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The map label for this gene is fixJ [H]

Identifier: 119775533

GI number: 119775533

Start: 2882241

End: 2882846

Strand: Reverse

Name: fixJ [H]

Synonym: Sama_2399

Alternate gene names: 119775533

Gene position: 2882846-2882241 (Counterclockwise)

Preceding gene: 119775534

Following gene: 119775532

Centisome position: 66.95

GC content: 56.27

Gene sequence:

>606_bases
ATGATTGATGTGTATCTGGTGGATGACGACCCCGATGTATTGGCGTCACTGAAATGGATGCTCGAAGGCCTTGGCATCAA
AGCGCGGGCCTTTGCATCGGCAGAGGACTTTCTGTCCACTGTCGACTTGGCCCGTCCCGCTGTTGCCGTACTGGACGTGC
AGATGCCCGATATGGACGGGATGAGCCTGCTGGAGGTGCTGTCCTCCCGGCCAAGTCCGCTGGTGCCGTTAATGCTCACC
GGCCATGGCAATATCTCCATGGCCGTGAAAGCAATACAACAGGGCGCCATGGATTTTATCGAAAAGCCGGTGGATGGTGA
CAAACTGCTGGCGTTGCTGCGCCAGGCCGCCAGGGTTGCCACAGAGCGTGCAGTCAGCCTGCAGCAGTCGGCCGACATCC
AGCAGCGGCTCGATAGCCTGTCCCCAAGAGAAACCGACGTGATGCACAGGGTGCTTGAGGGCAAACTCAATAAAACCATT
GCCGCAGAATTATTTATCACCCAAAGAACGGTTGAGATCCACCGCCGCAACCTGCTGGAAAAAATGGCCGCAAGCAATGT
GGCCGAGCTGGCTTTTGTATTGTCAAAGCTGCATCAGCAGCGTTAG

Upstream 100 bases:

>100_bases
CGTTTTCATCACTCCGCAATCGAGGGCCAAATCTATGCCCCCATTCACAGCAGCCCCTGTGGGCTGGAAATATGCATAGA
GATGCCCCGGGGAGCCGCTC

Downstream 100 bases:

>100_bases
ACCCAGGCTTTGAAGCCGCATGCAGGCAAAGACGCCTAAGCTCGCAGTTTGCCAACTCAAGTCCGGCAGCAGTAACCAGC
AGCCCCTATTGAGCCCGGCT

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIDVYLVDDDPDVLASLKWMLEGLGIKARAFASAEDFLSTVDLARPAVAVLDVQMPDMDGMSLLEVLSSRPSPLVPLMLT
GHGNISMAVKAIQQGAMDFIEKPVDGDKLLALLRQAARVATERAVSLQQSADIQQRLDSLSPRETDVMHRVLEGKLNKTI
AAELFITQRTVEIHRRNLLEKMAASNVAELAFVLSKLHQQR

Sequences:

>Translated_201_residues
MIDVYLVDDDPDVLASLKWMLEGLGIKARAFASAEDFLSTVDLARPAVAVLDVQMPDMDGMSLLEVLSSRPSPLVPLMLT
GHGNISMAVKAIQQGAMDFIEKPVDGDKLLALLRQAARVATERAVSLQQSADIQQRLDSLSPRETDVMHRVLEGKLNKTI
AAELFITQRTVEIHRRNLLEKMAASNVAELAFVLSKLHQQR
>Mature_201_residues
MIDVYLVDDDPDVLASLKWMLEGLGIKARAFASAEDFLSTVDLARPAVAVLDVQMPDMDGMSLLEVLSSRPSPLVPLMLT
GHGNISMAVKAIQQGAMDFIEKPVDGDKLLALLRQAARVATERAVSLQQSADIQQRLDSLSPRETDVMHRVLEGKLNKTI
AAELFITQRTVEIHRRNLLEKMAASNVAELAFVLSKLHQQR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=31.5, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790299, Length=150, Percent_Identity=34.6666666666667, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788712, Length=191, Percent_Identity=26.1780104712042, Blast_Score=78, Evalue=3e-16,
Organism=Escherichia coli, GI1788521, Length=189, Percent_Identity=31.2169312169312, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=32.7586206896552, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=23.469387755102, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22155; Mature: 22155

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYLVDDDPDVLASLKWMLEGLGIKARAFASAEDFLSTVDLARPAVAVLDVQMPDMDG
CEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCC
MSLLEVLSSRPSPLVPLMLTGHGNISMAVKAIQQGAMDFIEKPVDGDKLLALLRQAARVA
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TERAVSLQQSADIQQRLDSLSPRETDVMHRVLEGKLNKTIAAELFITQRTVEIHRRNLLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMAASNVAELAFVLSKLHQQR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDVYLVDDDPDVLASLKWMLEGLGIKARAFASAEDFLSTVDLARPAVAVLDVQMPDMDG
CEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEECCCCCC
MSLLEVLSSRPSPLVPLMLTGHGNISMAVKAIQQGAMDFIEKPVDGDKLLALLRQAARVA
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TERAVSLQQSADIQQRLDSLSPRETDVMHRVLEGKLNKTIAAELFITQRTVEIHRRNLLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMAASNVAELAFVLSKLHQQR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]