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The map label for this gene is insF1 [C]

Identifier: 119775013

GI number: 119775013

Start: 2293542

End: 2294381

Strand: Direct

Name: insF1 [C]

Synonym: Sama_1878

Alternate gene names: 119775013

Gene position: 2293542-2294381 (Clockwise)

Preceding gene: 119775012

Following gene: 119775016

Centisome position: 53.26

GC content: 46.9

Gene sequence:

>840_bases
GTGCGGCAACTGTGTCAGCTGTTTGATGTTCATCCGAGCGGGTATTACGCGTGGAGAAGTTGCGCCAAGTCGAAGCGACA
ACGCGATAATGAACGACTTACAGGCCAACTCAAACAATGTTGGCTTGAGTCTGGTGGCGTCTATGGATATCGCAAACTCC
ATCGAGATCTACGTGATCTTGGTGAGCAATGCGGTATTAATCGCGTATATCGCCTGATGCAGCGAGCAGGATTGCGAGCA
CAAGTTGGTTATCGCAAGCCTAGAGCTCGTAGTGGTGAGCCGCATGTTGTAACGCCAAATCGTTTAGAACGGCAATTTAA
CCCCTTAGCGCCAAACCAAGCGTGGGTGACGGATATTACGTACATCAAAACGCATGAAGGTTGGCTCTATCTTGGCGCAG
TGATGGACTTGTTCTCACGTCACATTATTGGTTGGTCAATGGGCAGTCGAATCACCAAAGAACTTGCGTTAGATGCCTTA
TTGATGGCGGTTTGGCGTCGTAAACCTGATGGAAATGTCCTGGTTCACTCAGATCAAGGAAGCCAATACACGAGCCATGA
TTGGAATGAATTTCTAAGCGCTCATGGTTTGGAAGGTAGCATGAGCCGTCGGGGCAACTGCCATGATAACGCAGTAGCGG
AGAGTTTCTTTCAACTGCTGAAACGTGAACGAATTAGGCGAAAAATCTATGCGACTAGGAACGAGGCAAAGATGGATGTG
TTTAATTATATTGAGATGTTTTACAACCCGAAACGACAGCATAGTTCCAATGATGGGCTATCACCGTTAGAGTATGAGCG
TAAGTATTTTAATGAGGCTAAAAGTCGTCTAGTGAAGTAG

Upstream 100 bases:

>100_bases
TTACTGAAGAACGTGACCTGTTAAAAAAAGCCGCGGCGTACTTCGCAAGCCACCCCGAGTAAGATACGCCTTTATCCAAG
AGCATCATCACAGCCACTCT

Downstream 100 bases:

>100_bases
TGGCGATTCCCCCCCTCAAGTAATGCACCTCGGCCACAATCTGCCTTGGGTCAGAGAAAGGTCACGGTCACAAACCGCGA
TATGAGGAAGTGGAAAGCAT

Product: IS3 family transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MRQLCQLFDVHPSGYYAWRSCAKSKRQRDNERLTGQLKQCWLESGGVYGYRKLHRDLRDLGEQCGINRVYRLMQRAGLRA
QVGYRKPRARSGEPHVVTPNRLERQFNPLAPNQAWVTDITYIKTHEGWLYLGAVMDLFSRHIIGWSMGSRITKELALDAL
LMAVWRRKPDGNVLVHSDQGSQYTSHDWNEFLSAHGLEGSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRNEAKMDV
FNYIEMFYNPKRQHSSNDGLSPLEYERKYFNEAKSRLVK

Sequences:

>Translated_279_residues
MRQLCQLFDVHPSGYYAWRSCAKSKRQRDNERLTGQLKQCWLESGGVYGYRKLHRDLRDLGEQCGINRVYRLMQRAGLRA
QVGYRKPRARSGEPHVVTPNRLERQFNPLAPNQAWVTDITYIKTHEGWLYLGAVMDLFSRHIIGWSMGSRITKELALDAL
LMAVWRRKPDGNVLVHSDQGSQYTSHDWNEFLSAHGLEGSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRNEAKMDV
FNYIEMFYNPKRQHSSNDGLSPLEYERKYFNEAKSRLVK
>Mature_279_residues
MRQLCQLFDVHPSGYYAWRSCAKSKRQRDNERLTGQLKQCWLESGGVYGYRKLHRDLRDLGEQCGINRVYRLMQRAGLRA
QVGYRKPRARSGEPHVVTPNRLERQFNPLAPNQAWVTDITYIKTHEGWLYLGAVMDLFSRHIIGWSMGSRITKELALDAL
LMAVWRRKPDGNVLVHSDQGSQYTSHDWNEFLSAHGLEGSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRNEAKMDV
FNYIEMFYNPKRQHSSNDGLSPLEYERKYFNEAKSRLVK

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=231, Percent_Identity=36.3636363636364, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1788405, Length=271, Percent_Identity=42.4354243542435, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1787263, Length=271, Percent_Identity=42.4354243542435, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786753, Length=271, Percent_Identity=42.4354243542435, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786570, Length=271, Percent_Identity=42.4354243542435, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786490, Length=271, Percent_Identity=42.4354243542435, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789981, Length=251, Percent_Identity=35.0597609561753, Blast_Score=151, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32734; Mature: 32734

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLCQLFDVHPSGYYAWRSCAKSKRQRDNERLTGQLKQCWLESGGVYGYRKLHRDLRDL
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GEQCGINRVYRLMQRAGLRAQVGYRKPRARSGEPHVVTPNRLERQFNPLAPNQAWVTDIT
HHHCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEECCHHHHHHCCCCCCCCHHHHEEE
YIKTHEGWLYLGAVMDLFSRHIIGWSMGSRITKELALDALLMAVWRRKPDGNVLVHSDQG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
SQYTSHDWNEFLSAHGLEGSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRNEAKMDV
CCCCCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FNYIEMFYNPKRQHSSNDGLSPLEYERKYFNEAKSRLVK
HHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRQLCQLFDVHPSGYYAWRSCAKSKRQRDNERLTGQLKQCWLESGGVYGYRKLHRDLRDL
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GEQCGINRVYRLMQRAGLRAQVGYRKPRARSGEPHVVTPNRLERQFNPLAPNQAWVTDIT
HHHCCHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCEECCHHHHHHCCCCCCCCHHHHEEE
YIKTHEGWLYLGAVMDLFSRHIIGWSMGSRITKELALDALLMAVWRRKPDGNVLVHSDQG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
SQYTSHDWNEFLSAHGLEGSMSRRGNCHDNAVAESFFQLLKRERIRRKIYATRNEAKMDV
CCCCCCCHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
FNYIEMFYNPKRQHSSNDGLSPLEYERKYFNEAKSRLVK
HHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]