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The map label for this gene is mscS [H]

Identifier: 119775599

GI number: 119775599

Start: 2971790

End: 2972617

Strand: Reverse

Name: mscS [H]

Synonym: Sama_2465

Alternate gene names: 119775599

Gene position: 2972617-2971790 (Counterclockwise)

Preceding gene: 119775600

Following gene: 119775598

Centisome position: 69.03

GC content: 50.72

Gene sequence:

>828_bases
ATGGATAATATCCAAGGATTAATCGATCAGGCTCCAGAACTGATCATGACCTACGGTCTGAAAGTCATTTTTGCCATTAT
CATTTTCCTGGTCGGTAAGTACTTCTCCAATTTGGTGAAGAAGTTAACCACCAAGATGCTGAACAACCGCAAGGTTGACC
CAACAGTAACCTCGTTTGTAGCCAACCTGGCGTGGGCAGTGGTGTTTGTTTTTACCGTTATCGCCACCCTGGGGCAAATC
GGTGTACAGACAGCCTCTCTGGTTGCTGTTATCGGTGCCGCCGGTCTGGCTGTGGGTTTGGCACTGCAGGGCTCTTTGTC
GAACTTTGCTGCCGGTGTACTTATGGTGCTGTTCCGCCCATGCCGCGTGGGTGACTACATCGAAGCGGCCGGCATTGCCG
GTACCGTGGATGAAATCACCATTTTCTCGACCAAGCTGCGTACCCCCGATAACAAGGTTATCGTTGCGCCCAACTCTGCC
ATCATGAATGGCACCATCACCAACTACTCTGCCATGGACACGCGCCGTGTAGACATGGTGATTGGTGTTGCCTATGACGC
CGATATCAAGCTGACCAAGAAAGTCCTGACCGACATTCTGGACAACAATCAGTATGTGCTGAAAGACCCTGCGTATACCG
TTGCTCTGAGCGAACTGGGTGCTTCTTCTATAAACTTTGTGGTGCGTCCCTGGGTTAAGGGTGCCGATTTCTGGGCAGCC
AAGTTTGAACTGACCGAGCAGATCAAAAATGCCCTCGATGCCAACAACATTGGTATCCCATACCCTCAGATGGATATCCA
TGTGAAGGAATTACCTGCGGCCAAGTAA

Upstream 100 bases:

>100_bases
GCCCCCAATAACACCGAGATAGGCCGACAGCAAAACCGCCGTGTCGATATTTTGATACTTGTTGAATAGCACAGTGTTTT
TAAGTTAAGGGAGTAATTTC

Downstream 100 bases:

>100_bases
GCTGCAGCAAGGTAAAAAGCCGGCAATCGCCGGCTTTTTTTATTCGAGGGCTGGCGGTATTGGCCACACTTTGCAACACT
CTGCTATCGGTTAATGACAG

Product: putative small-conductance mechanosensitive channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MDNIQGLIDQAPELIMTYGLKVIFAIIIFLVGKYFSNLVKKLTTKMLNNRKVDPTVTSFVANLAWAVVFVFTVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSA
IMNGTITNYSAMDTRRVDMVIGVAYDADIKLTKKVLTDILDNNQYVLKDPAYTVALSELGASSINFVVRPWVKGADFWAA
KFELTEQIKNALDANNIGIPYPQMDIHVKELPAAK

Sequences:

>Translated_275_residues
MDNIQGLIDQAPELIMTYGLKVIFAIIIFLVGKYFSNLVKKLTTKMLNNRKVDPTVTSFVANLAWAVVFVFTVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSA
IMNGTITNYSAMDTRRVDMVIGVAYDADIKLTKKVLTDILDNNQYVLKDPAYTVALSELGASSINFVVRPWVKGADFWAA
KFELTEQIKNALDANNIGIPYPQMDIHVKELPAAK
>Mature_275_residues
MDNIQGLIDQAPELIMTYGLKVIFAIIIFLVGKYFSNLVKKLTTKMLNNRKVDPTVTSFVANLAWAVVFVFTVIATLGQI
GVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRPCRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSA
IMNGTITNYSAMDTRRVDMVIGVAYDADIKLTKKVLTDILDNNQYVLKDPAYTVALSELGASSINFVVRPWVKGADFWAA
KFELTEQIKNALDANNIGIPYPQMDIHVKELPAAK

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=262, Percent_Identity=48.0916030534351, Blast_Score=258, Evalue=3e-70,
Organism=Escherichia coli, GI1786670, Length=253, Percent_Identity=28.0632411067194, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787591, Length=266, Percent_Identity=24.812030075188, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367355, Length=271, Percent_Identity=23.2472324723247, Blast_Score=81, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 29703; Mature: 29703

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNIQGLIDQAPELIMTYGLKVIFAIIIFLVGKYFSNLVKKLTTKMLNNRKVDPTVTSFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ANLAWAVVFVFTVIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSAIMNGTITNYSAMDTRRVDMV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEE
IGVAYDADIKLTKKVLTDILDNNQYVLKDPAYTVALSELGASSINFVVRPWVKGADFWAA
EEEEECCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHCCCCCCCEEEECCCCCCCHHHH
KFELTEQIKNALDANNIGIPYPQMDIHVKELPAAK
HHHHHHHHHHHHCCCCCCCCCCCCCEEHHCCCCCC
>Mature Secondary Structure
MDNIQGLIDQAPELIMTYGLKVIFAIIIFLVGKYFSNLVKKLTTKMLNNRKVDPTVTSFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ANLAWAVVFVFTVIATLGQIGVQTASLVAVIGAAGLAVGLALQGSLSNFAAGVLMVLFRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
CRVGDYIEAAGIAGTVDEITIFSTKLRTPDNKVIVAPNSAIMNGTITNYSAMDTRRVDMV
CCCCCHHHHCCCCCCHHHEEEEEEEECCCCCEEEECCCCEEECCCCCCCCCCCCEEEEEE
IGVAYDADIKLTKKVLTDILDNNQYVLKDPAYTVALSELGASSINFVVRPWVKGADFWAA
EEEEECCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHCCCCCCCEEEECCCCCCCHHHH
KFELTEQIKNALDANNIGIPYPQMDIHVKELPAAK
HHHHHHHHHHHHCCCCCCCCCCCCCEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]