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The map label for this gene is rnt [H]

Identifier: 119775228

GI number: 119775228

Start: 2537303

End: 2538079

Strand: Direct

Name: rnt [H]

Synonym: Sama_2093

Alternate gene names: 119775228

Gene position: 2537303-2538079 (Clockwise)

Preceding gene: 119775217

Following gene: 119775230

Centisome position: 58.92

GC content: 54.57

Gene sequence:

>777_bases
ATGACAGCCAAGGCGCCTTTTAGCATAATAGCGCCCTTTGCAACGACCAGAGAATTCAGGATGTCTGACGCCTTCAACCC
CAACAAGCTCAAGTACCGGTTCCGTGGCTATTATCCTGTGGTAATCGATGTAGAAACCGCCGGCTTCAATGCCCAGACCG
ATGCCTTGCTGGAAATAGCCGTCACCCTGCTGACTATGGACGATAACGGCATGCTGGCGCTGGATAAAACACTGCATTTT
CATATTGAGCCCTTTGAAGGTGCCAATCTCGAACCTGCTGCGCTGGCCTTTAACGGCATAGACCCAAGTAATCCCCTGCG
TGGGGCCGTCAGTGAAAAAGAGGCGATGCTGGAAATATTCAAGGCGGTGCGCAAAGGCATGAAAGCCGCTGATTGCCACA
GGGCCGTGATAGTGGCCCACAATGCCGCCTTCGACCATGGCTTTGTGACCAAGGCGATTGACCGCAGTGGCGTAAAACGC
TCGCCATTCCATCCCTTTGCCACTTTTGATACTGCCTGTTTGTCTGGTCTGGCCCTGGGGCATACAGTGCTGGCGCAGGC
CTGTAAAATCGCCGGTATTCCCTTCGACAATAAAGAAGCCCACAGCGCCCTCTACGATACCGAGCGTACCGCCGAGCTCT
TCTGTTATATCGTCAACCGCTGGAAGTCCCTCGGTGGCTGGCCATTACTGGGCGCCGAGGCTCTGGATGACGCCGAGAAT
ACGGCTGCGGATAAGGAGATCGATGATGAGGATACCCTGGGTAGCACTGGCGAATAA

Upstream 100 bases:

>100_bases
AAGCAGTTACGCCACATAGGAATTCTCACAATTCAATGCTTACTCTGGAACTATCGACCAATATTCCCGGTTCTTTAGCG
CAGATTTTGCGCAATCAAAC

Downstream 100 bases:

>100_bases
GCGACTCTTCCCCTCCATGTGCTGATAGCGCCGCATAAATCAAAAGCGCCTAAGCGAATCACTGAAATAAAAAAGCCGCC
CGAGGGCGGCTTTTTATGCG

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTAKAPFSIIAPFATTREFRMSDAFNPNKLKYRFRGYYPVVIDVETAGFNAQTDALLEIAVTLLTMDDNGMLALDKTLHF
HIEPFEGANLEPAALAFNGIDPSNPLRGAVSEKEAMLEIFKAVRKGMKAADCHRAVIVAHNAAFDHGFVTKAIDRSGVKR
SPFHPFATFDTACLSGLALGHTVLAQACKIAGIPFDNKEAHSALYDTERTAELFCYIVNRWKSLGGWPLLGAEALDDAEN
TAADKEIDDEDTLGSTGE

Sequences:

>Translated_258_residues
MTAKAPFSIIAPFATTREFRMSDAFNPNKLKYRFRGYYPVVIDVETAGFNAQTDALLEIAVTLLTMDDNGMLALDKTLHF
HIEPFEGANLEPAALAFNGIDPSNPLRGAVSEKEAMLEIFKAVRKGMKAADCHRAVIVAHNAAFDHGFVTKAIDRSGVKR
SPFHPFATFDTACLSGLALGHTVLAQACKIAGIPFDNKEAHSALYDTERTAELFCYIVNRWKSLGGWPLLGAEALDDAEN
TAADKEIDDEDTLGSTGE
>Mature_257_residues
TAKAPFSIIAPFATTREFRMSDAFNPNKLKYRFRGYYPVVIDVETAGFNAQTDALLEIAVTLLTMDDNGMLALDKTLHFH
IEPFEGANLEPAALAFNGIDPSNPLRGAVSEKEAMLEIFKAVRKGMKAADCHRAVIVAHNAAFDHGFVTKAIDRSGVKRS
PFHPFATFDTACLSGLALGHTVLAQACKIAGIPFDNKEAHSALYDTERTAELFCYIVNRWKSLGGWPLLGAEALDDAENT
AADKEIDDEDTLGSTGE

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=213, Percent_Identity=69.4835680751174, Blast_Score=308, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 28100; Mature: 27969

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKAPFSIIAPFATTREFRMSDAFNPNKLKYRFRGYYPVVIDVETAGFNAQTDALLEIA
CCCCCCHHEECCCCCCHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHEEE
VTLLTMDDNGMLALDKTLHFHIEPFEGANLEPAALAFNGIDPSNPLRGAVSEKEAMLEIF
EEEEEECCCCEEEEECEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHH
KAVRKGMKAADCHRAVIVAHNAAFDHGFVTKAIDRSGVKRSPFHPFATFDTACLSGLALG
HHHHHCCCHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
HTVLAQACKIAGIPFDNKEAHSALYDTERTAELFCYIVNRWKSLGGWPLLGAEALDDAEN
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHH
TAADKEIDDEDTLGSTGE
HCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAKAPFSIIAPFATTREFRMSDAFNPNKLKYRFRGYYPVVIDVETAGFNAQTDALLEIA
CCCCCHHEECCCCCCHHHHHCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHEEE
VTLLTMDDNGMLALDKTLHFHIEPFEGANLEPAALAFNGIDPSNPLRGAVSEKEAMLEIF
EEEEEECCCCEEEEECEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCCHHHHHHHHHHH
KAVRKGMKAADCHRAVIVAHNAAFDHGFVTKAIDRSGVKRSPFHPFATFDTACLSGLALG
HHHHHCCCHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
HTVLAQACKIAGIPFDNKEAHSALYDTERTAELFCYIVNRWKSLGGWPLLGAEALDDAEN
HHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHH
TAADKEIDDEDTLGSTGE
HCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA