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The map label for this gene is trpA

Identifier: 119775269

GI number: 119775269

Start: 2579988

End: 2580788

Strand: Direct

Name: trpA

Synonym: Sama_2134

Alternate gene names: 119775269

Gene position: 2579988-2580788 (Clockwise)

Preceding gene: 119775268

Following gene: 119775270

Centisome position: 59.91

GC content: 59.43

Gene sequence:

>801_bases
ATGAACCGATATTCGAACGCCTTTGCCCGCTTAAAAGCCGGATCACGGGGCGCCTTCGTGCCTTTCGTCACCCTGGGTGA
CCCAGGCATCGACGAGAGTCTCGCGATTATTGATGCCCTGGTTGAAGGCGGCGCCGATTGTCTTGAGCTGGGCTTTCCCT
TCTCAGACCCACTCGCCGATGGCCCGGTGATCCAGGGCGCCAATATCCGCGCCTTGGCCGCCGGAACAACCCCGGATACC
TGTTTCAAGATGATTGAGCAAATCCGCGCCAAGTATCCCGAACTGCCCATTGGCCTCTTGCTGTACGCCAATTTGGTATT
TGCCAATGGCATAGAAGCCTTCTACCAACGCGCCAAGGCTGCCGGGGTCGATTCTGTGCTGATTGCCGATGTGCCGGCAG
AAGAGTCAGCGCCTTTCGTTGCCGCCGCCAGGGCCGAAGGCATAGCGCCGATTTTTATCGCGCCCCCAAATGCCAGCGCC
GACACCCTGCGACTGGTCGCGTCCCTTGGGGAAGGTTACACCTACCTCTTGTCCCGCGCCGGAGTGACAGGCGCCGACAA
CAAAGCCGGTATGCCACTGGACTCGGTACTATCGGCCCTGAAGGAATTCAACGCGCCTCCACCGCTGCTGGGATTTGGCA
TTGCCGAACCTTCACAGGTTCAGGAAGCGATTGCTGCCGGGGCAGCCGGTGCCATTTCCGGCTCCGCGGTAGTCAAGCGT
ATCGAAGCCTTAAAGGACGATATGCCCAAGCTCCTGACTGAACTCAAGTCCTTTGCCAGCGCCATGAAGGCAGCCACCTG
A

Upstream 100 bases:

>100_bases
AGCAAATCATTGTGGTCAATCTCTCTGGCCGCGGTGATAAAGACATTTTTACCGTATCTGACATTTTAGACGGCAAGGCC
AGCGCATAAGGAGTACTGCC

Downstream 100 bases:

>100_bases
GGGTCTGAATGACGCAGCTTTCCCTCAAAAAAAGGGAACGAAGACAATAGATACAGCGGGCATGCCCGCTGTTTTTTTGC
CAAAAAAAGGTGGTCGACGT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MNRYSNAFARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLADGPVIQGANIRALAAGTTPDT
CFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKAAGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASA
DTLRLVASLGEGYTYLLSRAGVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVKR
IEALKDDMPKLLTELKSFASAMKAAT

Sequences:

>Translated_266_residues
MNRYSNAFARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLADGPVIQGANIRALAAGTTPDT
CFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKAAGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASA
DTLRLVASLGEGYTYLLSRAGVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVKR
IEALKDDMPKLLTELKSFASAMKAAT
>Mature_266_residues
MNRYSNAFARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLADGPVIQGANIRALAAGTTPDT
CFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKAAGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASA
DTLRLVASLGEGYTYLLSRAGVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVKR
IEALKDDMPKLLTELKSFASAMKAAT

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=266, Percent_Identity=63.9097744360902, Blast_Score=326, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_SHEAM (A1S7I3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928009.1
- ProteinModelPortal:   A1S7I3
- SMR:   A1S7I3
- STRING:   A1S7I3
- GeneID:   4604384
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2134
- NMPDR:   fig|326297.7.peg.2004
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A1S7I3
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27426; Mature: 27426

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRYSNAFARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLAD
CCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCC
GPVIQGANIRALAAGTTPDTCFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKA
CCEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASADTLRLVASLGEGYTYLLSRA
CCCCCEEEECCCCCCCCCEEEHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVKR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHCHHHHHHHH
IEALKDDMPKLLTELKSFASAMKAAT
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRYSNAFARLKAGSRGAFVPFVTLGDPGIDESLAIIDALVEGGADCLELGFPFSDPLAD
CCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCC
GPVIQGANIRALAAGTTPDTCFKMIEQIRAKYPELPIGLLLYANLVFANGIEAFYQRAKA
CCEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGVDSVLIADVPAEESAPFVAAARAEGIAPIFIAPPNASADTLRLVASLGEGYTYLLSRA
CCCCCEEEECCCCCCCCCEEEHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHC
GVTGADNKAGMPLDSVLSALKEFNAPPPLLGFGIAEPSQVQEAIAAGAAGAISGSAVVKR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHCCCHHCHHHHHHHH
IEALKDDMPKLLTELKSFASAMKAAT
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA