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The map label for this gene is pntA [H]

Identifier: 119775972

GI number: 119775972

Start: 3379308

End: 3380834

Strand: Direct

Name: pntA [H]

Synonym: Sama_2840

Alternate gene names: 119775972

Gene position: 3379308-3380834 (Clockwise)

Preceding gene: 119775968

Following gene: 119775973

Centisome position: 78.48

GC content: 56.65

Gene sequence:

>1527_bases
ATGCAAATTGGAATTCCGAGAGAGAGTCTCGCCGGTGAAACCCGGGTCGCCGCGACCCCGGCTACGGTTGAGCAACTTAA
AAAACTCGGCTTTGAAGTGGCTATCGAATCTGGTGCAGGCACACTTGCCAGCTTCAGTGATGCCGCCTTTGAGGCGGTGG
GTGCCAGTGTAGTGCCCTCCGTATGGCAGGCCGACCTTATCTTTAAGGTCAATGCCCCAACCGAAGATGAAATAAAAGAA
ATCAAAGACGGCGCAACCTTGGTGAGTTTTATCTGGCCTGCTCAAAACCCTGAGCTGGTTGAGAAATTATCACAGCGCAA
TATCAACGTGATGGCGATGGACATGGTGCCGCGTATTTCCCGCGCCCAGTCGCTGGATGCCCTGTCATCCATGGCCAACA
TCGGCGGCTATCGTGCCGTGGTGGAAGCCGCCCACGAATTTGGCCGCTTCTTTACCGGCCAAATCACCGCTGCCGGTAAG
GTCCCCCCTGCCAAGGTGCTGGTGATTGGTGCCGGTGTGGCGGGTCTTGCTGCCATTGGTACTGCAGGCTCCCTCGGCGC
CGTGGTGCGCGCCTTCGATACCCGCCTCGAAGTGGCGGAGCAAATCGAGTCCATGGGTGGCCAATTCCTCAAACTCGACT
TCGGCGGCGAAGACGGCTCATCCTCCGACGGCTATGCCAAGGTGATGAGCGAAGAGTTTATCAAGGCCGAGATGGCCCTC
TTTGCCGAACAGGCCAAAGAAGTGGACATTATCGTCACCACCGCCCTTATTCCCGGCAAGCCAGCGCCACGTCTTATCAC
CAAGGAAATGGTGGATTCGATGAAGAGCGGCTCTGTGATTGTGGACATGGCAGCCGCCACCGGCGGTAACTGTGAATACA
CAGTCCCGGGCGAGCTTTTTGTCACCGAAAACGGCGTTAAGGTGATTGGTTACACCGATCTGCCTGGCCGTCTGCCAGCC
CAGTCCTCCCAGCTTTACGGCACCAACCTGGTGAATCTGATGAAGCTGATGTGCAAAGACAAAGACGGCAATGCCGTTAT
CGACTTTGACGATGTGGTGATGCGCAACATGACAGTGGTCAAAGCCGGTGAAGTCACCTTCCCGCCGCCAGCCATCTCTG
TGTCTGCTGCCCCAGCCAAGGCTGCCCCCAAGGTAGAGCTGAAAAAAGCCGAGCCCAAAGAGCCTTCCAAACTCAAGTAC
GTTCTGGGCGCTCTGGGTCTGATTGGTTTTGGTGCTGTAGCTGCGGTAGCGCCGCCAGAGTTCTTGTCCCACTTCACCGT
GTTCCTGCTCTCCTGTGTGGTGGGCTACTACGTGGTGTGGAACGTAACTCACGCGCTGCACACGCCGCTGATGTCGGTAA
CCAACGCCATCTCGGGCATTATCGTGGTGGGCGCACTGCTGCAAATTGGCAGCGGCTCGACGCTGGTCACTGTGCTGGCG
TTTATTGCCGTGCTCATTGCCAGTATCAACATCTTCGGCGGCTTTACCGTCACTCAGCGCATGCTGAAGATGTTCCGTAA
GGATTAA

Upstream 100 bases:

>100_bases
CTACAGACAGCCGGTTGCAAAACCGTCCAGCCTGTCCCTCCTGTTTCGCCCGACACAGGAAGCGGTGTGTTCACGTTTCA
ATAACCATCAGGGAATCGTC

Downstream 100 bases:

>100_bases
GGGGGAATCACTGTGTCTCAAGGACTGGTAACAGCAGCCTACATAGTAGCAGCGCTGTGTTTTATTTTGAGTCTCGCGGG
CCTGTCCCGTCAGGAGACCG

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVAIESGAGTLASFSDAAFEAVGASVVPSVWQADLIFKVNAPTEDEIKE
IKDGATLVSFIWPAQNPELVEKLSQRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHEFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAVVRAFDTRLEVAEQIESMGGQFLKLDFGGEDGSSSDGYAKVMSEEFIKAEMAL
FAEQAKEVDIIVTTALIPGKPAPRLITKEMVDSMKSGSVIVDMAAATGGNCEYTVPGELFVTENGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKDKDGNAVIDFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKAAPKVELKKAEPKEPSKLKY
VLGALGLIGFGAVAAVAPPEFLSHFTVFLLSCVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGSGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Sequences:

