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The map label for this gene is ubiF [H]

Identifier: 119775705

GI number: 119775705

Start: 3080124

End: 3081392

Strand: Reverse

Name: ubiF [H]

Synonym: Sama_2573

Alternate gene names: 119775705

Gene position: 3081392-3080124 (Counterclockwise)

Preceding gene: 119775713

Following gene: 119775704

Centisome position: 71.56

GC content: 55.48

Gene sequence:

>1269_bases
ATGTGGCCAAACCCAGCGTTAGACACAATTACACACCAATGGGCACAGGTATTTACAAACGGCGGAGGTTTAGCTTCTTT
GACTGACATGATTGAACAGGATGTAGTGGTGGTGGGCGGTGGCATGGTAGGGGCAGCCCTTGCTGCTGGCCTTGGCCGCG
CCGGATTGTCCGTAACAGTGCTTGAAAACCACATGCCACAGGCGTTTGAGGCGAGTCAGCCCCTGGATGTCAGAGTATCG
GCCCTGAGTGTGGCCAGTGAAGCGCTGCTTGAGCGTCTGGATGCCTGGGCAGGGATACTCTCCAGGCGCGCCGTACCTTA
TCTTGGTCTTGAGACCTGGGAAATTGAATCCTGTATCACCCGTTTCCATGCCAGCCAGATAGGCGCTGACCATTTGGGAC
ACATAGTCGAAAACCGGGTGGTGCAGTTGGCGCTTTGGGACCTTTTGTCAACGCTGGATTCAGTTACTGTTAAAGCCCCC
GTTAACGTGATGGGCTTCGAGCGCCTTGTGGATGAGGACGCCATTTGTGTCGCCCTCGACTCCGGCGAGCGTATTAAAGC
AAAGCTCTTGGTGGGCGCAGATGGCGCCAATTCCCAGGTGCGTGCTTTCGCTCATATTGGGGTGACGGGTTGGGACTATG
CCCAGTCGGCCATGCTTATCAATATCGCCACCGCCTGTGAGCAGCAGGATGTGACCTGGCAGCAGTTTACGCCCCAGGGC
CCCCGTTCGCTGCTGCCACTGCCGGGCAAAAACGCGTCTCTGGTATGGTATGACGACGCTTCAACCATTGCCCGGCTCGC
GAAGCTGAACCCTAACGAGCTGGCAGATTCAATCCGGGCCCATTTCCCCGCCCGCCTTGATCGGGATTTTAGAGTGTTGG
ACAAGGGGCACTTTAAGCTGACCCGCCGCCACGCCCAGCGCTATTTCGATGCCAATCTGGTGTTGATTGGCGATGCAGCC
CACACCATTAACCCCTTGGCCGGTCAGGGCGTTAACCTGGGCTTTAAGGATGTTGATGTGCTGCTGGATGAAATTGTCAG
TGCAGTGAAAAAAGGTGAAAACTGGCATACAAAATCAGTTCTGGAGCGTTACCAGCAACGGCGCTGGCGTGACAATCAGC
TGATGATGTCCACCATGGATGCCTTCTATGCCTCGTTCAGCAATGACTTGTTGCCAGTTAAGTTGCTGCGCAATGCGGCG
CTGCGGCTTGCCAATGTGGATGGGCCCATCAAACGTCAGGTGCTGAAATATGCCATGGGGTTGAAATAG

Upstream 100 bases:

>100_bases
CTCGATGCTTTCACAAAATGCCGTTACCGGCGGGAACTCAACTGTGGCTGCTTCAGTGTAGGTGGGACTTTTCGATGGCT
TGTGTGGTCTTTGTAACCAG

Downstream 100 bases:

>100_bases
TACCTTCTGGATCAAACCAAGGCGCACCAAGCGCCTTGGTATTCACCTCACCCACAATCTTGAATTCCATTCCGTTATTG
CCTGTTTTCCTTAGTTGCGC

Product: 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MWPNPALDTITHQWAQVFTNGGGLASLTDMIEQDVVVVGGGMVGAALAAGLGRAGLSVTVLENHMPQAFEASQPLDVRVS
ALSVASEALLERLDAWAGILSRRAVPYLGLETWEIESCITRFHASQIGADHLGHIVENRVVQLALWDLLSTLDSVTVKAP
VNVMGFERLVDEDAICVALDSGERIKAKLLVGADGANSQVRAFAHIGVTGWDYAQSAMLINIATACEQQDVTWQQFTPQG
PRSLLPLPGKNASLVWYDDASTIARLAKLNPNELADSIRAHFPARLDRDFRVLDKGHFKLTRRHAQRYFDANLVLIGDAA
HTINPLAGQGVNLGFKDVDVLLDEIVSAVKKGENWHTKSVLERYQQRRWRDNQLMMSTMDAFYASFSNDLLPVKLLRNAA
LRLANVDGPIKRQVLKYAMGLK

Sequences:

>Translated_422_residues
MWPNPALDTITHQWAQVFTNGGGLASLTDMIEQDVVVVGGGMVGAALAAGLGRAGLSVTVLENHMPQAFEASQPLDVRVS
ALSVASEALLERLDAWAGILSRRAVPYLGLETWEIESCITRFHASQIGADHLGHIVENRVVQLALWDLLSTLDSVTVKAP
VNVMGFERLVDEDAICVALDSGERIKAKLLVGADGANSQVRAFAHIGVTGWDYAQSAMLINIATACEQQDVTWQQFTPQG
PRSLLPLPGKNASLVWYDDASTIARLAKLNPNELADSIRAHFPARLDRDFRVLDKGHFKLTRRHAQRYFDANLVLIGDAA
HTINPLAGQGVNLGFKDVDVLLDEIVSAVKKGENWHTKSVLERYQQRRWRDNQLMMSTMDAFYASFSNDLLPVKLLRNAA
LRLANVDGPIKRQVLKYAMGLK
>Mature_422_residues
MWPNPALDTITHQWAQVFTNGGGLASLTDMIEQDVVVVGGGMVGAALAAGLGRAGLSVTVLENHMPQAFEASQPLDVRVS
ALSVASEALLERLDAWAGILSRRAVPYLGLETWEIESCITRFHASQIGADHLGHIVENRVVQLALWDLLSTLDSVTVKAP
VNVMGFERLVDEDAICVALDSGERIKAKLLVGADGANSQVRAFAHIGVTGWDYAQSAMLINIATACEQQDVTWQQFTPQG
PRSLLPLPGKNASLVWYDDASTIARLAKLNPNELADSIRAHFPARLDRDFRVLDKGHFKLTRRHAQRYFDANLVLIGDAA
HTINPLAGQGVNLGFKDVDVLLDEIVSAVKKGENWHTKSVLERYQQRRWRDNQLMMSTMDAFYASFSNDLLPVKLLRNAA
LRLANVDGPIKRQVLKYAMGLK

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=393, Percent_Identity=30.0254452926209, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI32967307, Length=335, Percent_Identity=31.3432835820896, Blast_Score=145, Evalue=6e-35,
Organism=Escherichia coli, GI1786883, Length=388, Percent_Identity=49.7422680412371, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789273, Length=394, Percent_Identity=38.3248730964467, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789274, Length=403, Percent_Identity=28.287841191067, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25146631, Length=433, Percent_Identity=29.5612009237875, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321694, Length=450, Percent_Identity=26.8888888888889, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19920760, Length=448, Percent_Identity=29.6875, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 46333; Mature: 46333

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWPNPALDTITHQWAQVFTNGGGLASLTDMIEQDVVVVGGGMVGAALAAGLGRAGLSVTV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCEEEE
LENHMPQAFEASQPLDVRVSALSVASEALLERLDAWAGILSRRAVPYLGLETWEIESCIT
ECCCCCCHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RFHASQIGADHLGHIVENRVVQLALWDLLSTLDSVTVKAPVNVMGFERLVDEDAICVALD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHCCCCEEEEEC
SGERIKAKLLVGADGANSQVRAFAHIGVTGWDYAQSAMLINIATACEQQDVTWQQFTPQG
CCCEEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHEEEHHHHHCCCCCCHHHCCCCC
PRSLLPLPGKNASLVWYDDASTIARLAKLNPNELADSIRAHFPARLDRDFRVLDKGHFKL
CCCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCEECCCCHHHH
TRRHAQRYFDANLVLIGDAAHTINPLAGQGVNLGFKDVDVLLDEIVSAVKKGENWHTKSV
HHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
LERYQQRRWRDNQLMMSTMDAFYASFSNDLLPVKLLRNAALRLANVDGPIKRQVLKYAMG
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHC
LK
CC
>Mature Secondary Structure
MWPNPALDTITHQWAQVFTNGGGLASLTDMIEQDVVVVGGGMVGAALAAGLGRAGLSVTV
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHCCCCCCEEEE
LENHMPQAFEASQPLDVRVSALSVASEALLERLDAWAGILSRRAVPYLGLETWEIESCIT
ECCCCCCHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
RFHASQIGADHLGHIVENRVVQLALWDLLSTLDSVTVKAPVNVMGFERLVDEDAICVALD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHCCCCEEEEEC
SGERIKAKLLVGADGANSQVRAFAHIGVTGWDYAQSAMLINIATACEQQDVTWQQFTPQG
CCCEEEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHEEEHHHHHCCCCCCHHHCCCCC
PRSLLPLPGKNASLVWYDDASTIARLAKLNPNELADSIRAHFPARLDRDFRVLDKGHFKL
CCCCCCCCCCCCEEEEECCHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCCEECCCCHHHH
TRRHAQRYFDANLVLIGDAAHTINPLAGQGVNLGFKDVDVLLDEIVSAVKKGENWHTKSV
HHHHHHHHHCCCEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
LERYQQRRWRDNQLMMSTMDAFYASFSNDLLPVKLLRNAALRLANVDGPIKRQVLKYAMG
HHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHC
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]