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The map label for this gene is nemA [H]

Identifier: 119775541

GI number: 119775541

Start: 2894172

End: 2895215

Strand: Reverse

Name: nemA [H]

Synonym: Sama_2407

Alternate gene names: 119775541

Gene position: 2895215-2894172 (Counterclockwise)

Preceding gene: 119775543

Following gene: 119775539

Centisome position: 67.23

GC content: 55.94

Gene sequence:

>1044_bases
ATGACTGACATACTCTTTACTCCAGTGGCGCTGAATGAGCGCATCGAGCTGAAAAACCGCATCCTGATGGCGCCTCTGAC
CCGTTGTATGGCAGACGATGCGCTGGTGCCTACCCAGGCTATGGTCGACTACTATGCCCGCCGGGCAGATGCAGGGCTGA
TTATCAGTGAGGCCACCATTATTCGCCCCGATGGTCAGGGTTATCCCAACACCCCGGGTATTTTCACCCAGGCGCAAATT
GCCGGATGGCGTAAGGTCACCGATGCCGTACATGAAAATGGCGGCAAAATTTTTGCCCAACTGTGGCACACAGGCCGGGT
GGCACATCCTCACTTTTGGGGTGGCACAGAAGTGATTGCGCCGTCGGCGGTGGGGGTAGAGGGTACAGTGCCCAGAATGC
GTGAGCTCACCTATTTGGTTCCCCGTGAAGCCACAGCGCTGGAAATTGCCCAGTTGGTGGAGGACTATGCCAAGGCGGCT
GAAAATGCCATCGAAGCCGGGTTCGACGGCGTCGAAATCCACGGTGCCAACGGTTATATGATTGACGAATTCCTGCATTA
CGACAGCAATCGCCGCGAAGATGAGTTTGGTGGCAGCCCCGAAAACATGGTGTGCTTCCCGCTGCAGGTTGTGGATGCCG
TTACTTCACGCATCGGTGCTGCGCGCACGGCACTGCGTATTTCACCGGCGGCTTACTTCAATATCGAGCCGGATCCCCGC
GACCGGGCGGTGTTTGATCTTTTGCTGTCTGAATTGGATACCCGTGAACTTGCCTATTTGCACGGCGGTATATTCGACGA
CAGCGTTCATTATGACTACCTCGACGGCCGGGTCAGTGAGTACCTGCGTCGCCACAGCCGTCATACCCTGGTGGGAGTGG
GTAGCTACGATGCCGAAACAGCCGCTGCTGCCATTGCCACCAATAAGTTCGACGTGATTGCGATTGGCAGACCTTTTATT
GCCAATCACGATTATGTTGCACGGGTACGTGAAGGCGCAGAGCTCAAGCCCTACAGTGATGAGCTGCTGCCAGTGCTTTA
CTGA

Upstream 100 bases:

>100_bases
AGGGTAACAGACTTTTTTGAATGTATGTTCAAAAAAGTTCTTGAATGTATGTTCAAGAATGATTATTTTGAATGCCTGTT
CAAAATAGGAACCCCCATTC

Downstream 100 bases:

>100_bases
TCCGCTTGGTGATACCAATAAAAAAGCCCCGCAGCAGCGGGGCTTTTTTCTGTCAGGGGCGCATCAATCGTTGTCGAGTT
TATGTTTGCCCAGCACAGTG

Product: Oye family NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTDILFTPVALNERIELKNRILMAPLTRCMADDALVPTQAMVDYYARRADAGLIISEATIIRPDGQGYPNTPGIFTQAQI
AGWRKVTDAVHENGGKIFAQLWHTGRVAHPHFWGGTEVIAPSAVGVEGTVPRMRELTYLVPREATALEIAQLVEDYAKAA
ENAIEAGFDGVEIHGANGYMIDEFLHYDSNRREDEFGGSPENMVCFPLQVVDAVTSRIGAARTALRISPAAYFNIEPDPR
DRAVFDLLLSELDTRELAYLHGGIFDDSVHYDYLDGRVSEYLRRHSRHTLVGVGSYDAETAAAAIATNKFDVIAIGRPFI
ANHDYVARVREGAELKPYSDELLPVLY

Sequences:

>Translated_347_residues
MTDILFTPVALNERIELKNRILMAPLTRCMADDALVPTQAMVDYYARRADAGLIISEATIIRPDGQGYPNTPGIFTQAQI
AGWRKVTDAVHENGGKIFAQLWHTGRVAHPHFWGGTEVIAPSAVGVEGTVPRMRELTYLVPREATALEIAQLVEDYAKAA
ENAIEAGFDGVEIHGANGYMIDEFLHYDSNRREDEFGGSPENMVCFPLQVVDAVTSRIGAARTALRISPAAYFNIEPDPR
DRAVFDLLLSELDTRELAYLHGGIFDDSVHYDYLDGRVSEYLRRHSRHTLVGVGSYDAETAAAAIATNKFDVIAIGRPFI
ANHDYVARVREGAELKPYSDELLPVLY
>Mature_346_residues
TDILFTPVALNERIELKNRILMAPLTRCMADDALVPTQAMVDYYARRADAGLIISEATIIRPDGQGYPNTPGIFTQAQIA
GWRKVTDAVHENGGKIFAQLWHTGRVAHPHFWGGTEVIAPSAVGVEGTVPRMRELTYLVPREATALEIAQLVEDYAKAAE
NAIEAGFDGVEIHGANGYMIDEFLHYDSNRREDEFGGSPENMVCFPLQVVDAVTSRIGAARTALRISPAAYFNIEPDPRD
RAVFDLLLSELDTRELAYLHGGIFDDSVHYDYLDGRVSEYLRRHSRHTLVGVGSYDAETAAAAIATNKFDVIAIGRPFIA
NHDYVARVREGAELKPYSDELLPVLY

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=350, Percent_Identity=40, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1789463, Length=220, Percent_Identity=36.8181818181818, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=376, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI72001454, Length=358, Percent_Identity=25.6983240223464, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=377, Percent_Identity=23.8726790450928, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=342, Percent_Identity=26.6081871345029, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=349, Percent_Identity=23.4957020057307, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540738, Length=220, Percent_Identity=25.9090909090909, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=358, Percent_Identity=33.5195530726257, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325086, Length=357, Percent_Identity=33.0532212885154, Blast_Score=159, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38424; Mature: 38293

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDILFTPVALNERIELKNRILMAPLTRCMADDALVPTQAMVDYYARRADAGLIISEATI
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECEEE
IRPDGQGYPNTPGIFTQAQIAGWRKVTDAVHENGGKIFAQLWHTGRVAHPHFWGGTEVIA
ECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCEEC
PSAVGVEGTVPRMRELTYLVPREATALEIAQLVEDYAKAAENAIEAGFDGVEIHGANGYM
CCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
IDEFLHYDSNRREDEFGGSPENMVCFPLQVVDAVTSRIGAARTALRISPAAYFNIEPDPR
HHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCC
DRAVFDLLLSELDTRELAYLHGGIFDDSVHYDYLDGRVSEYLRRHSRHTLVGVGSYDAET
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHCCCCEEEEECCCCCHH
AAAAIATNKFDVIAIGRPFIANHDYVARVREGAELKPYSDELLPVLY
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEECC
>Mature Secondary Structure 
TDILFTPVALNERIELKNRILMAPLTRCMADDALVPTQAMVDYYARRADAGLIISEATI
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEECEEE
IRPDGQGYPNTPGIFTQAQIAGWRKVTDAVHENGGKIFAQLWHTGRVAHPHFWGGTEVIA
ECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCEEC
PSAVGVEGTVPRMRELTYLVPREATALEIAQLVEDYAKAAENAIEAGFDGVEIHGANGYM
CCCCCCCCCCHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
IDEFLHYDSNRREDEFGGSPENMVCFPLQVVDAVTSRIGAARTALRISPAAYFNIEPDPR
HHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHEEECCEEEEECCCCCC
DRAVFDLLLSELDTRELAYLHGGIFDDSVHYDYLDGRVSEYLRRHSRHTLVGVGSYDAET
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHCHHHHHHHHHCCCCEEEEECCCCCHH
AAAAIATNKFDVIAIGRPFIANHDYVARVREGAELKPYSDELLPVLY
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]