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The map label for this gene is astE

Identifier: 119774844

GI number: 119774844

Start: 2087535

End: 2088581

Strand: Direct

Name: astE

Synonym: Sama_1708

Alternate gene names: 119774844

Gene position: 2087535-2088581 (Clockwise)

Preceding gene: 119774842

Following gene: 119774845

Centisome position: 48.48

GC content: 53.1

Gene sequence:

>1047_bases
GTGCTTAACCTACTGCAAGGCTGCAAAGATTTTTTGCATCTTACCCTGTCCAATCCCGAACACCTTGACCCCATCGCCCC
TCAACCTCTGTACGGACACACCTTGGTCAGCCTCTGGGATACAGGCGTGTTGGTATTCGAGCCTGTCGATGGCAACAGCC
ATAAAGACATAGTGCTATCAAGCGGCGTGCATGGAAATGAAACCGCGCCTATTGAACTCTGTAATGGCCTTATTCAGGAT
GTGCTGGAAGGACGTTTACAGGTCAAAGAGCGGGTATTGTTCCTCATTGGTAACCCTGCTGCCATCAACAATGGCACCCG
TATCGTAGATGAAAACATGAACCGCCTTTTCAGCGGTGAGCACAGCCGTGGGCCCGGACTCAGTAACCCCGAGCGGGTGC
GGGCTAAAAAGCTTGAAACCTATGTCACCCGTTTCTTTGAAAAAGGTGCGGAAAAAGGTGAGGGCAGGCAGCGTATTCAT
TACGATTTACACACGGCCATCCGCGGCTCTAAGCATGAAAAGTTTGCTATCTACCCTTACCGCCCCGGCCGCGCCTTCAG
CGGTGAGCAAATCATGTTCCTTGCGGCGAGTGGCGTTGATACCGTGCTCTTTCACCACGAACCGACCACGACCTTCAGTT
ATTTCTCCTCCGAGTTGTTCCGTGCCGACGCCTTTACCATAGAACTTGGCAAGGTCTACCCCATGGGGCAGAACGACATG
AGTAAGTTCGCCAATACCCGGGAGATGTTCAAGCGCCTGATTTGCGCTGAGCCACTGGAGCTTGCACCTTTTGATGAAAC
CCAGGTGAACCTCTATCAGGTGTGCAGGGTTATCAATAAGGAAGTCGATGACTTTGAATTTACCTTCAGCACTGATGTCG
AAAACTTCAGCGCTTTCCCGCGTGGCCACGTGATAGCACGTCAGGGCGGCAAGGATATTCTTGTCGAGCAGGAAGCCGAG
GCGGTGGTGTTCCCCAACGCCAAGGTACCCGTGGGGCAGCGCACCGTCATCATGCTGGTTCCCGCGGTAAATCCTCACGT
GGAATAG

Upstream 100 bases:

>100_bases
AAAACTGCATGGCCAAAAGGATTGTCGCCCATCGGATGTGTGACTACTCTGGTCAACAGGCAGTTCTCAGCGAGCATAGC
CTCAGAGAAAGGAGACCCAA

Downstream 100 bases:

>100_bases
GGCTGTAAAGCAATTAATACATGCAGGCGGGGGAAACTTGTCTCCCTTCATAGTGTAATGATTGTCAGGTAGTTTACGTT
AACGTAATGTTCATCCCGCG

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLNLLQGCKDFLHLTLSNPEHLDPIAPQPLYGHTLVSLWDTGVLVFEPVDGNSHKDIVLSSGVHGNETAPIELCNGLIQD
VLEGRLQVKERVLFLIGNPAAINNGTRIVDENMNRLFSGEHSRGPGLSNPERVRAKKLETYVTRFFEKGAEKGEGRQRIH
YDLHTAIRGSKHEKFAIYPYRPGRAFSGEQIMFLAASGVDTVLFHHEPTTTFSYFSSELFRADAFTIELGKVYPMGQNDM
SKFANTREMFKRLICAEPLELAPFDETQVNLYQVCRVINKEVDDFEFTFSTDVENFSAFPRGHVIARQGGKDILVEQEAE
AVVFPNAKVPVGQRTVIMLVPAVNPHVE

Sequences:

>Translated_348_residues
MLNLLQGCKDFLHLTLSNPEHLDPIAPQPLYGHTLVSLWDTGVLVFEPVDGNSHKDIVLSSGVHGNETAPIELCNGLIQD
VLEGRLQVKERVLFLIGNPAAINNGTRIVDENMNRLFSGEHSRGPGLSNPERVRAKKLETYVTRFFEKGAEKGEGRQRIH
YDLHTAIRGSKHEKFAIYPYRPGRAFSGEQIMFLAASGVDTVLFHHEPTTTFSYFSSELFRADAFTIELGKVYPMGQNDM
SKFANTREMFKRLICAEPLELAPFDETQVNLYQVCRVINKEVDDFEFTFSTDVENFSAFPRGHVIARQGGKDILVEQEAE
AVVFPNAKVPVGQRTVIMLVPAVNPHVE
>Mature_348_residues
MLNLLQGCKDFLHLTLSNPEHLDPIAPQPLYGHTLVSLWDTGVLVFEPVDGNSHKDIVLSSGVHGNETAPIELCNGLIQD
VLEGRLQVKERVLFLIGNPAAINNGTRIVDENMNRLFSGEHSRGPGLSNPERVRAKKLETYVTRFFEKGAEKGEGRQRIH
YDLHTAIRGSKHEKFAIYPYRPGRAFSGEQIMFLAASGVDTVLFHHEPTTTFSYFSSELFRADAFTIELGKVYPMGQNDM
SKFANTREMFKRLICAEPLELAPFDETQVNLYQVCRVINKEVDDFEFTFSTDVENFSAFPRGHVIARQGGKDILVEQEAE
AVVFPNAKVPVGQRTVIMLVPAVNPHVE

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily

Homologues:

Organism=Escherichia coli, GI1788040, Length=330, Percent_Identity=34.8484848484849, Blast_Score=188, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASTE_SHEAM (A1S6A8)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927584.1
- ProteinModelPortal:   A1S6A8
- SMR:   A1S6A8
- STRING:   A1S6A8
- GeneID:   4603959
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1708
- NMPDR:   fig|326297.7.peg.1616
- eggNOG:   COG2988
- HOGENOM:   HBG298271
- OMA:   RWRWLGH
- PhylomeDB:   A1S6A8
- ProtClustDB:   PRK05324
- HAMAP:   MF_00767
- InterPro:   IPR007036
- InterPro:   IPR016681
- PIRSF:   PIRSF017020
- TIGRFAMs:   TIGR03242

Pfam domain/function: PF04952 AstE_AspA

EC number: =3.5.1.96

Molecular weight: Translated: 39041; Mature: 39041

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: ACT_SITE 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLLQGCKDFLHLTLSNPEHLDPIAPQPLYGHTLVSLWDTGVLVFEPVDGNSHKDIVLS
CCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEE
SGVHGNETAPIELCNGLIQDVLEGRLQVKERVLFLIGNPAAINNGTRIVDENMNRLFSGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCEEHHHHHHHHHCCC
HSRGPGLSNPERVRAKKLETYVTRFFEKGAEKGEGRQRIHYDLHTAIRGSKHEKFAIYPY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEEEC
RPGRAFSGEQIMFLAASGVDTVLFHHEPTTTFSYFSSELFRADAFTIELGKVYPMGQNDM
CCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCEEECCCHHH
SKFANTREMFKRLICAEPLELAPFDETQVNLYQVCRVINKEVDDFEFTFSTDVENFSAFP
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCC
RGHVIARQGGKDILVEQEAEAVVFPNAKVPVGQRTVIMLVPAVNPHVE
CCCEEEECCCCEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLNLLQGCKDFLHLTLSNPEHLDPIAPQPLYGHTLVSLWDTGVLVFEPVDGNSHKDIVLS
CCHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCEEEE
SGVHGNETAPIELCNGLIQDVLEGRLQVKERVLFLIGNPAAINNGTRIVDENMNRLFSGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCEEHHHHHHHHHCCC
HSRGPGLSNPERVRAKKLETYVTRFFEKGAEKGEGRQRIHYDLHTAIRGSKHEKFAIYPY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCEEEEEEC
RPGRAFSGEQIMFLAASGVDTVLFHHEPTTTFSYFSSELFRADAFTIELGKVYPMGQNDM
CCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCEEECCCHHH
SKFANTREMFKRLICAEPLELAPFDETQVNLYQVCRVINKEVDDFEFTFSTDVENFSAFP
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCC
RGHVIARQGGKDILVEQEAEAVVFPNAKVPVGQRTVIMLVPAVNPHVE
CCCEEEECCCCEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA