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The map label for this gene is pleD [H]

Identifier: 119775755

GI number: 119775755

Start: 3141040

End: 3142920

Strand: Direct

Name: pleD [H]

Synonym: Sama_2623

Alternate gene names: 119775755

Gene position: 3141040-3142920 (Clockwise)

Preceding gene: 119775753

Following gene: 119775757

Centisome position: 72.94

GC content: 50.72

Gene sequence:

>1881_bases
ATGAAATTTGATTTTCCAAAGTCCATTCTTTGGGCCATTTTTTTACTTATCCTCTGGCTGCCCGCGCATGCCGGTGATTT
GACGCTGGACGAGATAGACAGGCTGTTCAGCCAACTGGAAAGCGATCCATCTCAGTTTGATTCCATTCCCGAGCAGGTTG
AACTGCTGAAACAAAATATTGTTGCCGACGATATTCCTCGCACACTGCGCCTTAAACGAACGCTCTGTTGGTCAACTGAC
CTGACTCAGGAGAATGCCCTGGAGCATGGGCTGACTTATGCCAACGAACAACTGGCACTGCCGATGGTTCAGGCGGATAT
CCCAACTCAAGCCGATTTGATGCTCTGCCGGGGCTGGTTATTACAGCAGAAAGGCGTTATGGCCGACGCCATGAGTGATT
ACAATCGCTCGGTTTCTCTTGCCTATCAATCGGAAGAGCCCCGTCTGATTGCAGATAGCCGCAGTCTCAGGGGAGCCCTG
CTTTCCTATCAGGGCAACTTTGCCGATGCGCTGGAAGACCTGATTACGTCCCAACATCTTTATGAGAGCCTCAATTTAAC
CTACTGGGCCAGATACAATCTCCTTGAGCTTGCCACCAGTTATCGCCGTTTCGGCGACCCGGACACGGCTATTCGGTATT
ACGAAGACCTCTTGCAAAAGTACACTGAAGCCAAAGACACGTTTGCCATGGTAGGCGTCAAGGCCGAAATGGCCATGGCG
TATGAAACGCTGGAGGATTTCGAGAAAGCGCTCGTCTACTACAAAGATGTATTGGCTGGCTGGCTGGCGCAAAAAGACGA
GATCAGTGTGGCCGCCACCCGGGTAAATATGAGTGGCACCCTCATAAAACTCGGCAGACTTAAAGAGGCAAAGGCTGAGT
TGGAACTGGCCTTTCCCGAATTAAAACCCGAGCACGTAGGGGTTTACGCATTCGAGCATCTGTTTATGGCGCAAATACTG
CTCGCAGAGGGTAAACCGCTGGATGCGCTGCCGCTGCTGGAAGTTGGCGAAGAAAGCTTTCGCAATGCGCAAAACAGCCG
TGGATTAGCCGAGCTCTATGCCACCAAAAGCCAGGTCTACGCGGCATTGGAAGATTGGGCCAACGCCTTTTATACCCTTG
AGATACAAAACGCACTCCATACTCAACTGGACAACCAGTTGCAGTCACAACGCACCACAGAGATGCGAACCCGATTCGAT
ACCGACAGAATTGAAAATGAAAACCGCCAGCTGGTTGCTTATCAACGCTTGCGGGAAAAAGAAATGGCCATACTGCAGGA
AAATAAATTTCTGCAATTCGTCATTATCGTTATGGGCATCATCATCCTCAGCATTGTGTCTATTTTTGCTTACAAGCAGG
CACAGCGCTCTAAACTGATGGAACGGCTCGCCCTCACCGATCACCTGACACAACTTGCCAATCGTCGGCACACCTACCAT
GAAGGCGAGATCATGTTCCGAAGCGCAAGACCTGGCAGTCCGTTGTCTGTCATCTTGTTTGATGCCGACCATTTCAAAAA
AATCAATGACCACTTCGGTCATGATGTTGGTGATAAAGTCTTGATTGCCATGGCTCAAAGTAGCGCTGGGCAAATGCGTA
GAGGCGATTTGGTTGGCCGCATTGGCGGTGAAGAGTTTCTGGCGTTACTGCCACACACCACTTTGGAACAGGCGGTTGAA
ATTGCTGAGCGCCTGCGGTTAACCATAGAAACGACGGATTTGTCAGAGGTCGCCCCTGGCCTTGGCATGAGCATCAGCGC
GGGGGTCGCCACCCTGGATAGCAGCAGAGATAAAAACTTTTCCAGCTTCCTGAACCGGGCAGATCACGCGCTTTATCGTG
CCAAACACAATGGTCGTAACCGGGTGGAAAAAGACGATTAA

Upstream 100 bases:

>100_bases
GCTGGTTTTCACGCTGCACAATAAGAAAAACTGGGAAACTCCACGACATACGGTTATGATTTGCACATAACAAAAAGTAA
CAAATCGGCAACAGATTTAG

Downstream 100 bases:

>100_bases
TCTTCTGGCTTCAAATGCCAGAGAATGTGGCTGGACGTATTTTCCGCGGACATGTTAAACCACTTCTCGTACAGCCTCAC
AAAATCCCCTTCTTCCTGAA

Product: cyclic nucleotide phosphodiesterase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKFDFPKSILWAIFLLILWLPAHAGDLTLDEIDRLFSQLESDPSQFDSIPEQVELLKQNIVADDIPRTLRLKRTLCWSTD
LTQENALEHGLTYANEQLALPMVQADIPTQADLMLCRGWLLQQKGVMADAMSDYNRSVSLAYQSEEPRLIADSRSLRGAL
LSYQGNFADALEDLITSQHLYESLNLTYWARYNLLELATSYRRFGDPDTAIRYYEDLLQKYTEAKDTFAMVGVKAEMAMA
YETLEDFEKALVYYKDVLAGWLAQKDEISVAATRVNMSGTLIKLGRLKEAKAELELAFPELKPEHVGVYAFEHLFMAQIL
LAEGKPLDALPLLEVGEESFRNAQNSRGLAELYATKSQVYAALEDWANAFYTLEIQNALHTQLDNQLQSQRTTEMRTRFD
TDRIENENRQLVAYQRLREKEMAILQENKFLQFVIIVMGIIILSIVSIFAYKQAQRSKLMERLALTDHLTQLANRRHTYH
EGEIMFRSARPGSPLSVILFDADHFKKINDHFGHDVGDKVLIAMAQSSAGQMRRGDLVGRIGGEEFLALLPHTTLEQAVE
IAERLRLTIETTDLSEVAPGLGMSISAGVATLDSSRDKNFSSFLNRADHALYRAKHNGRNRVEKDD

Sequences:

>Translated_626_residues
MKFDFPKSILWAIFLLILWLPAHAGDLTLDEIDRLFSQLESDPSQFDSIPEQVELLKQNIVADDIPRTLRLKRTLCWSTD
LTQENALEHGLTYANEQLALPMVQADIPTQADLMLCRGWLLQQKGVMADAMSDYNRSVSLAYQSEEPRLIADSRSLRGAL
LSYQGNFADALEDLITSQHLYESLNLTYWARYNLLELATSYRRFGDPDTAIRYYEDLLQKYTEAKDTFAMVGVKAEMAMA
YETLEDFEKALVYYKDVLAGWLAQKDEISVAATRVNMSGTLIKLGRLKEAKAELELAFPELKPEHVGVYAFEHLFMAQIL
LAEGKPLDALPLLEVGEESFRNAQNSRGLAELYATKSQVYAALEDWANAFYTLEIQNALHTQLDNQLQSQRTTEMRTRFD
TDRIENENRQLVAYQRLREKEMAILQENKFLQFVIIVMGIIILSIVSIFAYKQAQRSKLMERLALTDHLTQLANRRHTYH
EGEIMFRSARPGSPLSVILFDADHFKKINDHFGHDVGDKVLIAMAQSSAGQMRRGDLVGRIGGEEFLALLPHTTLEQAVE
IAERLRLTIETTDLSEVAPGLGMSISAGVATLDSSRDKNFSSFLNRADHALYRAKHNGRNRVEKDD
>Mature_626_residues
MKFDFPKSILWAIFLLILWLPAHAGDLTLDEIDRLFSQLESDPSQFDSIPEQVELLKQNIVADDIPRTLRLKRTLCWSTD
LTQENALEHGLTYANEQLALPMVQADIPTQADLMLCRGWLLQQKGVMADAMSDYNRSVSLAYQSEEPRLIADSRSLRGAL
LSYQGNFADALEDLITSQHLYESLNLTYWARYNLLELATSYRRFGDPDTAIRYYEDLLQKYTEAKDTFAMVGVKAEMAMA
YETLEDFEKALVYYKDVLAGWLAQKDEISVAATRVNMSGTLIKLGRLKEAKAELELAFPELKPEHVGVYAFEHLFMAQIL
LAEGKPLDALPLLEVGEESFRNAQNSRGLAELYATKSQVYAALEDWANAFYTLEIQNALHTQLDNQLQSQRTTEMRTRFD
TDRIENENRQLVAYQRLREKEMAILQENKFLQFVIIVMGIIILSIVSIFAYKQAQRSKLMERLALTDHLTQLANRRHTYH
EGEIMFRSARPGSPLSVILFDADHFKKINDHFGHDVGDKVLIAMAQSSAGQMRRGDLVGRIGGEEFLALLPHTTLEQAVE
IAERLRLTIETTDLSEVAPGLGMSISAGVATLDSSRDKNFSSFLNRADHALYRAKHNGRNRVEKDD

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=40.6976744186046, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI87081881, Length=183, Percent_Identity=38.2513661202186, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787262, Length=187, Percent_Identity=36.3636363636364, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=161, Percent_Identity=40.3726708074534, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786584, Length=200, Percent_Identity=34, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787802, Length=191, Percent_Identity=32.9842931937173, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1788085, Length=185, Percent_Identity=35.1351351351351, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=31.4102564102564, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=29.8913043478261, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=155, Percent_Identity=31.6129032258064, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=163, Percent_Identity=28.2208588957055, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787541, Length=185, Percent_Identity=31.8918918918919, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 71163; Mature: 71163

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFDFPKSILWAIFLLILWLPAHAGDLTLDEIDRLFSQLESDPSQFDSIPEQVELLKQNI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
VADDIPRTLRLKRTLCWSTDLTQENALEHGLTYANEQLALPMVQADIPTQADLMLCRGWL
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCCCCCEEHCCCCCCHHHHHHHHHHH
LQQKGVMADAMSDYNRSVSLAYQSEEPRLIADSRSLRGALLSYQGNFADALEDLITSQHL
HHHCCCHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YESLNLTYWARYNLLELATSYRRFGDPDTAIRYYEDLLQKYTEAKDTFAMVGVKAEMAMA
HHHCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLEDFEKALVYYKDVLAGWLAQKDEISVAATRVNMSGTLIKLGRLKEAKAELELAFPE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHEECCC
LKPEHVGVYAFEHLFMAQILLAEGKPLDALPLLEVGEESFRNAQNSRGLAELYATKSQVY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHCCCCCCCHHHHHHHHHHHH
AALEDWANAFYTLEIQNALHTQLDNQLQSQRTTEMRTRFDTDRIENENRQLVAYQRLREK
HHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHH
EMAILQENKFLQFVIIVMGIIILSIVSIFAYKQAQRSKLMERLALTDHLTQLANRRHTYH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGEIMFRSARPGSPLSVILFDADHFKKINDHFGHDVGDKVLIAMAQSSAGQMRRGDLVGR
CCCEEEECCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHEEEEHHCCCCCCCCCCHHCC
IGGEEFLALLPHTTLEQAVEIAERLRLTIETTDLSEVAPGLGMSISAGVATLDSSRDKNF
CCHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCHHHHCCCCCCCHHCCHHHHCCCCCCCH
SSFLNRADHALYRAKHNGRNRVEKDD
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFDFPKSILWAIFLLILWLPAHAGDLTLDEIDRLFSQLESDPSQFDSIPEQVELLKQNI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
VADDIPRTLRLKRTLCWSTDLTQENALEHGLTYANEQLALPMVQADIPTQADLMLCRGWL
HHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCHHCCCCCCCEEHCCCCCCHHHHHHHHHHH
LQQKGVMADAMSDYNRSVSLAYQSEEPRLIADSRSLRGALLSYQGNFADALEDLITSQHL
HHHCCCHHHHHHHCCCEEEEEEECCCCCEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHH
YESLNLTYWARYNLLELATSYRRFGDPDTAIRYYEDLLQKYTEAKDTFAMVGVKAEMAMA
HHHCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLEDFEKALVYYKDVLAGWLAQKDEISVAATRVNMSGTLIKLGRLKEAKAELELAFPE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHEECCC
LKPEHVGVYAFEHLFMAQILLAEGKPLDALPLLEVGEESFRNAQNSRGLAELYATKSQVY
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHHCCCCCCCHHHHHHHHHHHH
AALEDWANAFYTLEIQNALHTQLDNQLQSQRTTEMRTRFDTDRIENENRQLVAYQRLREK
HHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHHH
EMAILQENKFLQFVIIVMGIIILSIVSIFAYKQAQRSKLMERLALTDHLTQLANRRHTYH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGEIMFRSARPGSPLSVILFDADHFKKINDHFGHDVGDKVLIAMAQSSAGQMRRGDLVGR
CCCEEEECCCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHEEEEHHCCCCCCCCCCHHCC
IGGEEFLALLPHTTLEQAVEIAERLRLTIETTDLSEVAPGLGMSISAGVATLDSSRDKNF
CCHHHHHHHCCCHHHHHHHHHHHHHEEEEEECCHHHHCCCCCCCHHCCHHHHCCCCCCCH
SSFLNRADHALYRAKHNGRNRVEKDD
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA