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The map label for this gene is oprF [H]

Identifier: 119775600

GI number: 119775600

Start: 2972649

End: 2973212

Strand: Reverse

Name: oprF [H]

Synonym: Sama_2466

Alternate gene names: 119775600

Gene position: 2973212-2972649 (Counterclockwise)

Preceding gene: 119775601

Following gene: 119775599

Centisome position: 69.05

GC content: 52.66

Gene sequence:

>564_bases
ATGAACCCTTGGCTTTGCGTTGGTGTGCTGGCGGGACTCTTGAGTTCGCCTCTGCATGCGGTAAGCGACAAAGATGGAGA
TGGAGTACCTGATTTCAAGGATGCCTGTCCCGGTACGCCGGTGGGCAACACAGTGGAGGCCAACGGGTGTAGTATTCCTG
TCAGTGCGCCTGCTCCTGTGTGTCTGATGACGTCACAGATGAATCCATATCCATCCAGTTGTCACCAAGGCGAAATCCTG
TCGATCTATTTTGATTTCGGATCGGCCTTCGTGCCTTTTGAAGCTGCCGCCAAGCTGGCGTGGTTGGCAAAGAGTATCGA
GACAGACCATGTCTCGTTGCTGCTTCAGGGGCACACCGACGACATAGGTACTGAAGCGGCCAATTATCGCTTGTCAGCCG
AACGCGCTGACAATGTTGCAGCTAGTCTCGCCATGGATTTTGCTATCCCTAAAGCGAGAATAACGACAGCATCCTTTGGG
GAGTCAATGCCCGTTGCCCCCAATAACACCGAGATAGGCCGACAGCAAAACCGCCGTGTCGATATTTTGATACTTGTTGA
ATAG

Upstream 100 bases:

>100_bases
TTGCAAATTAAGCAGTAAAATATCTCTCGTTGTTGATTGGGGTTGATAACCCCGACTGCCGGAATGTCTGGCGACGATTA
ACACATCCCGGGATTTCACT

Downstream 100 bases:

>100_bases
CACAGTGTTTTTAAGTTAAGGGAGTAATTTCATGGATAATATCCAAGGATTAATCGATCAGGCTCCAGAACTGATCATGA
CCTACGGTCTGAAAGTCATT

Product: OmpA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNPWLCVGVLAGLLSSPLHAVSDKDGDGVPDFKDACPGTPVGNTVEANGCSIPVSAPAPVCLMTSQMNPYPSSCHQGEIL
SIYFDFGSAFVPFEAAAKLAWLAKSIETDHVSLLLQGHTDDIGTEAANYRLSAERADNVAASLAMDFAIPKARITTASFG
ESMPVAPNNTEIGRQQNRRVDILILVE

Sequences:

>Translated_187_residues
MNPWLCVGVLAGLLSSPLHAVSDKDGDGVPDFKDACPGTPVGNTVEANGCSIPVSAPAPVCLMTSQMNPYPSSCHQGEIL
SIYFDFGSAFVPFEAAAKLAWLAKSIETDHVSLLLQGHTDDIGTEAANYRLSAERADNVAASLAMDFAIPKARITTASFG
ESMPVAPNNTEIGRQQNRRVDILILVE
>Mature_187_residues
MNPWLCVGVLAGLLSSPLHAVSDKDGDGVPDFKDACPGTPVGNTVEANGCSIPVSAPAPVCLMTSQMNPYPSSCHQGEIL
SIYFDFGSAFVPFEAAAKLAWLAKSIETDHVSLLLQGHTDDIGTEAANYRLSAERADNVAASLAMDFAIPKARITTASFG
ESMPVAPNNTEIGRQQNRRVDILILVE

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=82, Percent_Identity=40.2439024390244, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 19789; Mature: 19789

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPWLCVGVLAGLLSSPLHAVSDKDGDGVPDFKDACPGTPVGNTVEANGCSIPVSAPAPV
CCCEEHHHHHHHHHCCCHHHHCCCCCCCCCCHHHCCCCCCCCCEEECCCCEEECCCCCCE
CLMTSQMNPYPSSCHQGEILSIYFDFGSAFVPFEAAAKLAWLAKSIETDHVSLLLQGHTD
EEEECCCCCCCCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHCCCCEEEEEEECCCC
DIGTEAANYRLSAERADNVAASLAMDFAIPKARITTASFGESMPVAPNNTEIGRQQNRRV
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
DILILVE
EEEEEEC
>Mature Secondary Structure
MNPWLCVGVLAGLLSSPLHAVSDKDGDGVPDFKDACPGTPVGNTVEANGCSIPVSAPAPV
CCCEEHHHHHHHHHCCCHHHHCCCCCCCCCCHHHCCCCCCCCCEEECCCCEEECCCCCCE
CLMTSQMNPYPSSCHQGEILSIYFDFGSAFVPFEAAAKLAWLAKSIETDHVSLLLQGHTD
EEEECCCCCCCCCCCCCCEEEEEEECCCEECCHHHHHHHHHHHHHCCCCEEEEEEECCCC
DIGTEAANYRLSAERADNVAASLAMDFAIPKARITTASFGESMPVAPNNTEIGRQQNRRV
CCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEE
DILILVE
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]