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The map label for this gene is puuB [H]

Identifier: 119775212

GI number: 119775212

Start: 2515300

End: 2516589

Strand: Reverse

Name: puuB [H]

Synonym: Sama_2077

Alternate gene names: 119775212

Gene position: 2516589-2515300 (Counterclockwise)

Preceding gene: 119775213

Following gene: 119775207

Centisome position: 58.44

GC content: 53.57

Gene sequence:

>1290_bases
ATGCAAGCTCGTTGCGGCTCTTATTACAATGCCACCATCAAACACGAAACTGACTATCCGCGCTTAACTGAAGCAATTCA
AGCAGATGTGGCGGTGATCGGCGGTGGATTTACCGGCGTAAACACAGCGCTTGAACTGGCCGAACAGGGTTATAAAGTGG
TGCTGCTGGAAGCCAATAAGATTGCCTGGGGTGCCACAGGCCGTAACGGCGGCCAGGTCACAGGGTCGCTGTCAGGCGAT
CAGGCCATGACCAAACAGCTCAGAAACCGTATCGGCGCAGAAGCCGAGGATTATGTTTGGGCGCTGCGCTGGCGTGGTCA
CGATATCATCAAGAACCGGGTGGCTAAGTACAATATCGACTGTGATTTGCGCCATGGCCATTTGCACACAGCCTATAAAC
CAGCCCACATGAATGAGCTTAAATCCATGTTGGACGAAGCCCAGCGAAAGGGTATGGGCGATCTGGTGTCATTGGTCGAA
AAAGACGAGATGCCTCAGTATCTCGAATCGCCTCTCTACCATGGTGGCTTGCTCAATAAAAAGAACATGCACCTGCATTC
GGTTAATCTGTGTATCGGTGAGGCGAGGGCGGCAGAAAGCCTGGGTGTGCGGATTTTCGAAGATACGCAAGTATTGGATA
TCGTTGAGGGCCCTCGTCCGGTGGTGAAAACGGCCTTTGGCAGCGTCACTGCAAACAAGGTGCTCATTGCCGGCAACGCC
TATCACAAGCTGGGGCGCCCGAAACTCAGAGGCATGCTATTTCCGGCATCCCTGGGCAACTGTGCAACTGTACGCTTATC
AGACGAAGTGGCCAGTGCAATCAATCCACACAATCTTGCCGTGTATGATTGCCGCTTTGTACTCGATTATTATCGGCTTA
CCGCGGATAACCGCCTTATGTTCGGTGGCGGCACCAACTACAGTGGCCGTGATCCCAAGAATGTGGCCGAAGAGCTGCGC
CCTGCGATTGAACGCACCTTCCCGCGACTTAAAGGTGTGGAGATAGAATTCGCCTGGGCCGGTATGGCGGGGATTGTGAT
TAACCGCATACCGCAGCTTGGCAAAGTCTCCGAGCACGTCTACTACTGCCAGGGTTATTCGGGCCATGGGGTGGCGACCA
GCCACATCATGTCAGAAATCATGGCCAAAGCCATTGATGGTGATCTGCGTGAGTTTGGCCTCTTTGCCGGTATGAAGCAC
TGGCGCATTCCACTGAATGAATGGTTTGGCAATCAGGCGTTGGCCATTGGTATGCTTTACTATCAGCTGCGTGAGAGCCT
CCGGGGCTGA

Upstream 100 bases:

>100_bases
ACAAATTTTACAATGGGTAAACAAATTGCTGTTCACCCATTGACCTTCTGTTGTATATATCGCTAACATCCGTAAAAAAT
AAAAAACAACAGGACTCTCC

Downstream 100 bases:

>100_bases
CAGATGAAATAAAAAGGCCGCATCCAATGCGGCCTTTTTAATGTGCGTCTGCATTGCCAATGCTCAGTGGCTTTGCCCCA
AATCGACCTTGTCCATTTCC

Product: oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQARCGSYYNATIKHETDYPRLTEAIQADVAVIGGGFTGVNTALELAEQGYKVVLLEANKIAWGATGRNGGQVTGSLSGD
QAMTKQLRNRIGAEAEDYVWALRWRGHDIIKNRVAKYNIDCDLRHGHLHTAYKPAHMNELKSMLDEAQRKGMGDLVSLVE
KDEMPQYLESPLYHGGLLNKKNMHLHSVNLCIGEARAAESLGVRIFEDTQVLDIVEGPRPVVKTAFGSVTANKVLIAGNA
YHKLGRPKLRGMLFPASLGNCATVRLSDEVASAINPHNLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAEELR
PAIERTFPRLKGVEIEFAWAGMAGIVINRIPQLGKVSEHVYYCQGYSGHGVATSHIMSEIMAKAIDGDLREFGLFAGMKH
WRIPLNEWFGNQALAIGMLYYQLRESLRG

Sequences:

>Translated_429_residues
MQARCGSYYNATIKHETDYPRLTEAIQADVAVIGGGFTGVNTALELAEQGYKVVLLEANKIAWGATGRNGGQVTGSLSGD
QAMTKQLRNRIGAEAEDYVWALRWRGHDIIKNRVAKYNIDCDLRHGHLHTAYKPAHMNELKSMLDEAQRKGMGDLVSLVE
KDEMPQYLESPLYHGGLLNKKNMHLHSVNLCIGEARAAESLGVRIFEDTQVLDIVEGPRPVVKTAFGSVTANKVLIAGNA
YHKLGRPKLRGMLFPASLGNCATVRLSDEVASAINPHNLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAEELR
PAIERTFPRLKGVEIEFAWAGMAGIVINRIPQLGKVSEHVYYCQGYSGHGVATSHIMSEIMAKAIDGDLREFGLFAGMKH
WRIPLNEWFGNQALAIGMLYYQLRESLRG
>Mature_429_residues
MQARCGSYYNATIKHETDYPRLTEAIQADVAVIGGGFTGVNTALELAEQGYKVVLLEANKIAWGATGRNGGQVTGSLSGD
QAMTKQLRNRIGAEAEDYVWALRWRGHDIIKNRVAKYNIDCDLRHGHLHTAYKPAHMNELKSMLDEAQRKGMGDLVSLVE
KDEMPQYLESPLYHGGLLNKKNMHLHSVNLCIGEARAAESLGVRIFEDTQVLDIVEGPRPVVKTAFGSVTANKVLIAGNA
YHKLGRPKLRGMLFPASLGNCATVRLSDEVASAINPHNLAVYDCRFVLDYYRLTADNRLMFGGGTNYSGRDPKNVAEELR
PAIERTFPRLKGVEIEFAWAGMAGIVINRIPQLGKVSEHVYYCQGYSGHGVATSHIMSEIMAKAIDGDLREFGLFAGMKH
WRIPLNEWFGNQALAIGMLYYQLRESLRG

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=430, Percent_Identity=38.8372093023256, Blast_Score=308, Evalue=4e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47554; Mature: 47554

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQARCGSYYNATIKHETDYPRLTEAIQADVAVIGGGFTGVNTALELAEQGYKVVLLEANK
CCCCCCCCEEEEEEECCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCEEEEEECCE
IAWGATGRNGGQVTGSLSGDQAMTKQLRNRIGAEAEDYVWALRWRGHDIIKNRVAKYNID
EEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCHHEECCC
CDLRHGHLHTAYKPAHMNELKSMLDEAQRKGMGDLVSLVEKDEMPQYLESPLYHGGLLNK
EEEECCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
KNMHLHSVNLCIGEARAAESLGVRIFEDTQVLDIVEGPRPVVKTAFGSVTANKVLIAGNA
CCCEEEEEEEEECCHHHHHHCCEEEECCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCH
YHKLGRPKLRGMLFPASLGNCATVRLSDEVASAINPHNLAVYDCRFVLDYYRLTADNRLM
HHHCCCCHHCCEEECCCCCCEEEEEECHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEE
FGGGTNYSGRDPKNVAEELRPAIERTFPRLKGVEIEFAWAGMAGIVINRIPQLGKVSEHV
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCE
YYCQGYSGHGVATSHIMSEIMAKAIDGDLREFGLFAGMKHWRIPLNEWFGNQALAIGMLY
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCHHHHCCCCHHHHHHHH
YQLRESLRG
HHHHHHHCC
>Mature Secondary Structure
MQARCGSYYNATIKHETDYPRLTEAIQADVAVIGGGFTGVNTALELAEQGYKVVLLEANK
CCCCCCCCEEEEEEECCCCHHHHHHHHHCEEEEECCCHHHHHHHHHHHCCCEEEEEECCE
IAWGATGRNGGQVTGSLSGDQAMTKQLRNRIGAEAEDYVWALRWRGHDIIKNRVAKYNID
EEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCHHEECCC
CDLRHGHLHTAYKPAHMNELKSMLDEAQRKGMGDLVSLVEKDEMPQYLESPLYHGGLLNK
EEEECCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
KNMHLHSVNLCIGEARAAESLGVRIFEDTQVLDIVEGPRPVVKTAFGSVTANKVLIAGNA
CCCEEEEEEEEECCHHHHHHCCEEEECCCEEEEECCCCCHHHHHHHCCCCCCEEEEECCH
YHKLGRPKLRGMLFPASLGNCATVRLSDEVASAINPHNLAVYDCRFVLDYYRLTADNRLM
HHHCCCCHHCCEEECCCCCCEEEEEECHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEE
FGGGTNYSGRDPKNVAEELRPAIERTFPRLKGVEIEFAWAGMAGIVINRIPQLGKVSEHV
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCCCCCCE
YYCQGYSGHGVATSHIMSEIMAKAIDGDLREFGLFAGMKHWRIPLNEWFGNQALAIGMLY
EEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCHHCCCCHHHHCCCCHHHHHHHH
YQLRESLRG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]