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The map label for this gene is ilvI [H]

Identifier: 119775153

GI number: 119775153

Start: 2453387

End: 2455108

Strand: Direct

Name: ilvI [H]

Synonym: Sama_2018

Alternate gene names: 119775153

Gene position: 2453387-2455108 (Clockwise)

Preceding gene: 119775152

Following gene: 119775154

Centisome position: 56.97

GC content: 52.73

Gene sequence:

>1722_bases
ATGGAAAAGCTTTCCGGCGCCAGCATGATTGTTCGATCCCTTATCGATGAAGGCGTAAAACACATTTTCGGTTATCCAGG
TGGTTCTGTACTGGATATCTACGATGCCCTCCATGAAAGTTCAAATATAGAGCACATTCTGGTTCGCCATGAACAGGCCG
CGGTACACATGGCCGACGGCTATGCCCGTGCCACAGGTGATGTGGGCGTAGTACTGGTAACCTCAGGCCCCGGCGCCACC
AACGCCATCACAGGCATTGCCACGGCGTATATGGACTCGGTCCCGCTGGTTATCCTGTCCGGCCAGGTGCCTTCCAGTCT
GATTGGCAACGATGCCTTTCAAGAATGCGACATGATTGGCATCTCAAGACCCATAGTGAAACACTCGTTTTTGGTAAAAG
ACCCCACTGAAATACCCGAAACCATTAAAAAAGCCTTTTACATTGCCGCTACCGGCCGCCCAGGCCCTGTGGTTGTGGAC
TTGCCCAAAGACACCCTGAACCCGGCGCTCAAGTTCGACTATGAGTACCCGTCAGAAGTGAAAATGCGCTCTTACAACCC
CACGGTGACAGGTCATAAAGGCCAAATCCGCCGGGGTTTGCAGGCCCTCCTCAATGCCAAAAAACCGGTACTGTATGTGG
GCGGCGGCGCTATTATCTCAAGCTGCGACAAGCAAATTCTGGCACTGTCTGAGCGTTTGAATATTCCAGTGGTCAACACC
CTGATGGGCCTTGGCGCCTTTCCCGGCACCCACCCCAACAACATAGGTATGCTGGGCATGCATGGCACCTATGAAGCCAA
CATGACCATGCATAACAGTGATCTGATTTTTGGTATTGGGGTACGTTTTGATGACCGAACCACCAATAACGTGCAGAAAT
ACTGCCCCAACGCCACAGTGCTGCATATCGATATCGACCCTTCATCCATCTCCAAGACGGTACAGGCTCACATCCCCATC
GTAGGCAGCGCCGACAAGGTACTGGATGAGATGCTGTCGCTGCTGGACGGCGACACCGAAATGCGAAACGATGAAGGCGC
CATCGACTGCTGGTGGAATGACATTAATCAGTGGCGTTCACGCAAGTGCCTGGCCTATGAGACCAACAGTGAAAAAATCA
AACCGCAGCAAGTCATAGAAACCCTCTATCGCCTCACCAAGGGCGATGCCTATGTGAGTTCCGATGTGGGACAGCATCAA
ATGTTTGCGGCCCTGTACTACCCCTTTGACAAGCCAAGACGCTGGATAAACTCTGGTGGTCTGGGCACCATGGGCTTTGG
TTTGCCCGCCGCCATGGGGGTGAAGATGGCGATGCCCGATGAAACCGTAGTCTGTGTCACCGGCGATGGCTCTATCCAGA
TGAATATTCAAGAGCTTTCTACCGCACTGCAATACGATGTGCCGGTAAAAATCATCAACCTTAACAACCGCTTCCTGGGT
ATGGTAAAGCAGTGGCAGGACATGATTTATTCAGGTCGCCACTCCCACTCCTACATGGACTCAGTACCGGACTTTGCCAA
GATTGCCGAGGCCTATGGCCATGTTGGCATTACCATCAGCCGCCCGGAGGAGCTTGAGCCCAAGCTCGCCGAAGCCCTGT
CGATGACTGACAGACTGGTGTTTGTAGACATTAATGTCGATGAAACTGAGCACGTTTACCCCATGCAAATTCGCGGGGGC
GCGATGAATGACATGTGGCTGAGCAAAACGGAGAGAAGCTGA

Upstream 100 bases:

>100_bases
TGAGCGAGTTTCTGGTACCCCAGGAGCTTGGTTTTAGAGCGATTACCGTCGCTGTAACAAGTACTGTTTACCGTAACGGT
ATCTTTTTGAGGATGCACGA

Downstream 100 bases:

>100_bases
TGCGCCGAATTATATCTGTACTTTTGGAAAACCAACCCGGTGCCCTTTCCCGTGTCGTTGGTCTGTTTTCCCAGCGTGGC
TATAACATCGAATGTCTGAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHESSNIEHILVRHEQAAVHMADGYARATGDVGVVLVTSGPGAT
NAITGIATAYMDSVPLVILSGQVPSSLIGNDAFQECDMIGISRPIVKHSFLVKDPTEIPETIKKAFYIAATGRPGPVVVD
LPKDTLNPALKFDYEYPSEVKMRSYNPTVTGHKGQIRRGLQALLNAKKPVLYVGGGAIISSCDKQILALSERLNIPVVNT
LMGLGAFPGTHPNNIGMLGMHGTYEANMTMHNSDLIFGIGVRFDDRTTNNVQKYCPNATVLHIDIDPSSISKTVQAHIPI
VGSADKVLDEMLSLLDGDTEMRNDEGAIDCWWNDINQWRSRKCLAYETNSEKIKPQQVIETLYRLTKGDAYVSSDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDVPVKIINLNNRFLG
MVKQWQDMIYSGRHSHSYMDSVPDFAKIAEAYGHVGITISRPEELEPKLAEALSMTDRLVFVDINVDETEHVYPMQIRGG
AMNDMWLSKTERS

Sequences:

>Translated_573_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHESSNIEHILVRHEQAAVHMADGYARATGDVGVVLVTSGPGAT
NAITGIATAYMDSVPLVILSGQVPSSLIGNDAFQECDMIGISRPIVKHSFLVKDPTEIPETIKKAFYIAATGRPGPVVVD
LPKDTLNPALKFDYEYPSEVKMRSYNPTVTGHKGQIRRGLQALLNAKKPVLYVGGGAIISSCDKQILALSERLNIPVVNT
LMGLGAFPGTHPNNIGMLGMHGTYEANMTMHNSDLIFGIGVRFDDRTTNNVQKYCPNATVLHIDIDPSSISKTVQAHIPI
VGSADKVLDEMLSLLDGDTEMRNDEGAIDCWWNDINQWRSRKCLAYETNSEKIKPQQVIETLYRLTKGDAYVSSDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDVPVKIINLNNRFLG
MVKQWQDMIYSGRHSHSYMDSVPDFAKIAEAYGHVGITISRPEELEPKLAEALSMTDRLVFVDINVDETEHVYPMQIRGG
AMNDMWLSKTERS
>Mature_573_residues
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHESSNIEHILVRHEQAAVHMADGYARATGDVGVVLVTSGPGAT
NAITGIATAYMDSVPLVILSGQVPSSLIGNDAFQECDMIGISRPIVKHSFLVKDPTEIPETIKKAFYIAATGRPGPVVVD
LPKDTLNPALKFDYEYPSEVKMRSYNPTVTGHKGQIRRGLQALLNAKKPVLYVGGGAIISSCDKQILALSERLNIPVVNT
LMGLGAFPGTHPNNIGMLGMHGTYEANMTMHNSDLIFGIGVRFDDRTTNNVQKYCPNATVLHIDIDPSSISKTVQAHIPI
VGSADKVLDEMLSLLDGDTEMRNDEGAIDCWWNDINQWRSRKCLAYETNSEKIKPQQVIETLYRLTKGDAYVSSDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDVPVKIINLNNRFLG
MVKQWQDMIYSGRHSHSYMDSVPDFAKIAEAYGHVGITISRPEELEPKLAEALSMTDRLVFVDINVDETEHVYPMQIRGG
AMNDMWLSKTERS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=591, Percent_Identity=26.2267343485618, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI21361361, Length=559, Percent_Identity=24.3291592128801, Blast_Score=153, Evalue=4e-37,
Organism=Escherichia coli, GI87081685, Length=576, Percent_Identity=75.6944444444444, Blast_Score=926, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=38.7610619469027, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=32.6047358834244, Blast_Score=316, Evalue=3e-87,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=29.1071428571429, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788716, Length=581, Percent_Identity=23.5800344234079, Blast_Score=160, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=588, Percent_Identity=25.3401360544218, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=588, Percent_Identity=25.3401360544218, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17542570, Length=518, Percent_Identity=25.6756756756757, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=42.6369863013699, Blast_Score=447, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=24.2616033755274, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=513, Percent_Identity=22.2222222222222, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=541, Percent_Identity=22.365988909427, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=516, Percent_Identity=23.062015503876, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=495, Percent_Identity=23.030303030303, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922626, Length=599, Percent_Identity=22.3706176961603, Blast_Score=166, Evalue=5e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62929; Mature: 62929

