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The map label for this gene is mnmC

Identifier: 119775290

GI number: 119775290

Start: 2604195

End: 2606009

Strand: Direct

Name: mnmC

Synonym: Sama_2155

Alternate gene names: 119775290

Gene position: 2604195-2606009 (Clockwise)

Preceding gene: 119775279

Following gene: 119775296

Centisome position: 60.48

GC content: 55.43

Gene sequence:

>1815_bases
ATGCCAATTTCGCGACATCGAGTAAATTTTTTTAACCCCATGCCCGAGTTTTTTGCCATCGATATGGCCATGCCATTATC
ACTGCCCCTGCCTGAAGGCCCCTGTGTTGTGCTGTTTGAGGCGCTGCCTGAACCACCGCTTTGGCAGTGGCTGCTCGCTG
CGGCGCAGGTGAATTCTGCTTATCCAGAAACAAATGCTGCCTATGCCAGCAACGCTTCAATCACCAGCATACAGCATACG
AGCAAAAAGCCATTACAGATCATCATGCTGCTAAAAGATGAAGCTGAGCTTAACGCCCTGGCTATGGATGCCAGCCACCC
GCTCTCGCCTTTTGCCATGGCCGCGAGCCACATTAAAGGCGGACAAAGGTTTCATCTTGATGACAACTGCTGGCTGGACT
TTTACCTTTGTGAACCAAACAAAGCTGCCACACTCGCTACCTTTGATAAGGCCAGTATCGATGTGCTCATCTGCCCCAAG
GGCGAATTTGAGCAGGCAGCGCTTGAGCTTGCACACCAAGGCAGCATCCTGATTGGTGATACCAAGCCAGACATGCCAGC
AGCGCTTCCCATTAAGCCGCCCTTCAAACCAAGAGATATCAGCATTGTCGGCGCTGGCGTGGCCGGGGCAAGCCTTGCAC
TGTCGCTGCTTCGCCGTGGCCACAAGGTGACACTTTACTGCGCCGATGCCGCCCCCGGCATTGGTGCTTCCGGCAATCGG
CAGGGTGCCATCTATCCGCTGCTGACGCCAGAGGACGACCATCTGACCCGGCTGTTTGTACCGGCATTTTTGTATGCTAA
ACGCTTTGTCGAGTCGCTGGCACACCACCCCCTGCCCTTTGATTTTTGCGGCGTACTGCAAACCGGCCATGATGAACGCG
CCACTGAACGCATTGAAAAACTGTTGAGTAAAACCTGGCCCAAACAAGTGGCGGTCAGGGTTGATGCCGATGAGGCAAAT
CACATCGCCGGCGTCGCCATCGATAAAGGCGGTATTTATTACCCCCAAGGCGGCTGGGTTTCACCGGCCAAGCTGTGTGA
AGCAGCCATTGAAGAAGCCAAAACTATGGGCCTTGAGTGCCATTTCGATGTGAACATCACATCCATAACGCGCGCTGATG
AGGGCTGGCGACTGAACACTCAGACAGGCGAGATAGACGCCCATGAATTGGTGCTGGCTACCGGCCACAGGATCACTGAA
CTTGCACAAACCAAGTCGCTGCAGCTGGCGCCATTTCGCGGTCAGGTGAGCCATGTGCCCACCCGGGGTAAGCTTGAGCA
GCTTAAAACCGTTCTCTGTGCACACGGTTATTTCACTCCGCGCGACGCTAGCAGCGGTAGCCACTGCATGGGCGCAAGTT
ATGTGCGCGGTGATACTTCAGTTGAGTATCGCGAGCAGGAGCAGCAGGAAAACCTGCACAAGATGCAGGAAAGTTACCCA
GGCAAGGATTGGGTAGAGTATTTGGATATCAGTGAAGGCAGCGCCCGCGCCGGTATTCGAATGGTGAGCCGCGATCACCT
GCCGATGGCGGGCGCCGCGCCGGATGTGGCGGCAATTCAGACACAATATCAGGCCATTGGCCACGGCCCCAAGGGAGCCC
AGTACTGGCAAACCCACGCTGCGCCTGTGCATCCGGGGCTTTATATCTTCTCAGGCCTTGGCTCACGGGGATTATCCAGC
GGCCCGCTGATTGCCGAATGTCTGGCTGACAGCCTCTCAGGCACACTGCCTGTGCTGGATGGCGACGTGATGGAAGCCTT
AAACCCCAATCGAATGTGGCTTAGAAAGCTTAAAAAAGGCAAGGCACTGGACTGA

Upstream 100 bases:

>100_bases
TTACTATCCATTCCGTACTGGGAGTACCAATTTTAGTGCGCCAATAGTATCCCCTTTGGCGCTTTTAAGTGGTCAGCTTT
CCATAAAGGGCGGTAAAATA

Downstream 100 bases:

>100_bases
TAACAGCTTACCTACGAAAAGCAAAAAGGCAGGTTTAACCTGCCTTTTTTGTATTCAACACCTGGCGACTTCTACCGTTA
AAACTGGCCGCGCAGCTGAG

Product: glycine/D-amino acid oxidase (deaminating)-like protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MPISRHRVNFFNPMPEFFAIDMAMPLSLPLPEGPCVVLFEALPEPPLWQWLLAAAQVNSAYPETNAAYASNASITSIQHT
SKKPLQIIMLLKDEAELNALAMDASHPLSPFAMAASHIKGGQRFHLDDNCWLDFYLCEPNKAATLATFDKASIDVLICPK
GEFEQAALELAHQGSILIGDTKPDMPAALPIKPPFKPRDISIVGAGVAGASLALSLLRRGHKVTLYCADAAPGIGASGNR
QGAIYPLLTPEDDHLTRLFVPAFLYAKRFVESLAHHPLPFDFCGVLQTGHDERATERIEKLLSKTWPKQVAVRVDADEAN
HIAGVAIDKGGIYYPQGGWVSPAKLCEAAIEEAKTMGLECHFDVNITSITRADEGWRLNTQTGEIDAHELVLATGHRITE
LAQTKSLQLAPFRGQVSHVPTRGKLEQLKTVLCAHGYFTPRDASSGSHCMGASYVRGDTSVEYREQEQQENLHKMQESYP
GKDWVEYLDISEGSARAGIRMVSRDHLPMAGAAPDVAAIQTQYQAIGHGPKGAQYWQTHAAPVHPGLYIFSGLGSRGLSS
GPLIAECLADSLSGTLPVLDGDVMEALNPNRMWLRKLKKGKALD

Sequences:

>Translated_604_residues
MPISRHRVNFFNPMPEFFAIDMAMPLSLPLPEGPCVVLFEALPEPPLWQWLLAAAQVNSAYPETNAAYASNASITSIQHT
SKKPLQIIMLLKDEAELNALAMDASHPLSPFAMAASHIKGGQRFHLDDNCWLDFYLCEPNKAATLATFDKASIDVLICPK
GEFEQAALELAHQGSILIGDTKPDMPAALPIKPPFKPRDISIVGAGVAGASLALSLLRRGHKVTLYCADAAPGIGASGNR
QGAIYPLLTPEDDHLTRLFVPAFLYAKRFVESLAHHPLPFDFCGVLQTGHDERATERIEKLLSKTWPKQVAVRVDADEAN
HIAGVAIDKGGIYYPQGGWVSPAKLCEAAIEEAKTMGLECHFDVNITSITRADEGWRLNTQTGEIDAHELVLATGHRITE
LAQTKSLQLAPFRGQVSHVPTRGKLEQLKTVLCAHGYFTPRDASSGSHCMGASYVRGDTSVEYREQEQQENLHKMQESYP
GKDWVEYLDISEGSARAGIRMVSRDHLPMAGAAPDVAAIQTQYQAIGHGPKGAQYWQTHAAPVHPGLYIFSGLGSRGLSS
GPLIAECLADSLSGTLPVLDGDVMEALNPNRMWLRKLKKGKALD
>Mature_603_residues
PISRHRVNFFNPMPEFFAIDMAMPLSLPLPEGPCVVLFEALPEPPLWQWLLAAAQVNSAYPETNAAYASNASITSIQHTS
KKPLQIIMLLKDEAELNALAMDASHPLSPFAMAASHIKGGQRFHLDDNCWLDFYLCEPNKAATLATFDKASIDVLICPKG
EFEQAALELAHQGSILIGDTKPDMPAALPIKPPFKPRDISIVGAGVAGASLALSLLRRGHKVTLYCADAAPGIGASGNRQ
GAIYPLLTPEDDHLTRLFVPAFLYAKRFVESLAHHPLPFDFCGVLQTGHDERATERIEKLLSKTWPKQVAVRVDADEANH
IAGVAIDKGGIYYPQGGWVSPAKLCEAAIEEAKTMGLECHFDVNITSITRADEGWRLNTQTGEIDAHELVLATGHRITEL
AQTKSLQLAPFRGQVSHVPTRGKLEQLKTVLCAHGYFTPRDASSGSHCMGASYVRGDTSVEYREQEQQENLHKMQESYPG
KDWVEYLDISEGSARAGIRMVSRDHLPMAGAAPDVAAIQTQYQAIGHGPKGAQYWQTHAAPVHPGLYIFSGLGSRGLSSG
PLIAECLADSLSGTLPVLDGDVMEALNPNRMWLRKLKKGKALD

