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The map label for this gene is purN [H]

Identifier: 119775233

GI number: 119775233

Start: 2542420

End: 2543058

Strand: Direct

Name: purN [H]

Synonym: Sama_2098

Alternate gene names: 119775233

Gene position: 2542420-2543058 (Clockwise)

Preceding gene: 119775232

Following gene: 119775234

Centisome position: 59.04

GC content: 56.18

Gene sequence:

>639_bases
ATGAGTTGTCGCGTCGTTGTCCTTATCTCCGGCAGTGGCAGTAATCTGCAGGCCATTATCGACCAGTGTCAGGGCCGTAG
CGGCGTTGAACTGGTTGGCGTTATCAGCAATAAGCCTGATGCCTATGGTCTGGTCCGTGCCCACCATGCCGAGATAAACA
CCAGCTGCGTTATCGCCAAAAAAGGTGAAAAACGCGCTGACTATGACGCGCGCCTCACGGCGGCCATTGAAGCTTATCAG
CCAGATCTGATTGTGTTGGCAGGCTTTATGCGCATTCTCAGCGAAGGCTTTGTCAGCCGCTATCTGGGTAAAATGCTGAA
TATCCATCCGTCACTGCTGCCCAAGTACACCGGGCTGGATACCCACCAGCGCGCCATCGATGCCGGTGATACTGAGCATG
GTGCCAGTGTGCATTTTGTCACCCCCGAGCTGGATGCCGGTCCTGTTATCCTGCAGGCCAAGGTACCCATCTATGAGGGT
GACGACGCTCAAGCGCTTGCCGAACGGGTACATGAGCAGGAACATGCCATCTACCCTCTGGTCGTCAAATGGTATGCCGC
AGGCCGCTTGAAAATGGACGCCAATGGCGCCTATCTCGATGGCAGCCTGATTGGTCCAGGCGGCTACGCGCCGGACTGA

Upstream 100 bases:

>100_bases
ACGCGGCCCTTGAACTCCTTAAGGCCGAAGGTGAAAACGCCTGGCACATCGGCCATATCGCCGCGCGTAATGGCGATGAA
GAGCAGGTGGAGATCCTCTG

Downstream 100 bases:

>100_bases
GCAGACTGAAGCGAGCAGCACACTCTCTTGCTGCTTAATGAAAAAAGAGGCCCTGGTGGCCTCTTTTTTTATCCGGCAAT
CTATTCTCCCGCGACCTGAC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSCRVVVLISGSGSNLQAIIDQCQGRSGVELVGVISNKPDAYGLVRAHHAEINTSCVIAKKGEKRADYDARLTAAIEAYQ
PDLIVLAGFMRILSEGFVSRYLGKMLNIHPSLLPKYTGLDTHQRAIDAGDTEHGASVHFVTPELDAGPVILQAKVPIYEG
DDAQALAERVHEQEHAIYPLVVKWYAAGRLKMDANGAYLDGSLIGPGGYAPD

Sequences:

>Translated_212_residues
MSCRVVVLISGSGSNLQAIIDQCQGRSGVELVGVISNKPDAYGLVRAHHAEINTSCVIAKKGEKRADYDARLTAAIEAYQ
PDLIVLAGFMRILSEGFVSRYLGKMLNIHPSLLPKYTGLDTHQRAIDAGDTEHGASVHFVTPELDAGPVILQAKVPIYEG
DDAQALAERVHEQEHAIYPLVVKWYAAGRLKMDANGAYLDGSLIGPGGYAPD
>Mature_211_residues
SCRVVVLISGSGSNLQAIIDQCQGRSGVELVGVISNKPDAYGLVRAHHAEINTSCVIAKKGEKRADYDARLTAAIEAYQP
DLIVLAGFMRILSEGFVSRYLGKMLNIHPSLLPKYTGLDTHQRAIDAGDTEHGASVHFVTPELDAGPVILQAKVPIYEGD
DAQALAERVHEQEHAIYPLVVKWYAAGRLKMDANGAYLDGSLIGPGGYAPD

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=194, Percent_Identity=44.8453608247423, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869995, Length=194, Percent_Identity=44.8453608247423, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI209869993, Length=194, Percent_Identity=44.8453608247423, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1788846, Length=209, Percent_Identity=59.8086124401914, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787483, Length=150, Percent_Identity=34, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=35.0282485875706, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=35.5329949238579, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582400, Length=179, Percent_Identity=48.0446927374302, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22795; Mature: 22664

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCRVVVLISGSGSNLQAIIDQCQGRSGVELVGVISNKPDAYGLVRAHHAEINTSCVIAK
CCEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEE
KGEKRADYDARLTAAIEAYQPDLIVLAGFMRILSEGFVSRYLGKMLNIHPSLLPKYTGLD
CCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THQRAIDAGDTEHGASVHFVTPELDAGPVILQAKVPIYEGDDAQALAERVHEQEHAIYPL
HHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCHHHE
VVKWYAAGRLKMDANGAYLDGSLIGPGGYAPD
EEEEECCCEEEECCCCCEECCEEECCCCCCCC
>Mature Secondary Structure 
SCRVVVLISGSGSNLQAIIDQCQGRSGVELVGVISNKPDAYGLVRAHHAEINTSCVIAK
CEEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEEEEE
KGEKRADYDARLTAAIEAYQPDLIVLAGFMRILSEGFVSRYLGKMLNIHPSLLPKYTGLD
CCCCCCCCCHHEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCC
THQRAIDAGDTEHGASVHFVTPELDAGPVILQAKVPIYEGDDAQALAERVHEQEHAIYPL
HHHHHHCCCCCCCCCEEEEECCCCCCCCEEEEEECCEEECCCHHHHHHHHHHHHCCHHHE
VVKWYAAGRLKMDANGAYLDGSLIGPGGYAPD
EEEEECCCEEEECCCCCEECCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]