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The map label for this gene is mlaA [H]

Identifier: 119775403

GI number: 119775403

Start: 2734603

End: 2735433

Strand: Reverse

Name: mlaA [H]

Synonym: Sama_2268

Alternate gene names: 119775403

Gene position: 2735433-2734603 (Counterclockwise)

Preceding gene: 119775406

Following gene: 119775402

Centisome position: 63.52

GC content: 48.62

Gene sequence:

>831_bases
ATGCTTTATTATCGTCAGCCACGGCGCTTTCTAAAGCCGATTTCGGCCTTTTGTATAGAGAGAGTTATGAAGTTGAAATG
GATTGCCTTGCCATTCATGTTGTTGGCAGGCTTGGCTCAGGCAGAGCAGGACAATTCGGTCTCAGAGAAGCAGGTAGACG
TTGTTTACAACGATCCCCGTGACCCTTTCGAAGGGTTTAACCGCGCCATGTGGGATCTTAACTACAACGTGCTTGACCGT
TATATCTATCGTCCCGTCGCCCACACCTACAAAGATTATGTGCCCAGTCCGGTAAAGTCGGGACTGCATAATTTTGTCCG
CAATCTTGAAGAGCCCAATGCCTTGGTGAACAACACACTTCAGGGGAAGTTTGGTTGGGCGGCCAACGCCGGCGGACGTT
TTGTGGTGAACTCCACCATAGGTCTGCTCGGGGTCGTTGATGTGGCCGACATGATGGGCATGCATCGGAAGCAGGATGAT
TTTAACGAGGTTTTGGGTTACTACGGTGTGCCCAATGGTCCTTACTTTATGGCGCCATTTTTTGGCCCTTATGTTACCCG
TGAACTCGCGAGTGATTGGGTCGATGGACTATACTTTCCCTTGTCAGAGCTGACGATGTGGCAGTCTGTGGTCAAATGGG
GCATCAAAAGCCTGCATGTGCGGACAGAAGCCATCGATCAGGAACGTCTGCTCGACAATGCTCTGGATCCCTACACCTTT
GTAAAAGATGCATATTTACAGCACATGGACTACAAGGTGTATGATGGCAATGTACCATCAAATCAAGAAGATGATGAACT
TCTTGATGAATATATGCAGGAGCTAGAATAG

Upstream 100 bases:

>100_bases
AAGTTGCCTGCATACACCAACTTACACAATTTATCGATTGATCTTATTGTGAGGAAAAGTATGCTTAGCCGTCAAAGTAG
CAGACGTGGTTCAGAGTGTT

Downstream 100 bases:

>100_bases
CCGTCCTCAAGGGATAACTCCGGCGGAGAGTCCCCCTCCTATGCGCTATCTGCTCCCTGAGGGAAGTATATGGCGCTAAA
AGATGTATCTGTGTTGTTGG

Product: vacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLYYRQPRRFLKPISAFCIERVMKLKWIALPFMLLAGLAQAEQDNSVSEKQVDVVYNDPRDPFEGFNRAMWDLNYNVLDR
YIYRPVAHTYKDYVPSPVKSGLHNFVRNLEEPNALVNNTLQGKFGWAANAGGRFVVNSTIGLLGVVDVADMMGMHRKQDD
FNEVLGYYGVPNGPYFMAPFFGPYVTRELASDWVDGLYFPLSELTMWQSVVKWGIKSLHVRTEAIDQERLLDNALDPYTF
VKDAYLQHMDYKVYDGNVPSNQEDDELLDEYMQELE

Sequences:

>Translated_276_residues
MLYYRQPRRFLKPISAFCIERVMKLKWIALPFMLLAGLAQAEQDNSVSEKQVDVVYNDPRDPFEGFNRAMWDLNYNVLDR
YIYRPVAHTYKDYVPSPVKSGLHNFVRNLEEPNALVNNTLQGKFGWAANAGGRFVVNSTIGLLGVVDVADMMGMHRKQDD
FNEVLGYYGVPNGPYFMAPFFGPYVTRELASDWVDGLYFPLSELTMWQSVVKWGIKSLHVRTEAIDQERLLDNALDPYTF
VKDAYLQHMDYKVYDGNVPSNQEDDELLDEYMQELE
>Mature_276_residues
MLYYRQPRRFLKPISAFCIERVMKLKWIALPFMLLAGLAQAEQDNSVSEKQVDVVYNDPRDPFEGFNRAMWDLNYNVLDR
YIYRPVAHTYKDYVPSPVKSGLHNFVRNLEEPNALVNNTLQGKFGWAANAGGRFVVNSTIGLLGVVDVADMMGMHRKQDD
FNEVLGYYGVPNGPYFMAPFFGPYVTRELASDWVDGLYFPLSELTMWQSVVKWGIKSLHVRTEAIDQERLLDNALDPYTF
VKDAYLQHMDYKVYDGNVPSNQEDDELLDEYMQELE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=258, Percent_Identity=36.4341085271318, Blast_Score=167, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 31973; Mature: 31973

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYYRQPRRFLKPISAFCIERVMKLKWIALPFMLLAGLAQAEQDNSVSEKQVDVVYNDPR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCC
DPFEGFNRAMWDLNYNVLDRYIYRPVAHTYKDYVPSPVKSGLHNFVRNLEEPNALVNNTL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCC
QGKFGWAANAGGRFVVNSTIGLLGVVDVADMMGMHRKQDDFNEVLGYYGVPNGPYFMAPF
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCH
FGPYVTRELASDWVDGLYFPLSELTMWQSVVKWGIKSLHVRTEAIDQERLLDNALDPYTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VKDAYLQHMDYKVYDGNVPSNQEDDELLDEYMQELE
HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLYYRQPRRFLKPISAFCIERVMKLKWIALPFMLLAGLAQAEQDNSVSEKQVDVVYNDPR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCC
DPFEGFNRAMWDLNYNVLDRYIYRPVAHTYKDYVPSPVKSGLHNFVRNLEEPNALVNNTL
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHCCC
QGKFGWAANAGGRFVVNSTIGLLGVVDVADMMGMHRKQDDFNEVLGYYGVPNGPYFMAPF
CCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEECCH
FGPYVTRELASDWVDGLYFPLSELTMWQSVVKWGIKSLHVRTEAIDQERLLDNALDPYTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VKDAYLQHMDYKVYDGNVPSNQEDDELLDEYMQELE
HHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]