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The map label for this gene is rluE [H]

Identifier: 119775195

GI number: 119775195

Start: 2496625

End: 2497263

Strand: Direct

Name: rluE [H]

Synonym: Sama_2060

Alternate gene names: 119775195

Gene position: 2496625-2497263 (Clockwise)

Preceding gene: 119775193

Following gene: 119775196

Centisome position: 57.98

GC content: 57.9

Gene sequence:

>639_bases
ATGTCAGAGAAAATCGCAGAACGGCCAAGCGCCAAGGCTGCGCCGCGCAAGCCCGCGGTTACCCAGCTGGTGCTGTTCAA
CAAGCCCTTCGATGTGTTGTGCCAGTTCACCGATGAAGCCGGCAGGCAAACCCTGAAGGACTTTATTCCTTTGCCCGGCG
TCTACGCCGCAGGCCGGCTCGACAGAGACAGTGAAGGCCTGTTGCTGCTCACCAACGATGGCCAGCTGCAATCCCGTCTT
ACCGAGCCGAAAAAGCAGACCTATAAAACCTATTGGGTGCAGGTCGAAGGCATTCCCGACGAATCGGCGCTGGATAAACT
GCGTGAGGGTGTTGAGCTTAAAGATGGCCCTACTCTGCCTGCCAGGGTACGCCGCATCGATGCGCCTCAAATTTGGCCGC
GCAATCCGCCGGTGCGGGAGCGAAAAAGCATTCCGGACTGTTGGCTCGAAATTCAGATTTGCGAAGGTCGCAACCGCCAG
GTTCGGCGCATGACGGCCCACATTGGCTATCCCACGTTAAGGCTGGTGCGCGCCGCCATAGGTGAGATAAGACTTGGACA
TCTTGGCAGCGGCGAGTACCGGAAGCTTAACCGGGACGAGATAAGTAGCCTGAGCACCACCCTTGGCATAAAGCGCTAG

Upstream 100 bases:

>100_bases
ACAGGGCGAGAAAAAAGGCTTTGCAACCAAAATTAGCCAGCAGACAGATGCAAAAAACCGTAAATCATTCAATGACAAAG
GACCGGGAACTGACAAACGG

Downstream 100 bases:

>100_bases
CTTAATAATTAAGGAAGCCCGATGGAGCGCTACAAACCCAATGTGACCGTTGCCTGTATTGTTCATTGTCAGGGCAAATT
CCTGATGGTGGAAGAAATCA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSEKIAERPSAKAAPRKPAVTQLVLFNKPFDVLCQFTDEAGRQTLKDFIPLPGVYAAGRLDRDSEGLLLLTNDGQLQSRL
TEPKKQTYKTYWVQVEGIPDESALDKLREGVELKDGPTLPARVRRIDAPQIWPRNPPVRERKSIPDCWLEIQICEGRNRQ
VRRMTAHIGYPTLRLVRAAIGEIRLGHLGSGEYRKLNRDEISSLSTTLGIKR

Sequences:

>Translated_212_residues
MSEKIAERPSAKAAPRKPAVTQLVLFNKPFDVLCQFTDEAGRQTLKDFIPLPGVYAAGRLDRDSEGLLLLTNDGQLQSRL
TEPKKQTYKTYWVQVEGIPDESALDKLREGVELKDGPTLPARVRRIDAPQIWPRNPPVRERKSIPDCWLEIQICEGRNRQ
VRRMTAHIGYPTLRLVRAAIGEIRLGHLGSGEYRKLNRDEISSLSTTLGIKR
>Mature_211_residues
SEKIAERPSAKAAPRKPAVTQLVLFNKPFDVLCQFTDEAGRQTLKDFIPLPGVYAAGRLDRDSEGLLLLTNDGQLQSRLT
EPKKQTYKTYWVQVEGIPDESALDKLREGVELKDGPTLPARVRRIDAPQIWPRNPPVRERKSIPDCWLEIQICEGRNRQV
RRMTAHIGYPTLRLVRAAIGEIRLGHLGSGEYRKLNRDEISSLSTTLGIKR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=198, Percent_Identity=62.1212121212121, Blast_Score=254, Evalue=2e-69,
Organism=Escherichia coli, GI1787524, Length=167, Percent_Identity=34.1317365269461, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788510, Length=186, Percent_Identity=34.4086021505376, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790453, Length=191, Percent_Identity=29.3193717277487, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 24022; Mature: 23891

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKIAERPSAKAAPRKPAVTQLVLFNKPFDVLCQFTDEAGRQTLKDFIPLPGVYAAGRL
CCCCHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DRDSEGLLLLTNDGQLQSRLTEPKKQTYKTYWVQVEGIPDESALDKLREGVELKDGPTLP
CCCCCCEEEEECCCHHHHHHCCHHHHCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCH
ARVRRIDAPQIWPRNPPVRERKSIPDCWLEIQICEGRNRQVRRMTAHIGYPTLRLVRAAI
HHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
GEIRLGHLGSGEYRKLNRDEISSLSTTLGIKR
HCEEECCCCCCCHHHCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEKIAERPSAKAAPRKPAVTQLVLFNKPFDVLCQFTDEAGRQTLKDFIPLPGVYAAGRL
CCCHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DRDSEGLLLLTNDGQLQSRLTEPKKQTYKTYWVQVEGIPDESALDKLREGVELKDGPTLP
CCCCCCEEEEECCCHHHHHHCCHHHHCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCH
ARVRRIDAPQIWPRNPPVRERKSIPDCWLEIQICEGRNRQVRRMTAHIGYPTLRLVRAAI
HHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHH
GEIRLGHLGSGEYRKLNRDEISSLSTTLGIKR
HCEEECCCCCCCHHHCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]