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The map label for this gene is holB [H]

Identifier: 119775177

GI number: 119775177

Start: 2478933

End: 2479847

Strand: Reverse

Name: holB [H]

Synonym: Sama_2042

Alternate gene names: 119775177

Gene position: 2479847-2478933 (Counterclockwise)

Preceding gene: 119775178

Following gene: 119775176

Centisome position: 57.59

GC content: 50.93

Gene sequence:

>915_bases
ATGACTGCCATTCCCTGGCTTTCCCAGCCTTTGGCCGATTTTACGGCGCTGCTAACCGCCGGGCGCATGCCCCATGCGGT
GTTGGTGGGTGTGGATGAAGGGCTGGGCGCATCGATGCTGGTTAATCAACTTGCCCAAACAGCACTCTGCACCTCTCCCA
ATGTGTCTGGACCCTGTGGCCAATGTAAAAGCTGTCAGTTATTCAGTGCGGGAAATCATCCGGATTTTTACCCCATCAAA
GCCGATGGGACTCAAATTAAAATCGATCAAATCCGTGAACTCATTGCCCGTCTTTCGAGCACCGCCCAGCAAGGTGGCAG
GCGGGTAGCCATCATTGAAGCGGCTGAGCGCATGAATACCGCGTCTGCCAATGCGCTGTTGAAAACCCTGGAAGAGCCAG
GTGCCAACACCCTGATTTTATTGCACAGTAACACGCCTGCACGCTTGCTTCCCACCTTGCAGAGTCGCTGTCAAAAGTTG
GCGTTTGTGGCACCTAACCAACGCCAGATCGCGGCCTGGCTGGCTCAGCAGGGGCTTGGCACAGACGCTCTCTGGTGTTT
GCCTGTGGTGGGTGGCCCCATCAAATTGGCAGAGTACTTGCAAAACAATTACATAAGCGTACTTGAAAACATCAGAAAAG
ATTGGCGAAGCAGTTTGTCGACGGGCCATCTGTGTGCTAGCTTGAAAGAAATTGCCGAAGACCAAATAACTGATGCTCTT
AAGGTTTTGTACGTTGAATTGATGGCCTACCTCGCTGCAGCAGCAAAAGAGGATCCGCTTTTGGCATCACGGCTGGCAGG
ATTGGCTGGTGAAGTTATGAGCGTTTGTCATACGTTAAGTACTATGCCGAGCGTCAATTATCTGGCACTATGTCAAAAGA
TTGTTTCAGAATATCAGAGAATAAAAGAAAACTAG

Upstream 100 bases:

>100_bases
CAGCTCCATAGTGGTAATTGACGCTTCCAAGCCGCTTAACGAGGTACACAAGGACATTCTTGCCTGCTTAAGCACAGCCC
TGTAGAGGGCAAGGAGACCT

Downstream 100 bases:

>100_bases
CCTATGGTAGATCTGGTGGTTAACTTCGATACCCTGCATCAGCTGTATCGTGCCTATATGCCTTTTATCAAGCCGGCGGG
GCTCTTTGTTTCCACCAGCG

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTAIPWLSQPLADFTALLTAGRMPHAVLVGVDEGLGASMLVNQLAQTALCTSPNVSGPCGQCKSCQLFSAGNHPDFYPIK
ADGTQIKIDQIRELIARLSSTAQQGGRRVAIIEAAERMNTASANALLKTLEEPGANTLILLHSNTPARLLPTLQSRCQKL
AFVAPNQRQIAAWLAQQGLGTDALWCLPVVGGPIKLAEYLQNNYISVLENIRKDWRSSLSTGHLCASLKEIAEDQITDAL
KVLYVELMAYLAAAAKEDPLLASRLAGLAGEVMSVCHTLSTMPSVNYLALCQKIVSEYQRIKEN

Sequences:

>Translated_304_residues
MTAIPWLSQPLADFTALLTAGRMPHAVLVGVDEGLGASMLVNQLAQTALCTSPNVSGPCGQCKSCQLFSAGNHPDFYPIK
ADGTQIKIDQIRELIARLSSTAQQGGRRVAIIEAAERMNTASANALLKTLEEPGANTLILLHSNTPARLLPTLQSRCQKL
AFVAPNQRQIAAWLAQQGLGTDALWCLPVVGGPIKLAEYLQNNYISVLENIRKDWRSSLSTGHLCASLKEIAEDQITDAL
KVLYVELMAYLAAAAKEDPLLASRLAGLAGEVMSVCHTLSTMPSVNYLALCQKIVSEYQRIKEN
>Mature_303_residues
TAIPWLSQPLADFTALLTAGRMPHAVLVGVDEGLGASMLVNQLAQTALCTSPNVSGPCGQCKSCQLFSAGNHPDFYPIKA
DGTQIKIDQIRELIARLSSTAQQGGRRVAIIEAAERMNTASANALLKTLEEPGANTLILLHSNTPARLLPTLQSRCQKLA
FVAPNQRQIAAWLAQQGLGTDALWCLPVVGGPIKLAEYLQNNYISVLENIRKDWRSSLSTGHLCASLKEIAEDQITDALK
VLYVELMAYLAAAAKEDPLLASRLAGLAGEVMSVCHTLSTMPSVNYLALCQKIVSEYQRIKEN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=40.3314917127072, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786676, Length=175, Percent_Identity=31.4285714285714, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 32779; Mature: 32648

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIPWLSQPLADFTALLTAGRMPHAVLVGVDEGLGASMLVNQLAQTALCTSPNVSGPCG
CCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
QCKSCQLFSAGNHPDFYPIKADGTQIKIDQIRELIARLSSTAQQGGRRVAIIEAAERMNT
CCCCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCH
ASANALLKTLEEPGANTLILLHSNTPARLLPTLQSRCQKLAFVAPNQRQIAAWLAQQGLG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
TDALWCLPVVGGPIKLAEYLQNNYISVLENIRKDWRSSLSTGHLCASLKEIAEDQITDAL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KVLYVELMAYLAAAAKEDPLLASRLAGLAGEVMSVCHTLSTMPSVNYLALCQKIVSEYQR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IKEN
HHCC
>Mature Secondary Structure 
TAIPWLSQPLADFTALLTAGRMPHAVLVGVDEGLGASMLVNQLAQTALCTSPNVSGPCG
CCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
QCKSCQLFSAGNHPDFYPIKADGTQIKIDQIRELIARLSSTAQQGGRRVAIIEAAERMNT
CCCCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHCH
ASANALLKTLEEPGANTLILLHSNTPARLLPTLQSRCQKLAFVAPNQRQIAAWLAQQGLG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
TDALWCLPVVGGPIKLAEYLQNNYISVLENIRKDWRSSLSTGHLCASLKEIAEDQITDAL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
KVLYVELMAYLAAAAKEDPLLASRLAGLAGEVMSVCHTLSTMPSVNYLALCQKIVSEYQR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
IKEN
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]