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The map label for this gene is folC [H]

Identifier: 119775284

GI number: 119775284

Start: 2595338

End: 2596603

Strand: Reverse

Name: folC [H]

Synonym: Sama_2149

Alternate gene names: 119775284

Gene position: 2596603-2595338 (Counterclockwise)

Preceding gene: 119775285

Following gene: 119775283

Centisome position: 60.3

GC content: 55.29

Gene sequence:

>1266_bases
ATGACGCAGGAGCAAGCCATGTCCCCGGGTTTAGGTGCCACCCTTGGCGAGTGGTTGGATTATCTGCTTGCAATTCACCC
CACCGAGATTGATATGGGGTTGGCCCGGGTCACTGAAGTGGCACAGCGACTGAGGGTAAAATCGTTAGGTGATATCCTGG
TGGTCACCGTGGGCGGTACCAACGGCAAGGGCAGTACCTGTGCCATGCTGGAAAACATATTGCGCCGCGCGGGTTACCGT
GTCGGGGTATACAGTTCGCCCCACATCCTTCATTACAACGAACGCATTCGTATTGATGGTGTTGATGCCCTCGATGCGGA
CATTATTAACGCCTTCGAGCGCATTGAAGAAGCAAGGGGTGACATTTCGCTGACCTTCTTCGAGTTTGCGACGCTGACGG
GGCTTTTGCTGTTCAAACAGGCCGGATTGGATGTGGCTATTTTGGAAGTAGGCCTCGGCGGCCGCCTGGATGCGACCAAT
CTGGTGGACGCCGATATTGCGGTGGTGACCTCGGTGGATCTCGACCATCAGGCTTATTTGGGCAATACCCGTGAGTCGGT
AGGACGGGAAAAAGCCGGAATTTTCCGCGCGGGTCGACCTGCCATTGTCGGCGAGCCCGATATGCCTTCAACCGTGGCTG
AGGTGGCAAACCACTGTGGCGCTTTGCTATATCGTGTAGGGCAGGACTTCCGTTACCAGCGGGAACAGAGCTCATGGTCG
TATCAGGGCAAGCTGCGTAGCCTGACCAATATGCCACTGCCACAATTGCCACTGCCCAATGCCGCAACCGTAATTGCTAC
CTGCGAAGCCATTGGCGAGCGTCTGAATTTGTCTGATACGGTGGTGATAGAGGGGATAGTCACGGCGCGCCTTGCGGGTC
GACTCGAGGAACTCAGCCAATCGCCGCTGGTCATTGCCGATGTGGCTCACAACCCCCATGCGGCGCGTTTTTTAAAGTCT
GAGTTGGTTCGCTGGCCGGGTAAACGTGTGCTGGCACTTTGCGGTATGCTCAAAGACAAAGATTATCGTGAAGTATTGAA
TATTATGCATCCGGCGGTTGCTGACTGGTATCTGGTGACATTGGAAGGCGAGCGCGGCACCCGCGCATCTGAACTGGCCG
CTGCGCTACCTGCCGGGGCCAAGGCCCATACCTTCGACACTATTGAGCAGGCCCGAGAGGCGATACTCAAGGATGCAGGC
GAGGGGGATGTGGTAATTGTCTTCGGTTCTTTTTACACTGTATCCGGATTTAAATCATTAGCTTAA

Upstream 100 bases:

>100_bases
ACATTCACCTCGGATAAGCCGGCAGCCTACTTAAGCGAACGTCAGTAACAAGTCACAACCACAACAACATATCAAATTCA
GTGGAAGCAAGGATAAATCG

Downstream 100 bases:

>100_bases
AGGGAAGCCATTTTGTCCAGTCAGTTTCAAAACCGTTTGGTTGGCACCATAGTGATGGTCGCATTGGGAGTGATTTTTTT
GCCCGACTTGTTGGATGGCA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGTNGKGSTCAMLENILRRAGYR
VGVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARGDISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATN
LVDADIAVVTSVDLDHQAYLGNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWS
YQGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQSPLVIADVAHNPHAARFLKS
ELVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVTLEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAG
EGDVVIVFGSFYTVSGFKSLA

Sequences:

>Translated_421_residues
MTQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGTNGKGSTCAMLENILRRAGYR
VGVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARGDISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATN
LVDADIAVVTSVDLDHQAYLGNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWS
YQGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQSPLVIADVAHNPHAARFLKS
ELVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVTLEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAG
EGDVVIVFGSFYTVSGFKSLA
>Mature_420_residues
TQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGTNGKGSTCAMLENILRRAGYRV
GVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARGDISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATNL
VDADIAVVTSVDLDHQAYLGNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWSY
QGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQSPLVIADVAHNPHAARFLKSE
LVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVTLEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAGE
GDVVIVFGSFYTVSGFKSLA

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=178, Percent_Identity=37.6404494382022, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI66932990, Length=178, Percent_Identity=37.6404494382022, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788654, Length=409, Percent_Identity=51.8337408312958, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17553150, Length=164, Percent_Identity=37.8048780487805, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71984923, Length=164, Percent_Identity=37.8048780487805, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17553148, Length=164, Percent_Identity=37.8048780487805, Blast_Score=125, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323760, Length=330, Percent_Identity=28.4848484848485, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324815, Length=198, Percent_Identity=31.3131313131313, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641571, Length=203, Percent_Identity=35.4679802955665, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24581568, Length=188, Percent_Identity=36.7021276595745, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45637; Mature: 45506

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGT
CCCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NGKGSTCAMLENILRRAGYRVGVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARG
CCCCCHHHHHHHHHHHCCCEEEECCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCC
DISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATNLVDADIAVVTSVDLDHQAYL
CEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHC
GNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWS
CCCHHHHCHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
YQGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQ
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCC
SPLVIADVAHNPHAARFLKSELVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVT
CCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEE
LEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAGEGDVVIVFGSFYTVSGFKSL
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCHHHC
A
C
>Mature Secondary Structure 
TQEQAMSPGLGATLGEWLDYLLAIHPTEIDMGLARVTEVAQRLRVKSLGDILVVTVGGT
CCHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NGKGSTCAMLENILRRAGYRVGVYSSPHILHYNERIRIDGVDALDADIINAFERIEEARG
CCCCCHHHHHHHHHHHCCCEEEECCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCC
DISLTFFEFATLTGLLLFKQAGLDVAILEVGLGGRLDATNLVDADIAVVTSVDLDHQAYL
CEEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCHHHC
GNTRESVGREKAGIFRAGRPAIVGEPDMPSTVAEVANHCGALLYRVGQDFRYQREQSSWS
CCCHHHHCHHHHCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCC
YQGKLRSLTNMPLPQLPLPNAATVIATCEAIGERLNLSDTVVIEGIVTARLAGRLEELSQ
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCC
SPLVIADVAHNPHAARFLKSELVRWPGKRVLALCGMLKDKDYREVLNIMHPAVADWYLVT
CCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCEEEEE
LEGERGTRASELAAALPAGAKAHTFDTIEQAREAILKDAGEGDVVIVFGSFYTVSGFKSL
EECCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]