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The map label for this gene is smpB

Identifier: 119775522

GI number: 119775522

Start: 2867670

End: 2868152

Strand: Direct

Name: smpB

Synonym: Sama_2388

Alternate gene names: 119775522

Gene position: 2867670-2868152 (Clockwise)

Preceding gene: 119775519

Following gene: 119775524

Centisome position: 66.59

GC content: 54.24

Gene sequence:

>483_bases
ATGGTAAAGAAAAACTCCAAAAAGGCCGCCCAGCCGGCCACGATCGCCCGCAATAAGCGCGCGACCTTCGAATACAAATT
CGATGAAAAATTCGAGGCAGGCCTATCCCTGATGGGATGGGAAGTGAAGTCTATCCGCGCCGGAAAAATCAACATCTCTG
AAAGCTACGTGATGCTGAAAAACGGTGAAGCCTTCCTGCACCACTGCCATATCACCCCACTGCACGCCGCCTCCACTCAC
GTGGTGTGTGACCCTACCCGCCCACGCAAGCTGCTGCTTAACCGCCGCGAGCTCGACCGCTTGGCAGGTCTCGTTGAGCG
CCAGGGCTATGCCATCGTGCCTATCTCCATGTACTGGCGCAAAGGTGCCTGGGTTAAGGTCGAGATTGGTCTGGGTAAAG
GTAAAAAAGCCCACGACAAACGCGATGACATCAAGCAGCGTGACTGGGACATTGAAAAAGCCCGGGTGATGAAGAACAAA
TAA

Upstream 100 bases:

>100_bases
ACTAAAAACTCACCAATAAAAACATTCGCTTGGCAAAACATGTAAATATTCGCAGGTGGTCAATTCCAAATTACACACCC
CACGCTATAATAGCCCGCCT

Downstream 100 bases:

>100_bases
ACTGTCTGTTTTCGAGCGGAAAACAGACAGTTGGTTGTCACTGCCAAATAATGCCTTGGAATTCGGGGCAGCATGGGAGT
ACAATCAAACCATCTTGGGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MVKKNSKKAAQPATIARNKRATFEYKFDEKFEAGLSLMGWEVKSIRAGKINISESYVMLKNGEAFLHHCHITPLHAASTH
VVCDPTRPRKLLLNRRELDRLAGLVERQGYAIVPISMYWRKGAWVKVEIGLGKGKKAHDKRDDIKQRDWDIEKARVMKNK

Sequences:

>Translated_160_residues
MVKKNSKKAAQPATIARNKRATFEYKFDEKFEAGLSLMGWEVKSIRAGKINISESYVMLKNGEAFLHHCHITPLHAASTH
VVCDPTRPRKLLLNRRELDRLAGLVERQGYAIVPISMYWRKGAWVKVEIGLGKGKKAHDKRDDIKQRDWDIEKARVMKNK
>Mature_160_residues
MVKKNSKKAAQPATIARNKRATFEYKFDEKFEAGLSLMGWEVKSIRAGKINISESYVMLKNGEAFLHHCHITPLHAASTH
VVCDPTRPRKLLLNRRELDRLAGLVERQGYAIVPISMYWRKGAWVKVEIGLGKGKKAHDKRDDIKQRDWDIEKARVMKNK

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=155, Percent_Identity=66.4516129032258, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_SHEAM (A1S886)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928262.1
- ProteinModelPortal:   A1S886
- SMR:   A1S886
- STRING:   A1S886
- GeneID:   4604637
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2388
- NMPDR:   fig|326297.7.peg.2236
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A1S886
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18411; Mature: 18411

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKNSKKAAQPATIARNKRATFEYKFDEKFEAGLSLMGWEVKSIRAGKINISESYVMLK
CCCCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCEEECEEECEEEECEEECCCCEEEEE
NGEAFLHHCHITPLHAASTHVVCDPTRPRKLLLNRRELDRLAGLVERQGYAIVPISMYWR
CCCEEEEEECCCEECCCCCEEEECCCCCHHHHHCHHHHHHHHHHHHCCCEEEEEEEHEEC
KGAWVKVEIGLGKGKKAHDKRDDIKQRDWDIEKARVMKNK
CCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHCCCC
>Mature Secondary Structure
MVKKNSKKAAQPATIARNKRATFEYKFDEKFEAGLSLMGWEVKSIRAGKINISESYVMLK
CCCCCCCCCCCCCHHCCCCCCEEEEECCCHHHCCCEEECEEECEEEECEEECCCCEEEEE
NGEAFLHHCHITPLHAASTHVVCDPTRPRKLLLNRRELDRLAGLVERQGYAIVPISMYWR
CCCEEEEEECCCEECCCCCEEEECCCCCHHHHHCHHHHHHHHHHHHCCCEEEEEEEHEEC
KGAWVKVEIGLGKGKKAHDKRDDIKQRDWDIEKARVMKNK
CCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA