Click here to switch to the map view.

The map label for this gene is proB

Identifier: 119775648

GI number: 119775648

Start: 3021760

End: 3022899

Strand: Reverse

Name: proB

Synonym: Sama_2516

Alternate gene names: 119775648

Gene position: 3022899-3021760 (Counterclockwise)

Preceding gene: 119775649

Following gene: 119775647

Centisome position: 70.2

GC content: 57.89

Gene sequence:

>1140_bases
ATGAACTCGAGTGTGCAGGGCTACCGACGGGTAGTGGTGAAACTGGGGACCAGTGTGCTGACATCCGGCAGCAAGTCGTT
GGATAAGGCACATATGGTGGAGCTTGCCAGACAAATGGCAGGACTGATGAAGTCAGGTGTGGAAGTGGTATTGGTGACCT
CGGGTGCCATTGCCGCCGGACGCGAACACCTGGGTTACCCGGTATTGCCCGACACCATGGCCAACAAGCAACTCCTGGCC
GCTGTCGGGCAAAGTCAGCTCATTCTCGCCTGGTCACAACTCTTCAGTATCTACGGCCTGCATGTGGGTCAGTTGCTGCT
GACCCGCGCTGATCTGCACGACCGCGAGCGTTATCTCAATGCCCGCGACACCCTCAATGCGCTGCTGGCGCAAGGGATTG
TGCCCATCATCAACGAAAACGACGCGGTGGCGACCACTGAAATTAAAGTAGGGGACAACGACAACCTGTCTGCCCGCGCG
GCACTCCTTTGCGACGCCGATCTCCTGATGTTGCTCACTGATCAGAAAGGATTATTCGATGCTGATCCCCGCAAAGAGCC
CAATGCACGCTTGATCCCCGAGGTGGAGCACATCGACGACAGCCTGCGTCTGCTTGCTGGCGGCTCTGTCTCAGGCCTTG
GCACCGGTGGTATGGCCACCAAGTTGCAGGCAGCGGATATTGCCCGCCGCGCCGGCGTGGAAGTCATCATTGCCTCGGGT
CATCACCCGGAAGTGATTAAAAAGGCGGTTCACCGCGAGGCCGTGGGTACCCGTTTTCTGGCCATTGAGAGTCCACTGGA
GAGTCGCAAACAATGGATTTTGGCCGGTCCCAAGGCGAAAGGGCGCCTGTTGCTGGACATAGGTGCGGTTGCCGCAGTGA
CTTGCCGGGGCCGCAGCCTGTTATCGAAAGGAATTACCGGGGTCGAAGGCCAGTTCGAGCGTGGAGATACCCTGGAGCTG
GTGAGTCCCGATGGTCGGGTGGTGGCAAGGGGCATGGCACGCTACCAGTCTCAGGCATTGAATCTGATTGCCGGTAAACA
CTCTGATGAGATTGAGCCACTGCTTGGTTATGACTATGGCGATGCCGTGGTGCATCGCAACGATATGGTGGTGTTGGAAA
TGATTGGGGAGGCCAAGTGA

Upstream 100 bases:

>100_bases
TCAACATGAGTGCTTTTTGTGGCAAATATCCATCGGCGCCGTTGTGCAAGGGCGGTTGCTTTGGGTATAAGGTGAGTTCG
GCTTTTTTACATGGATCAAG

Downstream 100 bases:

>100_bases
TGGACGCTCAGGCTTATTTATTGCAGTTGGGTCAGGCCGCCAAGAGCTCAGGTTTTGCACTGGCAAACCTTGGCGTAATG
GCCAAAAATCGCTTGCTGGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNSSVQGYRRVVVKLGTSVLTSGSKSLDKAHMVELARQMAGLMKSGVEVVLVTSGAIAAGREHLGYPVLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLAQGIVPIINENDAVATTEIKVGDNDNLSARA
ALLCDADLLMLLTDQKGLFDADPRKEPNARLIPEVEHIDDSLRLLAGGSVSGLGTGGMATKLQAADIARRAGVEVIIASG
HHPEVIKKAVHREAVGTRFLAIESPLESRKQWILAGPKAKGRLLLDIGAVAAVTCRGRSLLSKGITGVEGQFERGDTLEL
VSPDGRVVARGMARYQSQALNLIAGKHSDEIEPLLGYDYGDAVVHRNDMVVLEMIGEAK

Sequences:

>Translated_379_residues
MNSSVQGYRRVVVKLGTSVLTSGSKSLDKAHMVELARQMAGLMKSGVEVVLVTSGAIAAGREHLGYPVLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLAQGIVPIINENDAVATTEIKVGDNDNLSARA
ALLCDADLLMLLTDQKGLFDADPRKEPNARLIPEVEHIDDSLRLLAGGSVSGLGTGGMATKLQAADIARRAGVEVIIASG
HHPEVIKKAVHREAVGTRFLAIESPLESRKQWILAGPKAKGRLLLDIGAVAAVTCRGRSLLSKGITGVEGQFERGDTLEL
VSPDGRVVARGMARYQSQALNLIAGKHSDEIEPLLGYDYGDAVVHRNDMVVLEMIGEAK
>Mature_379_residues
MNSSVQGYRRVVVKLGTSVLTSGSKSLDKAHMVELARQMAGLMKSGVEVVLVTSGAIAAGREHLGYPVLPDTMANKQLLA
AVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLNARDTLNALLAQGIVPIINENDAVATTEIKVGDNDNLSARA
ALLCDADLLMLLTDQKGLFDADPRKEPNARLIPEVEHIDDSLRLLAGGSVSGLGTGGMATKLQAADIARRAGVEVIIASG
HHPEVIKKAVHREAVGTRFLAIESPLESRKQWILAGPKAKGRLLLDIGAVAAVTCRGRSLLSKGITGVEGQFERGDTLEL
VSPDGRVVARGMARYQSQALNLIAGKHSDEIEPLLGYDYGDAVVHRNDMVVLEMIGEAK

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=290, Percent_Identity=34.8275862068965, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI21361368, Length=292, Percent_Identity=34.5890410958904, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1786437, Length=361, Percent_Identity=63.4349030470914, Blast_Score=447, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17569969, Length=267, Percent_Identity=34.0823970037453, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=267, Percent_Identity=34.0823970037453, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=35.6796116504854, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=411, Percent_Identity=34.5498783454988, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=34.2560553633218, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SHEAM (A1S8L2)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928388.1
- ProteinModelPortal:   A1S8L2
- SMR:   A1S8L2
- STRING:   A1S8L2
- GeneID:   4604763
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2516
- NMPDR:   fig|326297.7.peg.2356
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   A1S8L2
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40563; Mature: 40563

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSVQGYRRVVVKLGTSVLTSGSKSLDKAHMVELARQMAGLMKSGVEVVLVTSGAIAAG
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCC
REHLGYPVLPDTMANKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
ARDTLNALLAQGIVPIINENDAVATTEIKVGDNDNLSARAALLCDADLLMLLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKEPNARLIPEVEHIDDSLRLLAGGSVSGLGTGGMATKLQAADIARRAGVEVIIASG
CCCCCCCCCCCCCCHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
HHPEVIKKAVHREAVGTRFLAIESPLESRKQWILAGPKAKGRLLLDIGAVAAVTCRGRSL
CCHHHHHHHHHHHHHCCEEEEECCCHHHCCCEEEECCCCCCEEEEEECCCHHHHHCCHHH
LSKGITGVEGQFERGDTLELVSPDGRVVARGMARYQSQALNLIAGKHSDEIEPLLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC
DAVVHRNDMVVLEMIGEAK
CEEEECCCEEEEEECCCCC
>Mature Secondary Structure
MNSSVQGYRRVVVKLGTSVLTSGSKSLDKAHMVELARQMAGLMKSGVEVVLVTSGAIAAG
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHCC
REHLGYPVLPDTMANKQLLAAVGQSQLILAWSQLFSIYGLHVGQLLLTRADLHDRERYLN
HHHCCCCCCCCHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
ARDTLNALLAQGIVPIINENDAVATTEIKVGDNDNLSARAALLCDADLLMLLTDQKGLFD
HHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCEEEEEECCCCCCC
ADPRKEPNARLIPEVEHIDDSLRLLAGGSVSGLGTGGMATKLQAADIARRAGVEVIIASG
CCCCCCCCCCCCCCHHHCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECC
HHPEVIKKAVHREAVGTRFLAIESPLESRKQWILAGPKAKGRLLLDIGAVAAVTCRGRSL
CCHHHHHHHHHHHHHCCEEEEECCCHHHCCCEEEECCCCCCEEEEEECCCHHHHHCCHHH
LSKGITGVEGQFERGDTLELVSPDGRVVARGMARYQSQALNLIAGKHSDEIEPLLGYDYG
HHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC
DAVVHRNDMVVLEMIGEAK
CEEEECCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA