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The map label for this gene is iadA [H]

Identifier: 119775525

GI number: 119775525

Start: 2871106

End: 2872278

Strand: Reverse

Name: iadA [H]

Synonym: Sama_2391

Alternate gene names: 119775525

Gene position: 2872278-2871106 (Counterclockwise)

Preceding gene: 119775526

Following gene: 119775523

Centisome position: 66.7

GC content: 55.92

Gene sequence:

>1173_bases
ATGTTAACTTTGATCAGAAATGCCAACCTTTTTACGCCTTCAGCGGTTGGTTTACAGGATGTGCTTTTTGGCACTGGTGG
CGTTATCACCATCGCAGAGCCGGGCTCGCTGCATGTCGGTTCCTTGCCATGCCATGAAGTCGATGCCAAAGGCAGTGCAC
TGGTACCTGGGCTCGTTGATCCGTTGGTACACATCAGTGGCGGCGGCGGAGAAGGCGGCTTTCATACCCGCACCCCTGAG
ATGCAATTGACTGAGGCCACACTGGCAGGAGTCACAACCCTGGTGGGGGCACTGGGGACAGATGCCGTCAGTCGCAGCCT
GGAAGACTTGCTTGCCAAGGCGCGCGGTTTGGCGAATGAAGGCTTAACCACCTATATCTACACCGGCTCTTACGAGTATC
CGGTCAAAACCCTCACAGGGGACATTACCCGGGACATGATGCTGATTGACAAGGTAATTGGCGTAGGTGAAATCGCCATT
GCCGATCATCGTGGCTCGCAGCTTTCTGCCGCTGAACTGGCCCGCGTTGCCAGCCAGGCACGGGTGGGAGGCATGTTAAG
CGGAAAGGGCGGCATAGTGTTTGTACATGTGGGGGATGGTAAAGGCGGGCTGGATATCCTGTCTGAGGTTGCCGGCACCA
CCGATATTCCGCTGTCGCAATTCTATCCTACCCACATTAATCGTTCCCGTGCATTGCTCGAGCAGGGCGTTGCCTTTGCC
CTGGCCGGTGGCGTGATTGATTTCACGGCCTCTGAAAACAGCGCCACCTTAAACAGTGACGAAATCAGTGCGATAGATGC
GGTAAAGCATGCCCTGGCGGCTGGCGTGGCGGTATCACAAATTACCTTAAGCTCCGATGGCAATGCCAGTTTGCCGGTAT
TCGATGCCGATGGTCGCTTAACCGGCCTCGAAGTGGGTCGGGTCGGCAGTCTGGCAAAGGCATTTCGTGACCTTATCAGC
GAAGGTATTGCCGTGGCCGATGCCCTTGCCATGACCTCCTCCAACGCAGCCCGAATATTGGGGCTTGATACCAAAGGGGC
CATCGCTGAGGGGAAAGACGCCGATTTGGTGCTGCTGAATGACCAGTTCGAGCCTGAATTTGTGTGGTGCCTTGGCAAAG
CCATGGTTGCCAATGGCCTGCCACTGGTTCGGGGAACCTTTGAGATAGTCTGA

Upstream 100 bases:

>100_bases
CCTTTGGACAGTGGCTTAAATTGATTTGGCGATTACAGGTGCTGCTGATGACTCTGGCCAGTGCTGCTGTGGGTATTGCC
GTTTATACAGGATATAGCTG

Downstream 100 bases:

>100_bases
GCAAAGGGGTTACTGCCCCAATGTAAAAAGGGAGCATAGCTCCCTTTCTATTTGTTCAGTCTGTGACTGCGGCTTTGTTC
ATATCATGCTACCTGTGTGC

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MLTLIRNANLFTPSAVGLQDVLFGTGGVITIAEPGSLHVGSLPCHEVDAKGSALVPGLVDPLVHISGGGGEGGFHTRTPE
MQLTEATLAGVTTLVGALGTDAVSRSLEDLLAKARGLANEGLTTYIYTGSYEYPVKTLTGDITRDMMLIDKVIGVGEIAI
ADHRGSQLSAAELARVASQARVGGMLSGKGGIVFVHVGDGKGGLDILSEVAGTTDIPLSQFYPTHINRSRALLEQGVAFA
LAGGVIDFTASENSATLNSDEISAIDAVKHALAAGVAVSQITLSSDGNASLPVFDADGRLTGLEVGRVGSLAKAFRDLIS
EGIAVADALAMTSSNAARILGLDTKGAIAEGKDADLVLLNDQFEPEFVWCLGKAMVANGLPLVRGTFEIV

