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The map label for this gene is clpB [H]

Identifier: 119775735

GI number: 119775735

Start: 3114894

End: 3117467

Strand: Reverse

Name: clpB [H]

Synonym: Sama_2603

Alternate gene names: 119775735

Gene position: 3117467-3114894 (Counterclockwise)

Preceding gene: 119775736

Following gene: 119775734

Centisome position: 72.4

GC content: 55.4

Gene sequence:

>2574_bases
ATGCGACTCGATCGTATGACCAACAAATTCCAGATTGCCATATCTGATGCTCAGTCTCTGGCCCTTGGCAGAGACCACCA
ATTTATCGAACCCGTTCACCTGATGATGGCGCTGCTGAATCAGGACGGTGGCTCCATTCATCCTTTATTAACCCAGTCCG
GCATTCGGGTGAGTAACCTGCGCTCATTATTGTCTCAGGAGTTGGAACGCCAACCACAGGTGGAAGGCACCGGCGGTGAT
GTGCAGCTGTCGCAGGCGCTTATCCGTTTGCTGAACCTGTGCGACAAGCTGTCGCAAAAGCGTAAAGACAAATACATCTC
CAGCGAGCTGTTTGTGCTTGCAGCGCTGGAAGGTAACGACACCTTGGCGCAAATCCTCAAGCAGGCGGGCGCGACCAAAG
AGCGACTGGAGCAAACCATTGAAGAAATCCGCGCTGGCCAGAAGGTGGACGACCCCAACGCCGAAGATAATCGTCAGGCG
CTGAAAAAGTACACAGTCGACCTGACCGAGCGCGCCGAGCAGGGCAAGCTTGACCCTGTGATTGGCCGTGACGATGAAAT
TCGCCGCACCATTCAGGTATTGCAGCGCCGCAGTAAAAACAATCCTGTCTTGATAGGTGAGCCCGGTGTGGGTAAAACCG
CCATCGTCGAGGGGCTGGCCCAGCGGATTGTTAATGGCGAGGTGCCGGAAGGCATCAAAAACAAGCGGGTGTTGTCCCTG
GATATGGGCGCGCTGGTGGCCGGTGCCAAATACCGCGGTGAGTTTGAAGAGCGCTTGAAAGCGGTGCTCAATGAGCTGGC
TCAGGAAGAAGGCCAGGTTATCCTCTTTATTGATGAGCTGCACACCATGGTGGGCGCCGGTAAGAGCGATGGCGCCATGG
ACGCAGGTAATATGCTCAAGCCTGCGCTGGCCCGCGGTGAGCTGCACTGCGTCGGCGCCACTACTCTGGATGAGTACCGC
CAGTATGTGGAAAAAGATGCCGCCCTTGAGCGCCGTTTCCAAAAAGTGCTGGTGGACGAGCCCACAGTGGAAGACACCAT
CGCTATTTTGCGTGGTCTCAAGGAGCGTTACGAGCTGCACCACCATGTGGAAATTACCGATCCTGCCATTGTGGCGGCAG
CGAGCATGTCACACCGCTATGTGTCAGACCGCAAATTGCCTGACAAGGCGATCGATTTGATTGATGAAGCGGCTTCCAGT
ATCCGGATGCAGATTGACTCCAAGCCTGAGTCGCTTGATCGCCTCGAACGCCGCGCTATTCAGCTCAAGCTCGAAGAGCA
GGCACTGGCAAAGGAAAACGACGAGGCCAGCCGCAAGCGTTTGGAGCATTTGCGCCATGAGCTGAAAGAGGTAGAAGCCA
AGGCCAATGAGCTCAATGAAATCTGGCGTACCGAGAAAGCCGCCCTGGCGGGCACCCAGCATATTAAAGCAGACCTCGAG
CAGGCGCGTATGGATCTGGAAGTTGCCCGCCGGGCGAGCGATTTGACCCGCATGTCTGAGCTGCAATATGGCCGTATCCC
TGAGCTTGAAAAACAGCTCGACTTGGCGGCACAAGCTGAAATGCAGGACATGAAGCTGCTGCGCAACAAGGTGACCGATG
TGGAAATTGCCGAGGTGCTGTCCAAGGCAACCGGTATTCCCGTGGCCAAAATGCTTGAAGGCGAGCGTGAAAAGCTGCTG
CAAATGGAACATGCGCTGCATGAGCGGGTGATTGGTCAGAATGAGGCGGTGGATGCCGTGGCGAATGCAATTCGCAGAAG
TCGCGCCGGGCTTGCTGACCCTAACCGCCCAATCGGCTCCTTTTTGTTCCTTGGCCCGACCGGGGTGGGTAAAACCGAGC
TGTGTAAATCACTGGCGAAGTTTTTGTTCGACACAGAGTCGGCGCTGGTGCGTATCGATATGTCCGAGTTTATGGAGAAG
CATTCGGTTTCCCGGCTGGTGGGGGCGCCTCCGGGATACGTTGGCTACGAAGAAGGCGGTTATCTTACCGAGGCGGTGCG
CCGTAAGCCGTATTCGGTGATTCTGCTCGATGAGGTGGAAAAGGCGCATCCGGATGTGTTCAACATCCTGTTGCAGGTGC
TTGACGATGGTCGTCTGACCGATGGCCAGGGCCGCACTGTGGATTTCCGCAATACCGTGGTGATCATGACCTCGAACCTC
GGCTCAGACATTATTCAGGAGCGCTTTGGTACTCTGGATTATGCCCAGATGAAGCAAGAGGTCATGAATGTGGTGGTACA
CAGCTTCCGGCCAGAGTTTTTAAACCGGATTGATGAAACGGTTGTGTTCCACCCGCTGGACGCAGAGCACATCAAGAACA
TAGCCTCCATCCAAATCGAGTCGCTGCGTAAGCGTTTGGCAGAGCGAGAGTTTGAACTCGAACTCAGTGATGAAGCGCTG
GCCTTTATCGCCAGAGCCGGTTTTGATCCTGTGTATGGAGCGCGGCCTCTGAAGCGGGCGTTACAACAAGAGGTTGAAAA
TCCGCTGGCGCAGAAACTGCTGCGGGGTGAATTTATCCCCGGTAAACCCATTCGGGTAGACTGTCAGGATGCTGAGTTGG
TGTTCGCCCAATAA

Upstream 100 bases:

>100_bases
GGCAGCCCCATCTTTTAGATAACTACTCTCAGGTGACCATCCTTAGGTAACCATTCTTGGGTAACTAAAATTCAATGCGG
TCCTAGTTACAGGAGGCTGT

Downstream 100 bases:

>100_bases
CCAGACAAGCACACTCACGGGAGACTGAGTCAGAAAGTCTTGATGTATTGGTGAGCCGCAATTCGGACGATAAAGAAGAG
GGAGCCAATTGGCTCCCTCT

Product: clpB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPVHLMMALLNQDGGSIHPLLTQSGIRVSNLRSLLSQELERQPQVEGTGGD
VQLSQALIRLLNLCDKLSQKRKDKYISSELFVLAALEGNDTLAQILKQAGATKERLEQTIEEIRAGQKVDDPNAEDNRQA
LKKYTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELAQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYVEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYVSDRKLPDKAIDLIDEAASS
IRMQIDSKPESLDRLERRAIQLKLEEQALAKENDEASRKRLEHLRHELKEVEAKANELNEIWRTEKAALAGTQHIKADLE
QARMDLEVARRASDLTRMSELQYGRIPELEKQLDLAAQAEMQDMKLLRNKVTDVEIAEVLSKATGIPVAKMLEGEREKLL
QMEHALHERVIGQNEAVDAVANAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDIIQERFGTLDYAQMKQEVMNVVVHSFRPEFLNRIDETVVFHPLDAEHIKNIASIQIESLRKRLAEREFELELSDEAL
AFIARAGFDPVYGARPLKRALQQEVENPLAQKLLRGEFIPGKPIRVDCQDAELVFAQ

Sequences:

>Translated_857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPVHLMMALLNQDGGSIHPLLTQSGIRVSNLRSLLSQELERQPQVEGTGGD
VQLSQALIRLLNLCDKLSQKRKDKYISSELFVLAALEGNDTLAQILKQAGATKERLEQTIEEIRAGQKVDDPNAEDNRQA
LKKYTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELAQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYVEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYVSDRKLPDKAIDLIDEAASS
IRMQIDSKPESLDRLERRAIQLKLEEQALAKENDEASRKRLEHLRHELKEVEAKANELNEIWRTEKAALAGTQHIKADLE
QARMDLEVARRASDLTRMSELQYGRIPELEKQLDLAAQAEMQDMKLLRNKVTDVEIAEVLSKATGIPVAKMLEGEREKLL
QMEHALHERVIGQNEAVDAVANAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDIIQERFGTLDYAQMKQEVMNVVVHSFRPEFLNRIDETVVFHPLDAEHIKNIASIQIESLRKRLAEREFELELSDEAL
AFIARAGFDPVYGARPLKRALQQEVENPLAQKLLRGEFIPGKPIRVDCQDAELVFAQ
>Mature_857_residues
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPVHLMMALLNQDGGSIHPLLTQSGIRVSNLRSLLSQELERQPQVEGTGGD
VQLSQALIRLLNLCDKLSQKRKDKYISSELFVLAALEGNDTLAQILKQAGATKERLEQTIEEIRAGQKVDDPNAEDNRQA
LKKYTVDLTERAEQGKLDPVIGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGIKNKRVLSL
DMGALVAGAKYRGEFEERLKAVLNELAQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLKPALARGELHCVGATTLDEYR
QYVEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHHVEITDPAIVAAASMSHRYVSDRKLPDKAIDLIDEAASS
IRMQIDSKPESLDRLERRAIQLKLEEQALAKENDEASRKRLEHLRHELKEVEAKANELNEIWRTEKAALAGTQHIKADLE
QARMDLEVARRASDLTRMSELQYGRIPELEKQLDLAAQAEMQDMKLLRNKVTDVEIAEVLSKATGIPVAKMLEGEREKLL
QMEHALHERVIGQNEAVDAVANAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKSLAKFLFDTESALVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDIIQERFGTLDYAQMKQEVMNVVVHSFRPEFLNRIDETVVFHPLDAEHIKNIASIQIESLRKRLAEREFELELSDEAL
AFIARAGFDPVYGARPLKRALQQEVENPLAQKLLRGEFIPGKPIRVDCQDAELVFAQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=324, Percent_Identity=37.962962962963, Blast_Score=214, Evalue=3e-55,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=77.4795799299883, Blast_Score=1378, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=343, Percent_Identity=41.399416909621, Blast_Score=271, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6320464, Length=710, Percent_Identity=52.8169014084507, Blast_Score=771, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=838, Percent_Identity=42.8400954653938, Blast_Score=667, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96073; Mature: 96073

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPVHLMMALLNQDGGSIHPLLTQSGIRVSNL
CCCCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHH
RSLLSQELERQPQVEGTGGDVQLSQALIRLLNLCDKLSQKRKDKYISSELFVLAALEGND
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
TLAQILKQAGATKERLEQTIEEIRAGQKVDDPNAEDNRQALKKYTVDLTERAEQGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGIKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLNELAQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLK
EHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYVEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASMSHRYVSDRKLPDKAIDLIDEAASSIRMQIDSKPESLDRLERRAI
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKLEEQALAKENDEASRKRLEHLRHELKEVEAKANELNEIWRTEKAALAGTQHIKADLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARMDLEVARRASDLTRMSELQYGRIPELEKQLDLAAQAEMQDMKLLRNKVTDVEIAEVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SKATGIPVAKMLEGEREKLLQMEHALHERVIGQNEAVDAVANAIRRSRAGLADPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLAKFLFDTESALVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDIIQERFGTLDYAQMKQEVMNVVVHSFRPEFLNRIDETVVFHPLDAEHIKNIASIQIE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
SLRKRLAEREFELELSDEALAFIARAGFDPVYGARPLKRALQQEVENPLAQKLLRGEFIP
HHHHHHHHHHCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCC
GKPIRVDCQDAELVFAQ
CCCEEEECCCCCEEECC
>Mature Secondary Structure
MRLDRMTNKFQIAISDAQSLALGRDHQFIEPVHLMMALLNQDGGSIHPLLTQSGIRVSNL
CCCCCCCCEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHH
RSLLSQELERQPQVEGTGGDVQLSQALIRLLNLCDKLSQKRKDKYISSELFVLAALEGND
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCH
TLAQILKQAGATKERLEQTIEEIRAGQKVDDPNAEDNRQALKKYTVDLTERAEQGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
IGRDDEIRRTIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGIKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLNELAQEEGQVILFIDELHTMVGAGKSDGAMDAGNMLK
EHHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHH
PALARGELHCVGATTLDEYRQYVEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELH
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVEITDPAIVAAASMSHRYVSDRKLPDKAIDLIDEAASSIRMQIDSKPESLDRLERRAI
HCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHH
QLKLEEQALAKENDEASRKRLEHLRHELKEVEAKANELNEIWRTEKAALAGTQHIKADLE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QARMDLEVARRASDLTRMSELQYGRIPELEKQLDLAAQAEMQDMKLLRNKVTDVEIAEVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
SKATGIPVAKMLEGEREKLLQMEHALHERVIGQNEAVDAVANAIRRSRAGLADPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKSLAKFLFDTESALVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRKPYSVILLDEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDIIQERFGTLDYAQMKQEVMNVVVHSFRPEFLNRIDETVVFHPLDAEHIKNIASIQIE
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHH
SLRKRLAEREFELELSDEALAFIARAGFDPVYGARPLKRALQQEVENPLAQKLLRGEFIP
HHHHHHHHHHCCEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCC
GKPIRVDCQDAELVFAQ
CCCEEEECCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]