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The map label for this gene is truA

Identifier: 119775285

GI number: 119775285

Start: 2596714

End: 2597499

Strand: Reverse

Name: truA

Synonym: Sama_2150

Alternate gene names: 119775285

Gene position: 2597499-2596714 (Counterclockwise)

Preceding gene: 119775286

Following gene: 119775284

Centisome position: 60.32

GC content: 55.6

Gene sequence:

>786_bases
ATGCGCATTGCTCTTGGGATTGAATACGACGGCAGCAAGTTTTATGGCTGGCAGCGTCAGGCCGAAGTGAACTCGGTACA
GGCAGAGCTCGAACGCGCGCTCTCCATCGTTGCTAACGAGCCCATTGAGGTCCAGTGTGCCGGTCGTACCGATGCCGGGG
TACACGCCACCGGCCAGGTGGTGCATTTCGATACCAATGCCGTAAGAAAAGAAGGCGCCTGGACACTGGGTGTGAACGTT
CACCTGCCCGATGACATCGCCGTTCGCTGGGCCATGCCGGTGAACGATGATTTCCATGCCCGTTTTTCTGCCACCGCCCG
CCGTTATCGGTACGTTATCTTCAATCATAATTTCCGTCCCGGCATTTTGCGCAAGGGCGTGAGCCATTATCACGGCGATA
TCGATGTCGAGCGCATGCATGATGCCGCACAGGCGCTAATAGGTGAGCGGGACTTCACCAGTTTTCGCGCCATTGGCTGC
CAGTCCAATACGCCGTTTCGCAATGTACATAAGGTCAGCGTAAGCCGCCATGGCATGTATATCGTGGTCGATATCCAGGC
CAACGCCTTTTTGCACCATATGGTGCGCAATATCGTAGGTTCCCTTCTGGAAATCGGCCTTGGCAACCAACCACTTGACT
GGATGGCAACCTTGCTTGAGGCCAAAGACAGAAGCCAGGCTGCCGCCACCGCCAAGCCTCACGGACTTTATTTGGTCGAT
GTGACTTACCCGGAGAGTTTTGGTTTGCCCAAGCTGTCCCTCGGCCCGCTTTTTATGCCAGACTGA

Upstream 100 bases:

>100_bases
TAAACGGCGCCCCGAGCGCCGTTTTTTTATGCCCTCGATAGGCAGCGAATGTGGTAATATCGCCGCGCTTTATCGATTTT
GACTGGCAGGAAATATAGAA

Downstream 100 bases:

>100_bases
GGCTGCGGCGACATTCACCTCGGATAAGCCGGCAGCCTACTTAAGCGAACGTCAGTAACAAGTCACAACCACAACAACAT
ATCAAATTCAGTGGAAGCAA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIALGIEYDGSKFYGWQRQAEVNSVQAELERALSIVANEPIEVQCAGRTDAGVHATGQVVHFDTNAVRKEGAWTLGVNV
HLPDDIAVRWAMPVNDDFHARFSATARRYRYVIFNHNFRPGILRKGVSHYHGDIDVERMHDAAQALIGERDFTSFRAIGC
QSNTPFRNVHKVSVSRHGMYIVVDIQANAFLHHMVRNIVGSLLEIGLGNQPLDWMATLLEAKDRSQAAATAKPHGLYLVD
VTYPESFGLPKLSLGPLFMPD

Sequences:

>Translated_261_residues
MRIALGIEYDGSKFYGWQRQAEVNSVQAELERALSIVANEPIEVQCAGRTDAGVHATGQVVHFDTNAVRKEGAWTLGVNV
HLPDDIAVRWAMPVNDDFHARFSATARRYRYVIFNHNFRPGILRKGVSHYHGDIDVERMHDAAQALIGERDFTSFRAIGC
QSNTPFRNVHKVSVSRHGMYIVVDIQANAFLHHMVRNIVGSLLEIGLGNQPLDWMATLLEAKDRSQAAATAKPHGLYLVD
VTYPESFGLPKLSLGPLFMPD
>Mature_261_residues
MRIALGIEYDGSKFYGWQRQAEVNSVQAELERALSIVANEPIEVQCAGRTDAGVHATGQVVHFDTNAVRKEGAWTLGVNV
HLPDDIAVRWAMPVNDDFHARFSATARRYRYVIFNHNFRPGILRKGVSHYHGDIDVERMHDAAQALIGERDFTSFRAIGC
QSNTPFRNVHKVSVSRHGMYIVVDIQANAFLHHMVRNIVGSLLEIGLGNQPLDWMATLLEAKDRSQAAATAKPHGLYLVD
VTYPESFGLPKLSLGPLFMPD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=284, Percent_Identity=30.2816901408451, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=70, Blast_Score=389, Evalue=1e-109,
Organism=Drosophila melanogaster, GI116007752, Length=305, Percent_Identity=27.2131147540984, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SHEAM (A1S7J9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928025.1
- ProteinModelPortal:   A1S7J9
- SMR:   A1S7J9
- STRING:   A1S7J9
- GeneID:   4604400
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2150
- NMPDR:   fig|326297.7.peg.2018
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A1S7J9
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29146; Mature: 29146

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGSKFYGWQRQAEVNSVQAELERALSIVANEPIEVQCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFDTNAVRKEGAWTLGVNVHLPDDIAVRWAMPVNDDFHARFSATARRYRYVIFNHNFRP
EEECCHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCEEEEEEEEECCCCC
GILRKGVSHYHGDIDVERMHDAAQALIGERDFTSFRAIGCQSNTPFRNVHKVSVSRHGMY
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCEEEEEEECCCEE
IVVDIQANAFLHHMVRNIVGSLLEIGLGNQPLDWMATLLEAKDRSQAAATAKPHGLYLVD
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEEE
VTYPESFGLPKLSLGPLFMPD
EECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIALGIEYDGSKFYGWQRQAEVNSVQAELERALSIVANEPIEVQCAGRTDAGVHATGQV
CEEEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCEE
VHFDTNAVRKEGAWTLGVNVHLPDDIAVRWAMPVNDDFHARFSATARRYRYVIFNHNFRP
EEECCHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCHHHHHHCCEEEEEEEEECCCCC
GILRKGVSHYHGDIDVERMHDAAQALIGERDFTSFRAIGCQSNTPFRNVHKVSVSRHGMY
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCEEEEEEECCCEE
IVVDIQANAFLHHMVRNIVGSLLEIGLGNQPLDWMATLLEAKDRSQAAATAKPHGLYLVD
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCEEEEE
VTYPESFGLPKLSLGPLFMPD
EECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA