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The map label for this gene is yfiF [H]

Identifier: 119775658

GI number: 119775658

Start: 3034996

End: 3035997

Strand: Reverse

Name: yfiF [H]

Synonym: Sama_2526

Alternate gene names: 119775658

Gene position: 3035997-3034996 (Counterclockwise)

Preceding gene: 119775659

Following gene: 119775652

Centisome position: 70.5

GC content: 58.98

Gene sequence:

>1002_bases
ATGCGACCCGCCAGAGCCGACCGCTATCAAGGCCGCAATCAACAGGAGCGCACTCAAGGCGACAAGGGGAGCACAGTGGA
GCATGCTCAGGGCAGGGAAGACCGCGCGAGAAAAGGCGCTGACGGATGCTCGGGTAAGGGGCGTTTTGACCGCAACACTG
GCTCAGGTCGGACTGAACGACGCGACGCTTATCATCACAGAGAAGCTCCCCGCCGAGAACCAGCGCACAGGGCGACTGGC
GCTCAAGCTGGGTTTGATGGCAAGGATCAGCGCCTGAACGAGACCCGTCTCTATGGCATTAATGCCTGCCGTGAAACCTT
CAAGCTGCGCAGCGATGAGGTGATTAAGCTGTATCTGGATGAGGAGACTGCGCCCAAGTTCGCCGATGTGATGAAATACC
TCGCCAGAAACCGCAAGGCATACCATGTGGTGGATGCAGATGAACTGGAGAAAATCGCCGGCAGTCAACATCACGGCGGT
GTGGTGATGGTAGTGCGTCGTAAGCCAGTGCAGGACATAGCAGGCTATTTGGCAGCCAACACAGCTAAATCGCGGGATTG
TTTACTTGCCCTCGATGGCGTGGGCAATCCCCACAACCTGGGCGCCATTATGCGCAGCTGTGCCCATTTCGGCGTGGCAG
GCATAGTGATGACCGAGGCCGGGTTACTTCAGTCCGGTGCTGCCGCCCGTACGGCGGAAGGGGGCATGGAGCATGTGGAG
GGCATTCGCTGCGACAGGCTGACCGATGCGCTGCAGTTATGCCGCGATGCCGGTTACAGCATCATTACCACCTCCAGCCA
CGGCGGTGAGTCTCTATATCGCAGTAAGCTGCCTGCCAGGGTGGTGGTGGTATTCGGTGAGGAAATGGACGGTGTGTCCA
AGGCGGTGGCGAGCCGCGCCGACATTGGTGTGCAAATACCGGGTACCGGGTTGGTGGAGTCGCTGAATGTGAGTGTGGCG
GCCAGTCTGATTTTGGGTGAATGGTATCGCCAGCAGAGCTGA

Upstream 100 bases:

>100_bases
CAGGCGTCAATCCCCATCGGCCAACGCTTCTTCTCCCCGGGAGAAGCAATCCCACGTCAAGCCGTCATCAGAGCGAAAGC
GCAGCGATACGCAGGATGAT

Downstream 100 bases:

>100_bases
GCCACCCGCAACTCACACTCTGCAAGCAACTCTGTCTTATTATGATAAAAGCGCCACTGGGCGCTTTTATTCTTTTATCT
CAACCTATTCTGGGTAAGGA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRPARADRYQGRNQQERTQGDKGSTVEHAQGREDRARKGADGCSGKGRFDRNTGSGRTERRDAYHHREAPRREPAHRATG
AQAGFDGKDQRLNETRLYGINACRETFKLRSDEVIKLYLDEETAPKFADVMKYLARNRKAYHVVDADELEKIAGSQHHGG
VVMVVRRKPVQDIAGYLAANTAKSRDCLLALDGVGNPHNLGAIMRSCAHFGVAGIVMTEAGLLQSGAAARTAEGGMEHVE
GIRCDRLTDALQLCRDAGYSIITTSSHGGESLYRSKLPARVVVVFGEEMDGVSKAVASRADIGVQIPGTGLVESLNVSVA
ASLILGEWYRQQS

Sequences:

>Translated_333_residues
MRPARADRYQGRNQQERTQGDKGSTVEHAQGREDRARKGADGCSGKGRFDRNTGSGRTERRDAYHHREAPRREPAHRATG
AQAGFDGKDQRLNETRLYGINACRETFKLRSDEVIKLYLDEETAPKFADVMKYLARNRKAYHVVDADELEKIAGSQHHGG
VVMVVRRKPVQDIAGYLAANTAKSRDCLLALDGVGNPHNLGAIMRSCAHFGVAGIVMTEAGLLQSGAAARTAEGGMEHVE
GIRCDRLTDALQLCRDAGYSIITTSSHGGESLYRSKLPARVVVVFGEEMDGVSKAVASRADIGVQIPGTGLVESLNVSVA
ASLILGEWYRQQS
>Mature_333_residues
MRPARADRYQGRNQQERTQGDKGSTVEHAQGREDRARKGADGCSGKGRFDRNTGSGRTERRDAYHHREAPRREPAHRATG
AQAGFDGKDQRLNETRLYGINACRETFKLRSDEVIKLYLDEETAPKFADVMKYLARNRKAYHVVDADELEKIAGSQHHGG
VVMVVRRKPVQDIAGYLAANTAKSRDCLLALDGVGNPHNLGAIMRSCAHFGVAGIVMTEAGLLQSGAAARTAEGGMEHVE
GIRCDRLTDALQLCRDAGYSIITTSSHGGESLYRSKLPARVVVVFGEEMDGVSKAVASRADIGVQIPGTGLVESLNVSVA
ASLILGEWYRQQS

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=330, Percent_Identity=39.6969696969697, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1790623, Length=190, Percent_Identity=33.6842105263158, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 36275; Mature: 36275

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPARADRYQGRNQQERTQGDKGSTVEHAQGREDRARKGADGCSGKGRFDRNTGSGRTER
CCCCCCCHHCCCCHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
RDAYHHREAPRREPAHRATGAQAGFDGKDQRLNETRLYGINACRETFKLRSDEVIKLYLD
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCCEEEEEEC
EETAPKFADVMKYLARNRKAYHVVDADELEKIAGSQHHGGVVMVVRRKPVQDIAGYLAAN
CCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHC
TAKSRDCLLALDGVGNPHNLGAIMRSCAHFGVAGIVMTEAGLLQSGAAARTAEGGMEHVE
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
GIRCDRLTDALQLCRDAGYSIITTSSHGGESLYRSKLPARVVVVFGEEMDGVSKAVASRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
DIGVQIPGTGLVESLNVSVAASLILGEWYRQQS
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPARADRYQGRNQQERTQGDKGSTVEHAQGREDRARKGADGCSGKGRFDRNTGSGRTER
CCCCCCCHHCCCCHHHHHCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
RDAYHHREAPRREPAHRATGAQAGFDGKDQRLNETRLYGINACRETFKLRSDEVIKLYLD
HHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHEEEHHHHHHHHHCCCCCEEEEEEC
EETAPKFADVMKYLARNRKAYHVVDADELEKIAGSQHHGGVVMVVRRKPVQDIAGYLAAN
CCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHC
TAKSRDCLLALDGVGNPHNLGAIMRSCAHFGVAGIVMTEAGLLQSGAAARTAEGGMEHVE
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHCCHHHHH
GIRCDRLTDALQLCRDAGYSIITTSSHGGESLYRSKLPARVVVVFGEEMDGVSKAVASRA
CCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCEEEEEEECCCCCHHHHHHHHHC
DIGVQIPGTGLVESLNVSVAASLILGEWYRQQS
CCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]