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The map label for this gene is cspD [H]

Identifier: 119775193

GI number: 119775193

Start: 2493616

End: 2493822

Strand: Direct

Name: cspD [H]

Synonym: Sama_2058

Alternate gene names: 119775193

Gene position: 2493616-2493822 (Clockwise)

Preceding gene: 119775190

Following gene: 119775195

Centisome position: 57.91

GC content: 46.86

Gene sequence:

>207_bases
ATGGCAAGCGGGACTGTTAAATGGTTCAACAACGCCAAAGGATTCGGGTTTATTTGCCCTGATGAAGGTGGTGAAGATGT
ATTTGCCCATTATTCAACTATCGAAATGGAAGGCTATCGAACTCTAAAAGCAGGCCAACCAGTCCAGTTCGAAGTTGAGC
AAGGTCCAAAGGGGATGCACGCTTCAGCAATCACACCTGCTCGATGA

Upstream 100 bases:

>100_bases
CTTTTAACGCTTTCATTCTGTTCAACGAAGCGGAGTTAGGAACCCGCTTAATTGGTTTTACTATCTTACTGGGAAGTAGA
CAACAGAAGGAAGTGGAAGT

Downstream 100 bases:

>100_bases
TGCGTCGAAAGGTAAAGTTAAAAAGGCAGGAATAATTTCCTGCCTTTTTATTTTTAGTTCTTTTCCCTGCCATTTATTTT
TAACACCCATTAAAAAAGGA

Product: cold-shock DNA-binding domain-containing protein

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 68; Mature: 67

Protein sequence:

>68_residues
MASGTVKWFNNAKGFGFICPDEGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAITPAR

Sequences:

>Translated_68_residues
MASGTVKWFNNAKGFGFICPDEGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAITPAR
>Mature_67_residues
ASGTVKWFNNAKGFGFICPDEGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMHASAITPAR

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=68, Percent_Identity=77.9411764705882, Blast_Score=118, Evalue=7e-29,
Organism=Escherichia coli, GI1789979, Length=63, Percent_Identity=53.968253968254, Blast_Score=79, Evalue=8e-17,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=52.4590163934426, Blast_Score=77, Evalue=3e-16,
Organism=Escherichia coli, GI1788126, Length=62, Percent_Identity=53.2258064516129, Blast_Score=75, Evalue=1e-15,
Organism=Escherichia coli, GI2367114, Length=63, Percent_Identity=49.2063492063492, Blast_Score=74, Evalue=1e-15,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=53.2258064516129, Blast_Score=74, Evalue=2e-15,
Organism=Escherichia coli, GI1787834, Length=65, Percent_Identity=49.2307692307692, Blast_Score=71, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24658520, Length=62, Percent_Identity=43.5483870967742, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7394; Mature: 7263

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGTVKWFNNAKGFGFICPDEGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMH
CCCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASAITPAR
CCCCCCCC
>Mature Secondary Structure 
ASGTVKWFNNAKGFGFICPDEGGEDVFAHYSTIEMEGYRTLKAGQPVQFEVEQGPKGMH
CCCEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCEEEECCCCCEEEEECCCCCCCC
ASAITPAR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]