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The map label for this gene is ogt [H]

Identifier: 119775339

GI number: 119775339

Start: 2661775

End: 2662314

Strand: Reverse

Name: ogt [H]

Synonym: Sama_2204

Alternate gene names: 119775339

Gene position: 2662314-2661775 (Counterclockwise)

Preceding gene: 119775340

Following gene: 119775338

Centisome position: 61.83

GC content: 57.59

Gene sequence:

>540_bases
ATGATGCGAATCGCCAATAATAGTTGCAAACTGCACGCACCCGCGCCGATGGCGGCGACTTGTACGCTGGAAAGTCCAGT
GGGCACCCTGTGGCTGGGGGCGAGCGAGAATGGCTTAATGACGCTCAAAGCCGCTCCCTTTAACGATGTGCCAGTGTATC
ACTCAGAGCAAGCCGTCACCCGGGCAAAAGGACATTTACAGGCGGCATGCTCGCAACTTGCCGAGTTTTTTGAAGGTAAG
CGCCATCAGTTTGATTTGGCGCTCGCCCCCAAAGGGACCGAGTTTCAGCGGGCCGTGTGGCAGGCGCTGCTCACCCTTCC
CTGGGGCGAAACGGCCAGTTATTCGGATATAGCCCTTCAAATCGCCAGGCCAAAGGCGGTGCGGGCCGTTGGGGCGGCCA
ATGGCGCCAATCCGGTGGCAATCATCATTCCTTGCCACCGAGTGATAGGTAAGGGCGGGGCTCTGACTGGCTATGCATGG
GGATTGAAAATGAAGGCGAGTCTGCTCAAACTCGAATCGGCAATACCTTCGCTGCGTTGA

Upstream 100 bases:

>100_bases
CTGACGATATATCAAGCCGATTTTACGCGAAGCAATAACCGGCCAAAGATAACCGTTTTGAGCGGCCCCCTGGAAACCCA
TTAATTCATAAAGAGGCACC

Downstream 100 bases:

>100_bases
TCCCGTCGATAAAAATACTGCGCTCAATCAAGTCCCGCCGAGCCCAGCCTCTGGCATAATGAGCTAAATAACCTCAGAGG
GGACTTGCATGAAACACCTG

Product: methylated-DNA--(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MMRIANNSCKLHAPAPMAATCTLESPVGTLWLGASENGLMTLKAAPFNDVPVYHSEQAVTRAKGHLQAACSQLAEFFEGK
RHQFDLALAPKGTEFQRAVWQALLTLPWGETASYSDIALQIARPKAVRAVGAANGANPVAIIIPCHRVIGKGGALTGYAW
GLKMKASLLKLESAIPSLR

Sequences:

>Translated_179_residues
MMRIANNSCKLHAPAPMAATCTLESPVGTLWLGASENGLMTLKAAPFNDVPVYHSEQAVTRAKGHLQAACSQLAEFFEGK
RHQFDLALAPKGTEFQRAVWQALLTLPWGETASYSDIALQIARPKAVRAVGAANGANPVAIIIPCHRVIGKGGALTGYAW
GLKMKASLLKLESAIPSLR
>Mature_179_residues
MMRIANNSCKLHAPAPMAATCTLESPVGTLWLGASENGLMTLKAAPFNDVPVYHSEQAVTRAKGHLQAACSQLAEFFEGK
RHQFDLALAPKGTEFQRAVWQALLTLPWGETASYSDIALQIARPKAVRAVGAANGANPVAIIIPCHRVIGKGGALTGYAW
GLKMKASLLKLESAIPSLR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787596, Length=165, Percent_Identity=38.7878787878788, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=56.8181818181818, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=52.4390243902439, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=52.4390243902439, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=93, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=99, Percent_Identity=43.4343434343434, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19074; Mature: 19074

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRIANNSCKLHAPAPMAATCTLESPVGTLWLGASENGLMTLKAAPFNDVPVYHSEQAVT
CCEECCCCCEEECCCCCEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCEECCHHHH
RAKGHLQAACSQLAEFFEGKRHQFDLALAPKGTEFQRAVWQALLTLPWGETASYSDIALQ
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
IARPKAVRAVGAANGANPVAIIIPCHRVIGKGGALTGYAWGLKMKASLLKLESAIPSLR
EECCHHHEEECCCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMRIANNSCKLHAPAPMAATCTLESPVGTLWLGASENGLMTLKAAPFNDVPVYHSEQAVT
CCEECCCCCEEECCCCCEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCEECCHHHH
RAKGHLQAACSQLAEFFEGKRHQFDLALAPKGTEFQRAVWQALLTLPWGETASYSDIALQ
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEE
IARPKAVRAVGAANGANPVAIIIPCHRVIGKGGALTGYAWGLKMKASLLKLESAIPSLR
EECCHHHEEECCCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA