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The map label for this gene is apbE [H]

Identifier: 119775665

GI number: 119775665

Start: 3042011

End: 3043036

Strand: Reverse

Name: apbE [H]

Synonym: Sama_2533

Alternate gene names: 119775665

Gene position: 3043036-3042011 (Counterclockwise)

Preceding gene: 119775666

Following gene: 119775664

Centisome position: 70.67

GC content: 52.44

Gene sequence:

>1026_bases
ATGAAAAAGACCTTATCTAACTGGCTGGTCCTCGTCGGACTGGCCTTTTTTATTTCAGCTTGTTCTAAAGCACCTGAGGT
TGTCAGCCTGTCTGGCAGCACCATGGGCACCACGTATCACATCAAGGTGGTTCCCAGTGACGCCTTACCCCAAAGCCAGC
TGCTGCAGGCAGAAATTGACCTGGCGTTGGAGCGCGTGAATGACCAGATGTCTACTTACCGTCCCACCTCTGAGCTGAGC
CGCTTCAATCAGTTGCCATTGGAGCAGGGTGTTGAGGTGTCGGATGACACTATCAAGGTGGTCCGAGAGGGAATTCGCTT
AAATGAGCTAACCGACGGCGCGCTGGATATTACTCTGGGCCCACTGGTCAATATCTGGGGGTTTGGACCGGACAAGCGAC
CAACTAAATCCCCCACCGAAGCTGAAATCGCCGACGCCAAGACTCGCACCGGTATTCAGAACATCAGCATTGAAGGTAAC
CGCCTCTTTAAGCGCAACGCTCACCTTTATGTGGATTTGTCATCCATTGCCAAGGGCTATGGCGTGGATGTGATTGCCGA
TTTACTGGATAAGTATCACACCAGCGGTTATCTGGTAGAAATTGGCGGTGAACTGCGTATCAAGGGCACCAAGGGTGATG
GTAGCAGCTGGCGTGTAGCCGTTGAAAAGCCACAGGCTGAAGGCCGTGCGGTGTCTCAGGTAATAGAACCCGGTGATATG
GGTATGGCTACCTCTGGCGATTATCGCAATTACTTCGAAGAAAATGGCAAACGCTTCTCACATCTGATAGACCCAAGGAC
CGGTTATCCCATCGAGCACACCCTCGCATCTGTGACAGTGCTGCATCCAAGCTGTATGACTGCCGACGGCCTGGCGACGG
CCATGATGGTGCTCGGCACGGAAGCGTCATTGATTCTTGCCAAACAGCAGGGACTGGCGATAATGCTGATTGAAAAACAG
GGCGAAGAGTTTGTGGTACACTACAGCGACGCATTTTTGCCCTTCGTTAAGTCCACTCAGGAGTGA

Upstream 100 bases:

>100_bases
CCTGTTGGATGACTTCGGCGGTTAATCGCGTTGGCCCCCGGTATACCGGGGGCCAAGTTTCTTAACAGTGTCTGTAAAGA
ATCGAGAAATGCAGGTCCAA

Downstream 100 bases:

>100_bases
TGTATGAGTACCTTTATCGCAGCTTTTGCGGTGCTTATGGTGTTTTTTCTATTGATGTCAATTGGTTATATCATCAAGAA
AAAGGCCGTAGCCGGTAGTT

Product: thiamin biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKKTLSNWLVLVGLAFFISACSKAPEVVSLSGSTMGTTYHIKVVPSDALPQSQLLQAEIDLALERVNDQMSTYRPTSELS
RFNQLPLEQGVEVSDDTIKVVREGIRLNELTDGALDITLGPLVNIWGFGPDKRPTKSPTEAEIADAKTRTGIQNISIEGN
RLFKRNAHLYVDLSSIAKGYGVDVIADLLDKYHTSGYLVEIGGELRIKGTKGDGSSWRVAVEKPQAEGRAVSQVIEPGDM
GMATSGDYRNYFEENGKRFSHLIDPRTGYPIEHTLASVTVLHPSCMTADGLATAMMVLGTEASLILAKQQGLAIMLIEKQ
GEEFVVHYSDAFLPFVKSTQE

Sequences:

>Translated_341_residues
MKKTLSNWLVLVGLAFFISACSKAPEVVSLSGSTMGTTYHIKVVPSDALPQSQLLQAEIDLALERVNDQMSTYRPTSELS
RFNQLPLEQGVEVSDDTIKVVREGIRLNELTDGALDITLGPLVNIWGFGPDKRPTKSPTEAEIADAKTRTGIQNISIEGN
RLFKRNAHLYVDLSSIAKGYGVDVIADLLDKYHTSGYLVEIGGELRIKGTKGDGSSWRVAVEKPQAEGRAVSQVIEPGDM
GMATSGDYRNYFEENGKRFSHLIDPRTGYPIEHTLASVTVLHPSCMTADGLATAMMVLGTEASLILAKQQGLAIMLIEKQ
GEEFVVHYSDAFLPFVKSTQE
>Mature_341_residues
MKKTLSNWLVLVGLAFFISACSKAPEVVSLSGSTMGTTYHIKVVPSDALPQSQLLQAEIDLALERVNDQMSTYRPTSELS
RFNQLPLEQGVEVSDDTIKVVREGIRLNELTDGALDITLGPLVNIWGFGPDKRPTKSPTEAEIADAKTRTGIQNISIEGN
RLFKRNAHLYVDLSSIAKGYGVDVIADLLDKYHTSGYLVEIGGELRIKGTKGDGSSWRVAVEKPQAEGRAVSQVIEPGDM
GMATSGDYRNYFEENGKRFSHLIDPRTGYPIEHTLASVTVLHPSCMTADGLATAMMVLGTEASLILAKQQGLAIMLIEKQ
GEEFVVHYSDAFLPFVKSTQE

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=342, Percent_Identity=39.4736842105263, Blast_Score=252, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37249; Mature: 37249

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLSNWLVLVGLAFFISACSKAPEVVSLSGSTMGTTYHIKVVPSDALPQSQLLQAEID
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHH
LALERVNDQMSTYRPTSELSRFNQLPLEQGVEVSDDTIKVVREGIRLNELTDGALDITLG
HHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEC
PLVNIWGFGPDKRPTKSPTEAEIADAKTRTGIQNISIEGNRLFKRNAHLYVDLSSIAKGY
CHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEEECCEEEEEHHHHCCCC
GVDVIADLLDKYHTSGYLVEIGGELRIKGTKGDGSSWRVAVEKPQAEGRAVSQVIEPGDM
CHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHCCCCCC
GMATSGDYRNYFEENGKRFSHLIDPRTGYPIEHTLASVTVLHPSCMTADGLATAMMVLGT
CCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHCC
EASLILAKQQGLAIMLIEKQGEEFVVHYSDAFLPFVKSTQE
CCEEEEEECCCCEEEEEECCCCEEEEEECCCHHHHHHCCCC
>Mature Secondary Structure
MKKTLSNWLVLVGLAFFISACSKAPEVVSLSGSTMGTTYHIKVVPSDALPQSQLLQAEID
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHH
LALERVNDQMSTYRPTSELSRFNQLPLEQGVEVSDDTIKVVREGIRLNELTDGALDITLG
HHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEC
PLVNIWGFGPDKRPTKSPTEAEIADAKTRTGIQNISIEGNRLFKRNAHLYVDLSSIAKGY
CHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCEEEEECCEEEEEHHHHCCCC
GVDVIADLLDKYHTSGYLVEIGGELRIKGTKGDGSSWRVAVEKPQAEGRAVSQVIEPGDM
CHHHHHHHHHHHCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHCCCCCC
GMATSGDYRNYFEENGKRFSHLIDPRTGYPIEHTLASVTVLHPSCMTADGLATAMMVLGT
CCCCCCCHHHHHHHCCCCHHHHCCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHCC
EASLILAKQQGLAIMLIEKQGEEFVVHYSDAFLPFVKSTQE
CCEEEEEECCCCEEEEEECCCCEEEEEECCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]