Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 119775592

GI number: 119775592

Start: 2966439

End: 2967767

Strand: Reverse

Name: puuB [H]

Synonym: Sama_2458

Alternate gene names: 119775592

Gene position: 2967767-2966439 (Counterclockwise)

Preceding gene: 119775593

Following gene: 119775588

Centisome position: 68.92

GC content: 57.04

Gene sequence:

>1329_bases
TTGTACGACCCATTAATCAGCACCAATGCGGCGCAGCAAGCCTTGCCGTCCACTTTTTGGTCCGGCACCACTGAACCCTT
ACCTGAGTCACCAAGGCTACAGGGACACCTCCAAACCGATGTGATAGTGATTGGCGGCGGTTTCACAGGACTGCTCACCG
CCTATTATCTGGCGACCGAATATGGCAGGCAGGTCACCTTACTCGAAGCCAACCGAATTGGATTTGGGGCCAGCGCCCGT
AATGGTGGCTTTGTACTTAAGGGCTCGGGTCGCCTGGGGTATGGTCAAATGGCCAAGCGGTGGGATTTGGACACGGCAAA
GGGGATTTATCAGGAGTTCTCCGAGGCGGTTGCCCGGGTGGAAGGGCTTATCCAGGATGGCAAGATAGACTGTAATCCGC
AGGAAAAGGGGTATCTTAAAATTGCCCATAGCCCCAAGGCCATGAAAACACTGCAGGCCGGGGCCGATTTTATTGCCCGC
CACATGGGGCAGGGCGCCGAATTTATCTGCCCGGCGAGACTGAGAATTGATTATCTCAATCACCATCAGTCCTATGGCGC
GCTGCGTCTCGCCGATGGATTTGGCCTGAATCCGCTGAAATTGCTGCAGGGCTATGCCCGTCTGGCCCGAGAAGCTGGCG
TACAAATCTTTGAGCAAAGTACCGTGCTTGCCAGTGTGCGCACTGCCAAAGGTTTCAGCCTGGAGACTGAAGCGGGGCGC
ATTGACGCCAATGCCATGGTGTTTGCTGGCAATGCCTATACTCAGCCCGGTTTGACCGAGACTCTCACGGGGCGCTTTTT
GCCCATTTTGAGCAACGTGATAGTGACTGAGCCCCTGTCTGAAGCCGTGCTGGCCGAGGCCGGGCTATACAGCCGCCAGG
TGGCCATGGATACCCGCATCCTTAAATACTATTACCGCTTGTTACCGGAAAACCGGTTGCTCTTTGGTGGGCGAGGCGCC
GTATGGGGCCGTGACGCCGACAATTCTGTGTACCCGGCGCGTTTGAAAGAGGCCATGAATAAGGCGTTTCCGGCCCTGAC
AGATGTGGGTTTAAGCCACACTTGGCACGGCTGGCTGGCGGCATCCCTCGATGATATGCCCCATGTGTATACCCGAGGTG
GCGAAGGCTACAGCCTGGGATATTGCGGCGCAGGTGTATCTTTCAGCAGCCAGGCGGCCTGGCGGCTTGCGGGCATGTTA
AGTGGTAAGGCGGCGCCGTCATTGCCGCTTTATGGCACGCCATTGCCAGCTTTTCCCCTGGCGCCGCTCAGGCGTGTGGG
GCAGTGGGCCTTTTATCATTACGGCCGCTTTATCGATGCTTGGAGCTGA

Upstream 100 bases:

>100_bases
GAATCAATCCCACGATAATCCGGATTACCTGTGTACCCTGTGTGGCAACGATTGCCATTGCACGGCGTGCGAGATGTTAT
TGGTTTTTCAGGAGTCTTAA

Downstream 100 bases:

>100_bases
CCCGCAGCAGAGCCTGTTCCCGGTAAGCACAAACAAAAAACGCCCTTAAGAGGGCGTTTTTGCTGCTGTCGATTTATAAG
CCTGTCGATAATCAGGCTTC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MYDPLISTNAAQQALPSTFWSGTTEPLPESPRLQGHLQTDVIVIGGGFTGLLTAYYLATEYGRQVTLLEANRIGFGASAR
NGGFVLKGSGRLGYGQMAKRWDLDTAKGIYQEFSEAVARVEGLIQDGKIDCNPQEKGYLKIAHSPKAMKTLQAGADFIAR
HMGQGAEFICPARLRIDYLNHHQSYGALRLADGFGLNPLKLLQGYARLAREAGVQIFEQSTVLASVRTAKGFSLETEAGR
IDANAMVFAGNAYTQPGLTETLTGRFLPILSNVIVTEPLSEAVLAEAGLYSRQVAMDTRILKYYYRLLPENRLLFGGRGA
VWGRDADNSVYPARLKEAMNKAFPALTDVGLSHTWHGWLAASLDDMPHVYTRGGEGYSLGYCGAGVSFSSQAAWRLAGML
SGKAAPSLPLYGTPLPAFPLAPLRRVGQWAFYHYGRFIDAWS

Sequences:

>Translated_442_residues
MYDPLISTNAAQQALPSTFWSGTTEPLPESPRLQGHLQTDVIVIGGGFTGLLTAYYLATEYGRQVTLLEANRIGFGASAR
NGGFVLKGSGRLGYGQMAKRWDLDTAKGIYQEFSEAVARVEGLIQDGKIDCNPQEKGYLKIAHSPKAMKTLQAGADFIAR
HMGQGAEFICPARLRIDYLNHHQSYGALRLADGFGLNPLKLLQGYARLAREAGVQIFEQSTVLASVRTAKGFSLETEAGR
IDANAMVFAGNAYTQPGLTETLTGRFLPILSNVIVTEPLSEAVLAEAGLYSRQVAMDTRILKYYYRLLPENRLLFGGRGA
VWGRDADNSVYPARLKEAMNKAFPALTDVGLSHTWHGWLAASLDDMPHVYTRGGEGYSLGYCGAGVSFSSQAAWRLAGML
SGKAAPSLPLYGTPLPAFPLAPLRRVGQWAFYHYGRFIDAWS
>Mature_442_residues
MYDPLISTNAAQQALPSTFWSGTTEPLPESPRLQGHLQTDVIVIGGGFTGLLTAYYLATEYGRQVTLLEANRIGFGASAR
NGGFVLKGSGRLGYGQMAKRWDLDTAKGIYQEFSEAVARVEGLIQDGKIDCNPQEKGYLKIAHSPKAMKTLQAGADFIAR
HMGQGAEFICPARLRIDYLNHHQSYGALRLADGFGLNPLKLLQGYARLAREAGVQIFEQSTVLASVRTAKGFSLETEAGR
IDANAMVFAGNAYTQPGLTETLTGRFLPILSNVIVTEPLSEAVLAEAGLYSRQVAMDTRILKYYYRLLPENRLLFGGRGA
VWGRDADNSVYPARLKEAMNKAFPALTDVGLSHTWHGWLAASLDDMPHVYTRGGEGYSLGYCGAGVSFSSQAAWRLAGML
SGKAAPSLPLYGTPLPAFPLAPLRRVGQWAFYHYGRFIDAWS

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=415, Percent_Identity=32.289156626506, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 48132; Mature: 48132

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDPLISTNAAQQALPSTFWSGTTEPLPESPRLQGHLQTDVIVIGGGFTGLLTAYYLATE
CCCCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
YGRQVTLLEANRIGFGASARNGGFVLKGSGRLGYGQMAKRWDLDTAKGIYQEFSEAVARV
HCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
EGLIQDGKIDCNPQEKGYLKIAHSPKAMKTLQAGADFIARHMGQGAEFICPARLRIDYLN
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEHHHH
HHQSYGALRLADGFGLNPLKLLQGYARLAREAGVQIFEQSTVLASVRTAKGFSLETEAGR
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCE
IDANAMVFAGNAYTQPGLTETLTGRFLPILSNVIVTEPLSEAVLAEAGLYSRQVAMDTRI
ECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
LKYYYRLLPENRLLFGGRGAVWGRDADNSVYPARLKEAMNKAFPALTDVGLSHTWHGWLA
HHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHC
ASLDDMPHVYTRGGEGYSLGYCGAGVSFSSQAAWRLAGMLSGKAAPSLPLYGTPLPAFPL
CCCCCCCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
APLRRVGQWAFYHYGRFIDAWS
HHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure
MYDPLISTNAAQQALPSTFWSGTTEPLPESPRLQGHLQTDVIVIGGGFTGLLTAYYLATE
CCCCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHH
YGRQVTLLEANRIGFGASARNGGFVLKGSGRLGYGQMAKRWDLDTAKGIYQEFSEAVARV
HCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
EGLIQDGKIDCNPQEKGYLKIAHSPKAMKTLQAGADFIARHMGQGAEFICPARLRIDYLN
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEHHHH
HHQSYGALRLADGFGLNPLKLLQGYARLAREAGVQIFEQSTVLASVRTAKGFSLETEAGR
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEECCCCE
IDANAMVFAGNAYTQPGLTETLTGRFLPILSNVIVTEPLSEAVLAEAGLYSRQVAMDTRI
ECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHH
LKYYYRLLPENRLLFGGRGAVWGRDADNSVYPARLKEAMNKAFPALTDVGLSHTWHGWLA
HHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHC
ASLDDMPHVYTRGGEGYSLGYCGAGVSFSSQAAWRLAGMLSGKAAPSLPLYGTPLPAFPL
CCCCCCCCCEECCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
APLRRVGQWAFYHYGRFIDAWS
HHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]