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHESSNIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCC
YARATGDVGVVLVTSGPGATNAITGIATAYMDSVPLVILSGQVPSSLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHHCCCC
ISRPIVKHSFLVKDPTEIPETIKKAFYIAATGRPGPVVVDLPKDTLNPALKFDYEYPSEV
CCCCHHHHEEEECCCHHHHHHHHHEEEEEECCCCCCEEEECCHHHCCCCEEECCCCCCCC
KMRSYNPTVTGHKGQIRRGLQALLNAKKPVLYVGGGAIISSCDKQILALSERLNIPVVNT
EEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPGTHPNNIGMLGMHGTYEANMTMHNSDLIFGIGVRFDDRTTNNVQKYCPNATV
HHHCCCCCCCCCCCEEEEECCCEEEECEEEECCCEEEEEEEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTVQAHIPIVGSADKVLDEMLSLLDGDTEMRNDEGAIDCWWNDINQWRS
EEEECCHHHHHHHHHEECCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHC
RKCLAYETNSEKIKPQQVIETLYRLTKGDAYVSSDVGQHQMFAALYYPFDKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHEEEEEECCCHHHHHHHCCCC
LGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDVPVKIINLNNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHH
MVKQWQDMIYSGRHSHSYMDSVPDFAKIAEAYGHVGITISRPEELEPKLAEALSMTDRLV
HHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCEEEEECCCHHCCHHHHHHHHHCCEEE
FVDINVDETEHVYPMQIRGGAMNDMWLSKTERS
EEEECCCCCCCEEEEEECCCCCCCHHCCCCCCC
>Mature Secondary Structure
MEKLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHESSNIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCEEEECCC
YARATGDVGVVLVTSGPGATNAITGIATAYMDSVPLVILSGQVPSSLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHHHHCCCC
ISRPIVKHSFLVKDPTEIPETIKKAFYIAATGRPGPVVVDLPKDTLNPALKFDYEYPSEV
CCCCHHHHEEEECCCHHHHHHHHHEEEEEECCCCCCEEEECCHHHCCCCEEECCCCCCCC
KMRSYNPTVTGHKGQIRRGLQALLNAKKPVLYVGGGAIISSCDKQILALSERLNIPVVNT
EEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHH
LMGLGAFPGTHPNNIGMLGMHGTYEANMTMHNSDLIFGIGVRFDDRTTNNVQKYCPNATV
HHHCCCCCCCCCCCEEEEECCCEEEECEEEECCCEEEEEEEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTVQAHIPIVGSADKVLDEMLSLLDGDTEMRNDEGAIDCWWNDINQWRS
EEEECCHHHHHHHHHEECCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHC
RKCLAYETNSEKIKPQQVIETLYRLTKGDAYVSSDVGQHQMFAALYYPFDKPRRWINSGG
CCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHEEEEEECCCHHHHHHHCCCC
LGTMGFGLPAAMGVKMAMPDETVVCVTGDGSIQMNIQELSTALQYDVPVKIINLNNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHH
MVKQWQDMIYSGRHSHSYMDSVPDFAKIAEAYGHVGITISRPEELEPKLAEALSMTDRLV
HHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCEEEEECCCHHCCHHHHHHHHHCCEEE
FVDINVDETEHVYPMQIRGGAMNDMWLSKTERS
EEEECCCCCCCEEEEEECCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]