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The map label for this gene is yafC [H]

Identifier: 119775859

GI number: 119775859

Start: 3261614

End: 3262528

Strand: Direct

Name: yafC [H]

Synonym: Sama_2727

Alternate gene names: 119775859

Gene position: 3261614-3262528 (Clockwise)

Preceding gene: 119775858

Following gene: 119775860

Centisome position: 75.74

GC content: 54.1

Gene sequence:

>915_bases
GTGAACCTAGATGATGTAGCACTGTTTGTTCAGGTTGTTGAGTTAGGTAGCTTTACCAAAGCCGCTGAACGAAGCGGCCT
GCCAAAATCGACCGTCAGCCGCCGTATCCGAGATCTTGAGCAAAGTCTTCGCGCCCGCCTGTTGGAGCGTACTACCCGGC
ACCTACTGCTGACTGAGGTGGGAGAGGCTTTTTTGCTGCGAGCCCGAGCCATACTGGCGGCTGTAGAAGCAACCGAATTG
GAAGTGGCTGGCGCTCAGCAAACCTTTGAAGGCGAACTGACACTCTATGCCCCCGATTTTCTGATGGAATGGTGCTCTCC
CCAGATTGCACGCTTTTTTGAGCGTTATCCCGAACTCTCCCTGCGGGTGCATGACATAGGACAGACGTCATCGACACTGG
CAGACAAGCGCTTTGATTTGCTGCTGACCCTGGGCGCACTGCGAGATTCCTCCTTTATCGCACATCCCATGGCCAAGCTG
CAGTTTGATCTCTACGCCACACCGGAATATCTGCAACAGCACCCGCTTCCTGAACACGCCAAGGCCCTTGGGCAGAAGCA
CGCGCTGGCCATCATGGAACTGGCCTCCCTGGATTTGTGTTGGCCATTGGCACAAATCCCCCTGTTTCGCCGCCCCCAAT
TTTGCACCCAGTCCCCCTATCTGCTGCGTTCTTTAGTGTTGCAGCACCGAGTGATAGGTTATCTGCCAGTGGCCATGGCT
GGTGATTTGGTAGCCCAAGGAAAGTTGGTTCCCTTATACAACCGGACTGTGAGAACTGCTGAACAGCTTTACGGCGTGTA
TCATTCCCGCCGTTTTGTACCTGAAAAAGTCAAGCTGCTGATGGAAGACATTCGCGCGGGGCTGCCGGGCAGATTGCAGC
AGTTGGAAGCTGAACAAGTTGATTGTTCCAATTAA

Upstream 100 bases:

>100_bases
CATCGTCTCCCCCCTCGTAATTAAGGCAAGCAAAGCTGCGTGACACGCCACCCATGCAGACATAATAACCTGATTATTAT
AATTTTTTAAGGTCGAGCCC

Downstream 100 bases:

>100_bases
TGGAACAGTTCATTGCATCCAGGCGGATTTATCCCGCTGATGACGGTTTTTACAATGATGGCAGAATTTTTTGTCACCGG
AGTTACGTCTATGATGCCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNLDDVALFVQVVELGSFTKAAERSGLPKSTVSRRIRDLEQSLRARLLERTTRHLLLTEVGEAFLLRARAILAAVEATEL
EVAGAQQTFEGELTLYAPDFLMEWCSPQIARFFERYPELSLRVHDIGQTSSTLADKRFDLLLTLGALRDSSFIAHPMAKL
QFDLYATPEYLQQHPLPEHAKALGQKHALAIMELASLDLCWPLAQIPLFRRPQFCTQSPYLLRSLVLQHRVIGYLPVAMA
GDLVAQGKLVPLYNRTVRTAEQLYGVYHSRRFVPEKVKLLMEDIRAGLPGRLQQLEAEQVDCSN

Sequences:

>Translated_304_residues
MNLDDVALFVQVVELGSFTKAAERSGLPKSTVSRRIRDLEQSLRARLLERTTRHLLLTEVGEAFLLRARAILAAVEATEL
EVAGAQQTFEGELTLYAPDFLMEWCSPQIARFFERYPELSLRVHDIGQTSSTLADKRFDLLLTLGALRDSSFIAHPMAKL
QFDLYATPEYLQQHPLPEHAKALGQKHALAIMELASLDLCWPLAQIPLFRRPQFCTQSPYLLRSLVLQHRVIGYLPVAMA
GDLVAQGKLVPLYNRTVRTAEQLYGVYHSRRFVPEKVKLLMEDIRAGLPGRLQQLEAEQVDCSN
>Mature_304_residues
MNLDDVALFVQVVELGSFTKAAERSGLPKSTVSRRIRDLEQSLRARLLERTTRHLLLTEVGEAFLLRARAILAAVEATEL
EVAGAQQTFEGELTLYAPDFLMEWCSPQIARFFERYPELSLRVHDIGQTSSTLADKRFDLLLTLGALRDSSFIAHPMAKL
QFDLYATPEYLQQHPLPEHAKALGQKHALAIMELASLDLCWPLAQIPLFRRPQFCTQSPYLLRSLVLQHRVIGYLPVAMA
GDLVAQGKLVPLYNRTVRTAEQLYGVYHSRRFVPEKVKLLMEDIRAGLPGRLQQLEAEQVDCSN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=33.6842105263158, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789639, Length=286, Percent_Identity=25.1748251748252, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI145693193, Length=185, Percent_Identity=33.5135135135135, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081978, Length=192, Percent_Identity=31.7708333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789440, Length=203, Percent_Identity=29.064039408867, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788748, Length=153, Percent_Identity=31.3725490196078, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787128, Length=214, Percent_Identity=27.1028037383178, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789173, Length=270, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=20.066889632107, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=178, Percent_Identity=26.9662921348315, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787589, Length=190, Percent_Identity=26.8421052631579, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1788297, Length=169, Percent_Identity=27.810650887574, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI157672245, Length=186, Percent_Identity=27.4193548387097, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788706, Length=161, Percent_Identity=30.4347826086957, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34380; Mature: 34380

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDDVALFVQVVELGSFTKAAERSGLPKSTVSRRIRDLEQSLRARLLERTTRHLLLTEV
CCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEAFLLRARAILAAVEATELEVAGAQQTFEGELTLYAPDFLMEWCSPQIARFFERYPELS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCHHHHHHHHHCCCCE
LRVHDIGQTSSTLADKRFDLLLTLGALRDSSFIAHPMAKLQFDLYATPEYLQQHPLPEHA
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCHHHHHHCCCCHHH
KALGQKHALAIMELASLDLCWPLAQIPLFRRPQFCTQSPYLLRSLVLQHRVIGYLPVAMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GDLVAQGKLVPLYNRTVRTAEQLYGVYHSRRFVPEKVKLLMEDIRAGLPGRLQQLEAEQV
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
DCSN
CCCC
>Mature Secondary Structure
MNLDDVALFVQVVELGSFTKAAERSGLPKSTVSRRIRDLEQSLRARLLERTTRHLLLTEV
CCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEAFLLRARAILAAVEATELEVAGAQQTFEGELTLYAPDFLMEWCSPQIARFFERYPELS
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCHHHHHHHHHCCCCE
LRVHDIGQTSSTLADKRFDLLLTLGALRDSSFIAHPMAKLQFDLYATPEYLQQHPLPEHA
EEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEEECCCHHHHHHCCCCHHH
KALGQKHALAIMELASLDLCWPLAQIPLFRRPQFCTQSPYLLRSLVLQHRVIGYLPVAMA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GDLVAQGKLVPLYNRTVRTAEQLYGVYHSRRFVPEKVKLLMEDIRAGLPGRLQQLEAEQV
HHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHC
DCSN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]