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The map label for this gene is mltB [H]

Identifier: 119775722

GI number: 119775722

Start: 3098349

End: 3099338

Strand: Direct

Name: mltB [H]

Synonym: Sama_2590

Alternate gene names: 119775722

Gene position: 3098349-3099338 (Clockwise)

Preceding gene: 119775721

Following gene: 119775723

Centisome position: 71.95

GC content: 53.54

Gene sequence:

>990_bases
ATGAGTTTTGCCCCAGGCCTGGCCTTGTTGTCAGGCCTTCTTGCCGCCAATCTTGTGCTCGCTGCCGAGACACAAATCGA
CACTGCCAGGGACAGTTTTATTTCCCGCCAGAGTGCACTTGGCTTTTCCCAATCTCAAATCGATGCATTTCTGGCCGGCG
CCCAATATAACCCCAAGGTGATTGAAGCCATCAGCCGCCCCTGGGAAGCCAAACCCTGGCATCTCTACCGACCACGCTTT
CTCACCGAGACGAGGCTCAAGGCAGGGCTCGAGTTCTGGCGAACTCACGAGAGCACCATCGACAGGGCATCGACGCAGTT
TGGCGTTGAGCCGGAGATGATTGTGGCCATTATCGGCATCGAAACCCATTACGGCCAAACCATGGGTAACTACAGTGTGC
GCGATGCCCTCTACACCCTTGGGTTTTACTACCCACCCAGGGCCAGTTTTTTTCTTAAAGAATTGGGCGAGCTTCAAAAA
CTTGAGCAGGAAGAAAAACTCGATGTGAGCGCATTGAAAGGCTCCTACGCCGGTGCCATAGGGTTTGGACAGTTTATGCC
CTCCAGCTACCGCTACTATGGGGTCGATTTTGACGGTGATGGCCGCAAAGACTTGCTCGGCAGCCCCGCCGATGCCATCG
GCAGTGTGGCCAATTATTTTCATCAACATGGCTGGCAAACCGGTGACCCTGTCGCGTTTCGCTTGGAAAGCTACGGAGAA
ACACCGCCGACAGCCTCGCTCTGGCAGGGAGAGCCGTTAACCCTCAAGGCATCCGATATACTCGCAGACGGCTTCGGCCT
TGCGGACAAACGGGATCTGGATGTTGCCCGGCCTGCAATGCTGTTTAAACTGGAGCAGGAAACTCACAACGAATACTGGA
TGGGTTTTAAGAATTTTTTCGTAATCACCCGTTATAATCGCAGCCCTCTTTACGCCATGGCGGCCTATGAATTCAGTGAG
CAGCTTAAACATGCGCATGCCAAGTATTAA

Upstream 100 bases:

>100_bases
TAGCTATGGCGGCACCTCGATGCTGACATTGATGACAGGTTTTGGCATTCTAATGAGCATTCAAACCCACAGGCGCTTTG
TCGACCGCTAAGGAATGCCG

Downstream 100 bases:

>100_bases
ATCCTGTTGGCCGTTATTGGCTCTGACACTCATTCTGGGTGGTTGCTCTTCCTCGCCGGAGCCCGGGAGCAGCACTAAAA
CCAGTACCAAGGGGGACAGG

Product: lytic murein transglycosylase B

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSFAPGLALLSGLLAANLVLAAETQIDTARDSFISRQSALGFSQSQIDAFLAGAQYNPKVIEAISRPWEAKPWHLYRPRF
LTETRLKAGLEFWRTHESTIDRASTQFGVEPEMIVAIIGIETHYGQTMGNYSVRDALYTLGFYYPPRASFFLKELGELQK
LEQEEKLDVSALKGSYAGAIGFGQFMPSSYRYYGVDFDGDGRKDLLGSPADAIGSVANYFHQHGWQTGDPVAFRLESYGE
TPPTASLWQGEPLTLKASDILADGFGLADKRDLDVARPAMLFKLEQETHNEYWMGFKNFFVITRYNRSPLYAMAAYEFSE
QLKHAHAKY

Sequences:

>Translated_329_residues
MSFAPGLALLSGLLAANLVLAAETQIDTARDSFISRQSALGFSQSQIDAFLAGAQYNPKVIEAISRPWEAKPWHLYRPRF
LTETRLKAGLEFWRTHESTIDRASTQFGVEPEMIVAIIGIETHYGQTMGNYSVRDALYTLGFYYPPRASFFLKELGELQK
LEQEEKLDVSALKGSYAGAIGFGQFMPSSYRYYGVDFDGDGRKDLLGSPADAIGSVANYFHQHGWQTGDPVAFRLESYGE
TPPTASLWQGEPLTLKASDILADGFGLADKRDLDVARPAMLFKLEQETHNEYWMGFKNFFVITRYNRSPLYAMAAYEFSE
QLKHAHAKY
>Mature_328_residues
SFAPGLALLSGLLAANLVLAAETQIDTARDSFISRQSALGFSQSQIDAFLAGAQYNPKVIEAISRPWEAKPWHLYRPRFL
TETRLKAGLEFWRTHESTIDRASTQFGVEPEMIVAIIGIETHYGQTMGNYSVRDALYTLGFYYPPRASFFLKELGELQKL
EQEEKLDVSALKGSYAGAIGFGQFMPSSYRYYGVDFDGDGRKDLLGSPADAIGSVANYFHQHGWQTGDPVAFRLESYGET
PPTASLWQGEPLTLKASDILADGFGLADKRDLDVARPAMLFKLEQETHNEYWMGFKNFFVITRYNRSPLYAMAAYEFSEQ
LKHAHAKY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=256, Percent_Identity=43.359375, Blast_Score=201, Evalue=7e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36942; Mature: 36811

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFAPGLALLSGLLAANLVLAAETQIDTARDSFISRQSALGFSQSQIDAFLAGAQYNPKV
CCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
IEAISRPWEAKPWHLYRPRFLTETRLKAGLEFWRTHESTIDRASTQFGVEPEMIVAIIGI
HHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
ETHYGQTMGNYSVRDALYTLGFYYPPRASFFLKELGELQKLEQEEKLDVSALKGSYAGAI
EHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GFGQFMPSSYRYYGVDFDGDGRKDLLGSPADAIGSVANYFHQHGWQTGDPVAFRLESYGE
HHHHCCCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TPPTASLWQGEPLTLKASDILADGFGLADKRDLDVARPAMLFKLEQETHNEYWMGFKNFF
CCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHCHHHHEEEHHHHCCHHHCCEEEEE
VITRYNRSPLYAMAAYEFSEQLKHAHAKY
EEEEECCCCEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFAPGLALLSGLLAANLVLAAETQIDTARDSFISRQSALGFSQSQIDAFLAGAQYNPKV
CCCCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
IEAISRPWEAKPWHLYRPRFLTETRLKAGLEFWRTHESTIDRASTQFGVEPEMIVAIIGI
HHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEE
ETHYGQTMGNYSVRDALYTLGFYYPPRASFFLKELGELQKLEQEEKLDVSALKGSYAGAI
EHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCC
GFGQFMPSSYRYYGVDFDGDGRKDLLGSPADAIGSVANYFHQHGWQTGDPVAFRLESYGE
HHHHCCCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
TPPTASLWQGEPLTLKASDILADGFGLADKRDLDVARPAMLFKLEQETHNEYWMGFKNFF
CCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCHHCHHHHEEEHHHHCCHHHCCEEEEE
VITRYNRSPLYAMAAYEFSEQLKHAHAKY
EEEEECCCCEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]