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The map label for this gene is nirK [H]

Identifier: 119775813

GI number: 119775813

Start: 3210736

End: 3211917

Strand: Direct

Name: nirK [H]

Synonym: Sama_2681

Alternate gene names: 119775813

Gene position: 3210736-3211917 (Clockwise)

Preceding gene: 119775809

Following gene: 119775814

Centisome position: 74.56

GC content: 58.38

Gene sequence:

>1182_bases
ATGAAAAACGAACAGCAATCTATCGAACATGGCGTGGACACCAACCGCAGACAATTTCTGGGTGGATCTGTGGGTGCTGG
TCTGGTGGGACTGGGGCTCGCTTCCGGACTAATGAGCAGCCAGGCCAATGCCACCACCGCAGGTAACAAGGCCAAAAGCG
ATCGCATGGCCAACTACACCGCCGATCAACTGGAGCGCGTAAAGCAGGAGCTGGTGCCGCCGCCAATGGTACCCAAGCAT
GATCAGGTGGCCACGGGTAAACCCAAGGTCATTGAAGTCCGCCTCGAAGTAGAAGAAAAGCAAATTGAGCTGGAAAATGG
CGTGTTTGCCTGGGTGTGTGCCTTCAACGGCAGTGTGCCCGGGCCACTCATTGTGTGCCACCAGCACGATTACGTTGAGC
TGACGCTGGTTAACCCCAAGACCAATGTGCTGTCACACAACATCGACCTGCACTCAGCCACAGGCGCGCTGGGCGGCGGC
GAACTGACTCTGGTTGCCCCCGGGCAGGAAGTCACCTTCCGCTTCAAAGCCACCAAGGCAGGTACCTTTGTGTATCACTG
TGCCCCGGGCGGCATCATGATCCCCTGGCACGTAGCCATGGGGATGCACGGCGCCATCATGGTACTGCCAAGGGAAGGCC
TGAGCGACGGTGATGGCAACCCACTGCACTACGACCGCGCCTATTACATTGGCGAAGCCGACTTCTATGTGCCCAAAGAT
GCACAGGGCAACTACAAGCGTTACAACCAATTGATGGAGAGCATGGAAGAAGTGCTCACCACCATGAAGACACTGCTGCC
TTCCCACATAGTGTTCAACGGCGGGGTAGGTGCCCTCACCGGCAAGCACGCCATGCCTGCCAAGGTGGGTGAGTCAGTGC
TCTTTATCCACTCCCAGGCCAACCGTGACACCCGCCCTCACATCATCGGCGGCCATGGTGACTATGTGTGGGCCGCAGGC
TCCTTTGCCGACCGCCCACAACGGGATCTGGAAACCTGGATGGTGGCCGGTGGCAGCGCGGCAGCTGCACTCTACACCTT
CCGTCAGCCCGGGCTGCACGCCTACGTTAACCACAACCTGATTGAGGCCATCATGAAAGGGGCTGCCGCCCACATCAAGG
TAGACGGTGAGTGGGATGACAACCTGATGGTACAGGTGAGAAAGCCCAGCGCCATCGCATAA

Upstream 100 bases:

>100_bases
GCGATAATTTAGACACCAGAAAAGGATTCTCAACACCAAGCGCCTCCTCTCAAGGAGGCCATAAAAACCATCACGCGATA
TCACAACCATGGAGTTTGGC

Downstream 100 bases:

>100_bases
ATCGCTGCGCGTTAACTGAGTCAGAGTGCGCTGACTGAGCCACAGCGCACTCCCGGCTCAAAGATGTGAAATACCTGGGT
GCAAACGCCAAGATGTACGC

Product: nitrite reductase (NO-forming)

Products: NA

Alternate protein names: Cu-NIR [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKNEQQSIEHGVDTNRRQFLGGSVGAGLVGLGLASGLMSSQANATTAGNKAKSDRMANYTADQLERVKQELVPPPMVPKH
DQVATGKPKVIEVRLEVEEKQIELENGVFAWVCAFNGSVPGPLIVCHQHDYVELTLVNPKTNVLSHNIDLHSATGALGGG
ELTLVAPGQEVTFRFKATKAGTFVYHCAPGGIMIPWHVAMGMHGAIMVLPREGLSDGDGNPLHYDRAYYIGEADFYVPKD
AQGNYKRYNQLMESMEEVLTTMKTLLPSHIVFNGGVGALTGKHAMPAKVGESVLFIHSQANRDTRPHIIGGHGDYVWAAG
SFADRPQRDLETWMVAGGSAAAALYTFRQPGLHAYVNHNLIEAIMKGAAAHIKVDGEWDDNLMVQVRKPSAIA

Sequences:

