQ
HHCCCCCHHHHHHCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHH
VAALNALGFGFWGWIFWAGVIGLGLTLPLAFNLWMKVSADRKFAYVAVTASFSLLGVFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
RNFILYTGQMTGT
HHHHHHCCCCCCC
>Mature Secondary Structure 
SAFHFEGLVWHWPIAIYLFLAGLSAGAVFFAIMLKHFKLFHEAWHSPFVQAAAIIAPVA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VFGGLGILVFDLTRPLDFWKILVFYNTSSVMSMGVLVLMAYQVVLFAWIACVFHVRIGQL
HHCCHHHHHHHHHCCHHHHEEEHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGNRLAIVRQLLNWLVLQEAAITGILVILAISLGAYTGFLLSALIGFPLLNNPVLPLLFL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ISGLSSGAAATLLGGVLLRGNPNGVEVRFIHGIEIPLILVEIGLLFTFFAGLILSGGQSQ
HHCCCCCHHHHHHCCEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHH
VAALNALGFGFWGWIFWAGVIGLGLTLPLAFNLWMKVSADRKFAYVAVTASFSLLGVFLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEEEHHHHHHHHHHHH
RNFILYTGQMTGT
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]