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The map label for this gene is mdtE [H]

Identifier: 119775316

GI number: 119775316

Start: 2637764

End: 2638759

Strand: Direct

Name: mdtE [H]

Synonym: Sama_2181

Alternate gene names: 119775316

Gene position: 2637764-2638759 (Clockwise)

Preceding gene: 119775315

Following gene: 119775317

Centisome position: 61.26

GC content: 58.63

Gene sequence:

>996_bases
ATGACGACTTTCAAACAGCTCTTGGGACTGTCTCTGTTGGCCCTTGGCCTGAGTGCCCATGCTGGCCAAACCCTGATAGT
AGAACACACAGAAGAAGACAGATATTTAACCCTGGACGCCCTGATTGAGCCAGTTCAGGCCGCTACAGTCTCGGCGCAAA
CGTCGGGCCGGATCCTGAAAATCCATTTTGACGTCAACGACACTGTGCCTGCCGGTGCTGCCCTGCTGGAAATCACCAGC
AAGGAGCAGGGCGCTGCATTGGCAGCCGCAGAGGCCGACTACGCCCGCGCCCTGGCGGTGAACACTGAAGCACAGGCCAC
GTTGAGGCGCTATCAGGATCTTTTCCCCAAGGGCGCAATTTCCCGTGGCACCTTTGATGAGGCCATTGCCCGCGCCAAGA
GCAGCGAACAGGCCGTGACTGCCGCCAATGCCGCCATAGTGCGCGCCCGCGAATCCTTAAGCTACACCACTGTGTATGCC
CCATTTGGCGGCGTGCTCACCGAGCGCCATGTGGAACAGGGCGAAACCGTTAACCCAGGCCAGCCACTGCTGTCCGGCTT
CAGCGCCGATAAAATGCGGGCCGTGATGCAAGTGCCCGGTCGTTATCTCGCCGCCTTGAAGGCCGCCGGTGCCGTGAGTG
TGGAACTGGCGGATGGCCGCAGCATCGACAGCAATCAACTGACTATTTTCAGCTTTGCCTCCCCCCAAAGTCACAGCTTC
CAGGTACGTATCCAACTGCCAGACAATGCCGATGTGCAACCGGGCAGCTGGGCCAAGGCCAGTTTTGTACAGGGTAAACG
CCGCATGCTGCTGGTGCCCCAACGCGCCATTGTTTCCCGTGGTGAACTCACCGGTGTTTATATGATGCAGGGCGATAAAG
CCGTGTTGACCCAGGTGCGACTTGGCAAACAACAGGGTGACCGGGTACAGGTGCTCTCAGGCCTCAATGACGGTGACGTT
ATTGCCGCTGATGCCTATGCCGTAATTGCCCAGTGA

Upstream 100 bases:

>100_bases
TGTGTGTGAGTCACATTCCATAATTTTTTTCTAATGAAATGATTGCCGCCGTGGCAACTTTTCATTAGAATTTTCTCATA
GATTAAGCGGGAGCCTTTTT

Downstream 100 bases:

>100_bases
GGAATACAGGATGACGAGCGAAAACCTCCAACAGCTAGGGATTTCAGGCCGCATCGCCCGCGCGTTTCAGGCCAGTGCCA
TCACACCGCTGCTGGCGCTG

Product: AcrA/AcrE family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTTFKQLLGLSLLALGLSAHAGQTLIVEHTEEDRYLTLDALIEPVQAATVSAQTSGRILKIHFDVNDTVPAGAALLEITS
KEQGAALAAAEADYARALAVNTEAQATLRRYQDLFPKGAISRGTFDEAIARAKSSEQAVTAANAAIVRARESLSYTTVYA
PFGGVLTERHVEQGETVNPGQPLLSGFSADKMRAVMQVPGRYLAALKAAGAVSVELADGRSIDSNQLTIFSFASPQSHSF
QVRIQLPDNADVQPGSWAKASFVQGKRRMLLVPQRAIVSRGELTGVYMMQGDKAVLTQVRLGKQQGDRVQVLSGLNDGDV
IAADAYAVIAQ

Sequences:

>Translated_331_residues
MTTFKQLLGLSLLALGLSAHAGQTLIVEHTEEDRYLTLDALIEPVQAATVSAQTSGRILKIHFDVNDTVPAGAALLEITS
KEQGAALAAAEADYARALAVNTEAQATLRRYQDLFPKGAISRGTFDEAIARAKSSEQAVTAANAAIVRARESLSYTTVYA
PFGGVLTERHVEQGETVNPGQPLLSGFSADKMRAVMQVPGRYLAALKAAGAVSVELADGRSIDSNQLTIFSFASPQSHSF
QVRIQLPDNADVQPGSWAKASFVQGKRRMLLVPQRAIVSRGELTGVYMMQGDKAVLTQVRLGKQQGDRVQVLSGLNDGDV
IAADAYAVIAQ
>Mature_330_residues
TTFKQLLGLSLLALGLSAHAGQTLIVEHTEEDRYLTLDALIEPVQAATVSAQTSGRILKIHFDVNDTVPAGAALLEITSK
EQGAALAAAEADYARALAVNTEAQATLRRYQDLFPKGAISRGTFDEAIARAKSSEQAVTAANAAIVRARESLSYTTVYAP
FGGVLTERHVEQGETVNPGQPLLSGFSADKMRAVMQVPGRYLAALKAAGAVSVELADGRSIDSNQLTIFSFASPQSHSFQ
VRIQLPDNADVQPGSWAKASFVQGKRRMLLVPQRAIVSRGELTGVYMMQGDKAVLTQVRLGKQQGDRVQVLSGLNDGDVI
AADAYAVIAQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=298, Percent_Identity=27.5167785234899, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786668, Length=292, Percent_Identity=29.4520547945205, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789665, Length=292, Percent_Identity=27.0547945205479, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082036, Length=307, Percent_Identity=24.7557003257329, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 35236; Mature: 35105

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFKQLLGLSLLALGLSAHAGQTLIVEHTEEDRYLTLDALIEPVQAATVSAQTSGRILK
CCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHCCHHHHEEEECCCCCEEE
IHFDVNDTVPAGAALLEITSKEQGAALAAAEADYARALAVNTEAQATLRRYQDLFPKGAI
EEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHEEEEECCHHHHHHHHHHHHCCCCCC
SRGTFDEAIARAKSSEQAVTAANAAIVRARESLSYTTVYAPFGGVLTERHVEQGETVNPG
CCCCHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCC
QPLLSGFSADKMRAVMQVPGRYLAALKAAGAVSVELADGRSIDSNQLTIFSFASPQSHSF
CHHHCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCCCCE
QVRIQLPDNADVQPGSWAKASFVQGKRRMLLVPQRAIVSRGELTGVYMMQGDKAVLTQVR
EEEEECCCCCCCCCCCCCHHHHHCCCCEEEEECHHHHHCCCCEEEEEEEECCHHHHHHHH
LGKQQGDRVQVLSGLNDGDVIAADAYAVIAQ
HCCCCCCEEEEEECCCCCCEEEECCEEEEEC
>Mature Secondary Structure 
TTFKQLLGLSLLALGLSAHAGQTLIVEHTEEDRYLTLDALIEPVQAATVSAQTSGRILK
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEHHHHHCCHHHHEEEECCCCCEEE
IHFDVNDTVPAGAALLEITSKEQGAALAAAEADYARALAVNTEAQATLRRYQDLFPKGAI
EEECCCCCCCCCEEEEEEECCCCCCEEEECCCCHHEEEEECCHHHHHHHHHHHHCCCCCC
SRGTFDEAIARAKSSEQAVTAANAAIVRARESLSYTTVYAPFGGVLTERHVEQGETVNPG
CCCCHHHHHHHCCCCCHHHHHHHHHHEEHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCC
QPLLSGFSADKMRAVMQVPGRYLAALKAAGAVSVELADGRSIDSNQLTIFSFASPQSHSF
CHHHCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCCCCE
QVRIQLPDNADVQPGSWAKASFVQGKRRMLLVPQRAIVSRGELTGVYMMQGDKAVLTQVR
EEEEECCCCCCCCCCCCCHHHHHCCCCEEEEECHHHHHCCCCEEEEEEEECCHHHHHHHH
LGKQQGDRVQVLSGLNDGDVIAADAYAVIAQ
HCCCCCCEEEEEECCCCCCEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]