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=470, Percent_Identity=41.4893617021277, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_SHEAM (A1S7K4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928030.1
- STRING:   A1S7K4
- GeneID:   4604405
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2155
- eggNOG:   COG0665
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO

EC number: =2.1.1.61

Molecular weight: Translated: 65767; Mature: 65636

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISRHRVNFFNPMPEFFAIDMAMPLSLPLPEGPCVVLFEALPEPPLWQWLLAAAQVNSA
CCCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
YPETNAAYASNASITSIQHTSKKPLQIIMLLKDEAELNALAMDASHPLSPFAMAASHIKG
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCC
GQRFHLDDNCWLDFYLCEPNKAATLATFDKASIDVLICPKGEFEQAALELAHQGSILIGD
CCEEEECCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEEEEC
TKPDMPAALPIKPPFKPRDISIVGAGVAGASLALSLLRRGHKVTLYCADAAPGIGASGNR
CCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
QGAIYPLLTPEDDHLTRLFVPAFLYAKRFVESLAHHPLPFDFCGVLQTGHDERATERIEK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHH
LLSKTWPKQVAVRVDADEANHIAGVAIDKGGIYYPQGGWVSPAKLCEAAIEEAKTMGLEC
HHHHCCCCCEEEEEECCCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCEEE
HFDVNITSITRADEGWRLNTQTGEIDAHELVLATGHRITELAQTKSLQLAPFRGQVSHVP
EEECEEEEEEECCCCCEEECCCCCCCHHEEEEECCCHHHHHHHHCCEEECCCCCCCCCCC
TRGKLEQLKTVLCAHGYFTPRDASSGSHCMGASYVRGDTSVEYREQEQQENLHKMQESYP
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
GKDWVEYLDISEGSARAGIRMVSRDHLPMAGAAPDVAAIQTQYQAIGHGPKGAQYWQTHA
CCHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCC
APVHPGLYIFSGLGSRGLSSGPLIAECLADSLSGTLPVLDGDVMEALNPNRMWLRKLKKG
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHCCCCHHHHHHHHCC
KALD
CCCC
>Mature Secondary Structure 
PISRHRVNFFNPMPEFFAIDMAMPLSLPLPEGPCVVLFEALPEPPLWQWLLAAAQVNSA
CCCCCCCCCCCCCHHHEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCC
YPETNAAYASNASITSIQHTSKKPLQIIMLLKDEAELNALAMDASHPLSPFAMAASHIKG
CCCCCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCC
GQRFHLDDNCWLDFYLCEPNKAATLATFDKASIDVLICPKGEFEQAALELAHQGSILIGD
CCEEEECCCCEEEEEEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCCEEEEC
TKPDMPAALPIKPPFKPRDISIVGAGVAGASLALSLLRRGHKVTLYCADAAPGIGASGNR
CCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCC
QGAIYPLLTPEDDHLTRLFVPAFLYAKRFVESLAHHPLPFDFCGVLQTGHDERATERIEK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHH
LLSKTWPKQVAVRVDADEANHIAGVAIDKGGIYYPQGGWVSPAKLCEAAIEEAKTMGLEC
HHHHCCCCCEEEEEECCCCCCEEEEEEECCCEECCCCCCCCHHHHHHHHHHHHHHCCEEE
HFDVNITSITRADEGWRLNTQTGEIDAHELVLATGHRITELAQTKSLQLAPFRGQVSHVP
EEECEEEEEEECCCCCEEECCCCCCCHHEEEEECCCHHHHHHHHCCEEECCCCCCCCCCC
TRGKLEQLKTVLCAHGYFTPRDASSGSHCMGASYVRGDTSVEYREQEQQENLHKMQESYP
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCC
GKDWVEYLDISEGSARAGIRMVSRDHLPMAGAAPDVAAIQTQYQAIGHGPKGAQYWQTHA
CCHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCC
APVHPGLYIFSGLGSRGLSSGPLIAECLADSLSGTLPVLDGDVMEALNPNRMWLRKLKKG
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEECCHHHHHCCCCHHHHHHHHCC
KALD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA