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The map label for this gene is puuB [H]

Identifier: 119775770

GI number: 119775770

Start: 3160726

End: 3162012

Strand: Reverse

Name: puuB [H]

Synonym: Sama_2638

Alternate gene names: 119775770

Gene position: 3162012-3160726 (Counterclockwise)

Preceding gene: 119775771

Following gene: 119775769

Centisome position: 73.43

GC content: 54.47

Gene sequence:

>1287_bases
ATGTCTGCTACACCTCATACCGAGTCCTACTATGCCGCTTCCGCCAATGACACCCGCATTCGTCCTCGTCTTGAGGAGCA
TCTTGAAACCGACGTGTGTGTTATAGGCGCAGGCTATACGGGTCTGTCCTCGGCATTGCATTTGCTTGAAAGTGGTTTCA
AGGTCGTGGTTTTGGAGGCTGCCCGAATTGGTTGGGGCGCCTCGGGTCGCAATGGCGGCCAAATCGTTAACAGCTATAGC
CGAGACATTGATGTCATTGAAAAAACCGTTGGCAAGGAAAAGGGCAAGCTGTTCGGTGAAATGGCATTTGAAGGCGGTCG
CATCATTCGCGATCGCATCGCAAAATATAATATCGACTGCGACCTGAAAAACGGCGGTGTGTTTGCCGCACTCAACCAGA
AGCAGATGGGCCACCTCAAAGCGCAAAAAGCGCTGTGGGAAGCTCACGGTCATACCAATCAGCTGGAAATCCTCGATGAA
GCCGGTATCCGTAATGTGGTGAATACTGAACGTTACGTCGGCGGTATGCTGGATAAGAGCGGTGGTCACATTCATCCGTT
GAATTTGGCGCTGGGCGAAGCCCGTGCGGTGGAAAGCCTCGGCGGTAAAATCTTTGAAGACTCAGCCGTGCTCGATGTAG
TGGAAGGCGAGCCTGCTATTGTCAAAACCGAGTTCGGTCAGGTGAAGGCCCGTTTTGTGGTGGTGGCCGGTAATGCTTAC
CTTGGCAAGCTGATGCCTGAGCTGATGGCCAAGTCCATGCCCTGCGGCACCCAGGTGATTACCACCGAGCCCCTGAGCGA
TGAGATGGCAAATAGCCTGCTGCCACAGGACTACTGTGTTGAAGACTGTAACTACCTGCTGGACTATTTCCGCCTCTCCG
GCGACAAGCGTCTGATTTACGGTGGTGGCGTGGTGTATGGCGCCCGCGACCCGGACAACATCAAGTCCATTATTCTGCCG
AAAATGCTGGAGACTTTCCCGCAGCTTAAAGGTGTGCGTATCGACTACACCTGGACGGGTAACTTCCTGCTGACCCTGTC
GCGTCTGCCTCAGGTCGGCCGTATTGGCAAGAACATCTATTACTCCCAGGGCTGCAGTGGCCACGGTGTAACCTACACCC
ATCTGGCGGGCAAACTCATTGCCGAGGTGCTTAATGGTCAGGCTACCCGCTTCGATGTCTTTGCTGAACTGCCCCATTAT
CCGTTCCCGGGTGGCCACGCGCTGCGGGTGCCATTCAGTGCCATCGGTGCCTGGTATTACACCATGCGTGACAAGCTTGG
CATTTAA

Upstream 100 bases:

>100_bases
AATTCAAAATTATCCTTCACTAGCGCTAGAGTTTCCCTTGGTGAACGTGTAGGATGAACTGGTTGTTTAAAATAATGAAC
ATAAGAGAGAAGGAAGCATC

Downstream 100 bases:

>100_bases
CAGAATTTTCGGCGTAAGTGCCCAGTAACAGGTATCAAGTAACAAGGAGTCAGCTGTGCAGCTCAAACGTCCTTCACTAT
TGAAAACAAAATGTTATATC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSATPHTESYYAASANDTRIRPRLEEHLETDVCVIGAGYTGLSSALHLLESGFKVVVLEAARIGWGASGRNGGQIVNSYS
RDIDVIEKTVGKEKGKLFGEMAFEGGRIIRDRIAKYNIDCDLKNGGVFAALNQKQMGHLKAQKALWEAHGHTNQLEILDE
AGIRNVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLDVVEGEPAIVKTEFGQVKARFVVVAGNAY
LGKLMPELMAKSMPCGTQVITTEPLSDEMANSLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPDNIKSIILP
KMLETFPQLKGVRIDYTWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEVLNGQATRFDVFAELPHY
PFPGGHALRVPFSAIGAWYYTMRDKLGI

Sequences:

>Translated_428_residues
MSATPHTESYYAASANDTRIRPRLEEHLETDVCVIGAGYTGLSSALHLLESGFKVVVLEAARIGWGASGRNGGQIVNSYS
RDIDVIEKTVGKEKGKLFGEMAFEGGRIIRDRIAKYNIDCDLKNGGVFAALNQKQMGHLKAQKALWEAHGHTNQLEILDE
AGIRNVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLDVVEGEPAIVKTEFGQVKARFVVVAGNAY
LGKLMPELMAKSMPCGTQVITTEPLSDEMANSLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPDNIKSIILP
KMLETFPQLKGVRIDYTWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEVLNGQATRFDVFAELPHY
PFPGGHALRVPFSAIGAWYYTMRDKLGI
>Mature_427_residues
SATPHTESYYAASANDTRIRPRLEEHLETDVCVIGAGYTGLSSALHLLESGFKVVVLEAARIGWGASGRNGGQIVNSYSR
DIDVIEKTVGKEKGKLFGEMAFEGGRIIRDRIAKYNIDCDLKNGGVFAALNQKQMGHLKAQKALWEAHGHTNQLEILDEA
GIRNVVNTERYVGGMLDKSGGHIHPLNLALGEARAVESLGGKIFEDSAVLDVVEGEPAIVKTEFGQVKARFVVVAGNAYL
GKLMPELMAKSMPCGTQVITTEPLSDEMANSLLPQDYCVEDCNYLLDYFRLSGDKRLIYGGGVVYGARDPDNIKSIILPK
MLETFPQLKGVRIDYTWTGNFLLTLSRLPQVGRIGKNIYYSQGCSGHGVTYTHLAGKLIAEVLNGQATRFDVFAELPHYP
FPGGHALRVPFSAIGAWYYTMRDKLGI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=65.0234741784037, Blast_Score=589, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46834; Mature: 46703

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATPHTESYYAASANDTRIRPRLEEHLETDVCVIGAGYTGLSSALHLLESGFKVVVLEA
CCCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSYSRDIDVIEKTVGKEKGKLFGEMAFEGGRIIRDRIAKYNIDC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
DLKNGGVFAALNQKQMGHLKAQKALWEAHGHTNQLEILDEAGIRNVVNTERYVGGMLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCHHHHHHHHHHCCCCCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLDVVEGEPAIVKTEFGQVKARFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCCEEEEECCCCCEEEECCCCEEEEEEEEECCHH
LGKLMPELMAKSMPCGTQVITTEPLSDEMANSLLPQDYCVEDCNYLLDYFRLSGDKRLIY
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPDNIKSIILPKMLETFPQLKGVRIDYTWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLIAEVLNGQATRFDVFAELPHYPFPGGHALRVPFSAIGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEHEECCCCCCCCCCCEEECCHHHHHHHHH
TMRDKLGI
HHHHHCCC
>Mature Secondary Structure 
SATPHTESYYAASANDTRIRPRLEEHLETDVCVIGAGYTGLSSALHLLESGFKVVVLEA
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
ARIGWGASGRNGGQIVNSYSRDIDVIEKTVGKEKGKLFGEMAFEGGRIIRDRIAKYNIDC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHCCCEE
DLKNGGVFAALNQKQMGHLKAQKALWEAHGHTNQLEILDEAGIRNVVNTERYVGGMLDKS
EECCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEHHCCCHHHHHHHHHHCCCCCCC
GGHIHPLNLALGEARAVESLGGKIFEDSAVLDVVEGEPAIVKTEFGQVKARFVVVAGNAY
CCEEEEEHEEHHHHHHHHHHCCEEECCCCEEEEECCCCCEEEECCCCEEEEEEEEECCHH
LGKLMPELMAKSMPCGTQVITTEPLSDEMANSLLPQDYCVEDCNYLLDYFRLSGDKRLIY
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GGGVVYGARDPDNIKSIILPKMLETFPQLKGVRIDYTWTGNFLLTLSRLPQVGRIGKNIY
ECEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEHHCCCHHHHCCCEE
YSQGCSGHGVTYTHLAGKLIAEVLNGQATRFDVFAELPHYPFPGGHALRVPFSAIGAWYY
ECCCCCCCCEEHHHHHHHHHHHHHCCCCEEEEHEECCCCCCCCCCCEEECCHHHHHHHHH
TMRDKLGI
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]