Sequences:

>Translated_390_residues
MLTLIRNANLFTPSAVGLQDVLFGTGGVITIAEPGSLHVGSLPCHEVDAKGSALVPGLVDPLVHISGGGGEGGFHTRTPE
MQLTEATLAGVTTLVGALGTDAVSRSLEDLLAKARGLANEGLTTYIYTGSYEYPVKTLTGDITRDMMLIDKVIGVGEIAI
ADHRGSQLSAAELARVASQARVGGMLSGKGGIVFVHVGDGKGGLDILSEVAGTTDIPLSQFYPTHINRSRALLEQGVAFA
LAGGVIDFTASENSATLNSDEISAIDAVKHALAAGVAVSQITLSSDGNASLPVFDADGRLTGLEVGRVGSLAKAFRDLIS
EGIAVADALAMTSSNAARILGLDTKGAIAEGKDADLVLLNDQFEPEFVWCLGKAMVANGLPLVRGTFEIV
>Mature_390_residues
MLTLIRNANLFTPSAVGLQDVLFGTGGVITIAEPGSLHVGSLPCHEVDAKGSALVPGLVDPLVHISGGGGEGGFHTRTPE
MQLTEATLAGVTTLVGALGTDAVSRSLEDLLAKARGLANEGLTTYIYTGSYEYPVKTLTGDITRDMMLIDKVIGVGEIAI
ADHRGSQLSAAELARVASQARVGGMLSGKGGIVFVHVGDGKGGLDILSEVAGTTDIPLSQFYPTHINRSRALLEQGVAFA
LAGGVIDFTASENSATLNSDEISAIDAVKHALAAGVAVSQITLSSDGNASLPVFDADGRLTGLEVGRVGSLAKAFRDLIS
EGIAVADALAMTSSNAARILGLDTKGAIAEGKDADLVLLNDQFEPEFVWCLGKAMVANGLPLVRGTFEIV

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=389, Percent_Identity=40.6169665809769, Blast_Score=288, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 40003; Mature: 40003

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLIRNANLFTPSAVGLQDVLFGTGGVITIAEPGSLHVGSLPCHEVDAKGSALVPGLVD
CEEEEECCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCEECCHHHC
PLVHISGGGGEGGFHTRTPEMQLTEATLAGVTTLVGALGTDAVSRSLEDLLAKARGLANE
CCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
GLTTYIYTGSYEYPVKTLTGDITRDMMLIDKVIGVGEIAIADHRGSQLSAAELARVASQA
CCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
RVGGMLSGKGGIVFVHVGDGKGGLDILSEVAGTTDIPLSQFYPTHINRSRALLEQGVAFA
HHCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHCCCEEE
LAGGVIDFTASENSATLNSDEISAIDAVKHALAAGVAVSQITLSSDGNASLPVFDADGRL
EECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCE
TGLEVGRVGSLAKAFRDLISEGIAVADALAMTSSNAARILGLDTKGAIAEGKDADLVLLN
EEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEC
DQFEPEFVWCLGKAMVANGLPLVRGTFEIV
CCCCCHHHHHHHHHHHHCCCCEECCEEEEC
>Mature Secondary Structure
MLTLIRNANLFTPSAVGLQDVLFGTGGVITIAEPGSLHVGSLPCHEVDAKGSALVPGLVD
CEEEEECCCCCCCCCCCHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCCCEECCHHHC
PLVHISGGGGEGGFHTRTPEMQLTEATLAGVTTLVGALGTDAVSRSLEDLLAKARGLANE
CCEEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCC
GLTTYIYTGSYEYPVKTLTGDITRDMMLIDKVIGVGEIAIADHRGSQLSAAELARVASQA
CCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHH
RVGGMLSGKGGIVFVHVGDGKGGLDILSEVAGTTDIPLSQFYPTHINRSRALLEQGVAFA
HHCCEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHCCCEEE
LAGGVIDFTASENSATLNSDEISAIDAVKHALAAGVAVSQITLSSDGNASLPVFDADGRL
EECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEECCCCCE
TGLEVGRVGSLAKAFRDLISEGIAVADALAMTSSNAARILGLDTKGAIAEGKDADLVLLN
EEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEC
DQFEPEFVWCLGKAMVANGLPLVRGTFEIV
CCCCCHHHHHHHHHHHHCCCCEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]