>Translated_393_residues
MKNEQQSIEHGVDTNRRQFLGGSVGAGLVGLGLASGLMSSQANATTAGNKAKSDRMANYTADQLERVKQELVPPPMVPKH
DQVATGKPKVIEVRLEVEEKQIELENGVFAWVCAFNGSVPGPLIVCHQHDYVELTLVNPKTNVLSHNIDLHSATGALGGG
ELTLVAPGQEVTFRFKATKAGTFVYHCAPGGIMIPWHVAMGMHGAIMVLPREGLSDGDGNPLHYDRAYYIGEADFYVPKD
AQGNYKRYNQLMESMEEVLTTMKTLLPSHIVFNGGVGALTGKHAMPAKVGESVLFIHSQANRDTRPHIIGGHGDYVWAAG
SFADRPQRDLETWMVAGGSAAAALYTFRQPGLHAYVNHNLIEAIMKGAAAHIKVDGEWDDNLMVQVRKPSAIA
>Mature_393_residues
MKNEQQSIEHGVDTNRRQFLGGSVGAGLVGLGLASGLMSSQANATTAGNKAKSDRMANYTADQLERVKQELVPPPMVPKH
DQVATGKPKVIEVRLEVEEKQIELENGVFAWVCAFNGSVPGPLIVCHQHDYVELTLVNPKTNVLSHNIDLHSATGALGGG
ELTLVAPGQEVTFRFKATKAGTFVYHCAPGGIMIPWHVAMGMHGAIMVLPREGLSDGDGNPLHYDRAYYIGEADFYVPKD
AQGNYKRYNQLMESMEEVLTTMKTLLPSHIVFNGGVGALTGKHAMPAKVGESVLFIHSQANRDTRPHIIGGHGDYVWAAG
SFADRPQRDLETWMVAGGSAAAALYTFRQPGLHAYVNHNLIEAIMKGAAAHIKVDGEWDDNLMVQVRKPSAIA

Specific function: Unknown

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 plastocyanin-like domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011707
- InterPro:   IPR008972
- InterPro:   IPR001287
- InterPro:   IPR006311 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07732 Cu-oxidase_3 [H]

EC number: =1.7.2.1 [H]

Molecular weight: Translated: 42523; Mature: 42523

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEQQSIEHGVDTNRRQFLGGSVGAGLVGLGLASGLMSSQANATTAGNKAKSDRMANYT
CCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCC
ADQLERVKQELVPPPMVPKHDQVATGKPKVIEVRLEVEEKQIELENGVFAWVCAFNGSVP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECEEEEECCCEEEEEEEECCCCC
GPLIVCHQHDYVELTLVNPKTNVLSHNIDLHSATGALGGGELTLVAPGQEVTFRFKATKA
CCEEEEECCCEEEEEEECCCCCEEEECCEEECCCCCCCCCEEEEEECCCEEEEEEEEECC
GTFVYHCAPGGIMIPWHVAMGMHGAIMVLPREGLSDGDGNPLHYDRAYYIGEADFYVPKD
CEEEEEECCCCEEEEEHHHCCCCCEEEEEECCCCCCCCCCCEEECCEEEECCCCEECCCC
AQGNYKRYNQLMESMEEVLTTMKTLLPSHIVFNGGVGALTGKHAMPAKVGESVLFIHSQA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCEEEEEEECC
NRDTRPHIIGGHGDYVWAAGSFADRPQRDLETWMVAGGSAAAALYTFRQPGLHAYVNHNL
CCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHEEECCCHHEEEEEECCCCCEEEHHHHH
IEAIMKGAAAHIKVDGEWDDNLMVQVRKPSAIA
HHHHHCCCEEEEEECCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKNEQQSIEHGVDTNRRQFLGGSVGAGLVGLGLASGLMSSQANATTAGNKAKSDRMANYT
CCCHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCC
ADQLERVKQELVPPPMVPKHDQVATGKPKVIEVRLEVEEKQIELENGVFAWVCAFNGSVP
HHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEEECEEEEECCCEEEEEEEECCCCC
GPLIVCHQHDYVELTLVNPKTNVLSHNIDLHSATGALGGGELTLVAPGQEVTFRFKATKA
CCEEEEECCCEEEEEEECCCCCEEEECCEEECCCCCCCCCEEEEEECCCEEEEEEEEECC
GTFVYHCAPGGIMIPWHVAMGMHGAIMVLPREGLSDGDGNPLHYDRAYYIGEADFYVPKD
CEEEEEECCCCEEEEEHHHCCCCCEEEEEECCCCCCCCCCCEEECCEEEECCCCEECCCC
AQGNYKRYNQLMESMEEVLTTMKTLLPSHIVFNGGVGALTGKHAMPAKVGESVLFIHSQA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHCCEEEEEEECC
NRDTRPHIIGGHGDYVWAAGSFADRPQRDLETWMVAGGSAAAALYTFRQPGLHAYVNHNL
CCCCCCCEECCCCCEEEECCCCCCCCHHHHHHHEEECCCHHEEEEEECCCCCEEEHHHHH
IEAIMKGAAAHIKVDGEWDDNLMVQVRKPSAIA
HHHHHCCCEEEEEECCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]