>Translated_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVAIESGAGTLASFSDAAFEAVGASVVPSVWQADLIFKVNAPTEDEIKE
IKDGATLVSFIWPAQNPELVEKLSQRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHEFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAVVRAFDTRLEVAEQIESMGGQFLKLDFGGEDGSSSDGYAKVMSEEFIKAEMAL
FAEQAKEVDIIVTTALIPGKPAPRLITKEMVDSMKSGSVIVDMAAATGGNCEYTVPGELFVTENGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKDKDGNAVIDFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKAAPKVELKKAEPKEPSKLKY
VLGALGLIGFGAVAAVAPPEFLSHFTVFLLSCVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGSGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD
>Mature_508_residues
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVAIESGAGTLASFSDAAFEAVGASVVPSVWQADLIFKVNAPTEDEIKE
IKDGATLVSFIWPAQNPELVEKLSQRNINVMAMDMVPRISRAQSLDALSSMANIGGYRAVVEAAHEFGRFFTGQITAAGK
VPPAKVLVIGAGVAGLAAIGTAGSLGAVVRAFDTRLEVAEQIESMGGQFLKLDFGGEDGSSSDGYAKVMSEEFIKAEMAL
FAEQAKEVDIIVTTALIPGKPAPRLITKEMVDSMKSGSVIVDMAAATGGNCEYTVPGELFVTENGVKVIGYTDLPGRLPA
QSSQLYGTNLVNLMKLMCKDKDGNAVIDFDDVVMRNMTVVKAGEVTFPPPAISVSAAPAKAAPKVELKKAEPKEPSKLKY
VLGALGLIGFGAVAAVAPPEFLSHFTVFLLSCVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGSGSTLVTVLA
FIAVLIASINIFGGFTVTQRMLKMFRKD

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=537, Percent_Identity=50.2793296089386, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI122939153, Length=537, Percent_Identity=50.2793296089386, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1787887, Length=513, Percent_Identity=75.6335282651072, Blast_Score=765, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=523, Percent_Identity=51.434034416826, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 53465; Mature: 53465

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVAIESGAGTLASFSDAAFEAVGASVVPS
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHH
VWQADLIFKVNAPTEDEIKEIKDGATLVSFIWPAQNPELVEKLSQRNINVMAMDMVPRIS
HHCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECHHHHHH
RAQSLDALSSMANIGGYRAVVEAAHEFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAVVRAFDTRLEVAEQIESMGGQFLKLDFGGEDGSSSDGYAKVMSEEFIKAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
FAEQAKEVDIIVTTALIPGKPAPRLITKEMVDSMKSGSVIVDMAAATGGNCEYTVPGELF
HHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEEE
VTENGVKVIGYTDLPGRLPAQSSQLYGTNLVNLMKLMCKDKDGNAVIDFDDVVMRNMTVV
EECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHCEEEE
KAGEVTFPPPAISVSAAPAKAAPKVELKKAEPKEPSKLKYVLGALGLIGFGAVAAVAPPE
EECCEECCCCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
FLSHFTVFLLSCVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGSGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIGIPRESLAGETRVAATPATVEQLKKLGFEVAIESGAGTLASFSDAAFEAVGASVVPS
CCCCCCHHHHCCCCEEECCHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHH
VWQADLIFKVNAPTEDEIKEIKDGATLVSFIWPAQNPELVEKLSQRNINVMAMDMVPRIS
HHCCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECHHHHHH
RAQSLDALSSMANIGGYRAVVEAAHEFGRFFTGQITAAGKVPPAKVLVIGAGVAGLAAIG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHHHC
TAGSLGAVVRAFDTRLEVAEQIESMGGQFLKLDFGGEDGSSSDGYAKVMSEEFIKAEMAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
FAEQAKEVDIIVTTALIPGKPAPRLITKEMVDSMKSGSVIVDMAAATGGNCEYTVPGELF
HHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCEEECCCEEE
VTENGVKVIGYTDLPGRLPAQSSQLYGTNLVNLMKLMCKDKDGNAVIDFDDVVMRNMTVV
EECCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEHHHHHHHCEEEE
KAGEVTFPPPAISVSAAPAKAAPKVELKKAEPKEPSKLKYVLGALGLIGFGAVAAVAPPE
EECCEECCCCCCEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHH
FLSHFTVFLLSCVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGSGSTLVTVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKD
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]