Click here to switch to the map view.

The map label for this gene is aglA [H]

Identifier: 119774798

GI number: 119774798

Start: 2033633

End: 2035267

Strand: Direct

Name: aglA [H]

Synonym: Sama_1662

Alternate gene names: 119774798

Gene position: 2033633-2035267 (Clockwise)

Preceding gene: 119774794

Following gene: 119774799

Centisome position: 47.23

GC content: 55.11

Gene sequence:

>1635_bases
ATGAGCATTCATCCCTGGTGGCGCGGCGCTGCCATCTATCAGATTTACCCGCGCAGTCTTAAGGACAGTAACGGCGATGG
TATTGGCGATCTGCAAGGGATCATCGAGCGTCTGGACTATATTGCCAGCCTCAATGTGGATGCGATTTGGATCTCGCCCT
TCTTCCGCTCTCCCATGAAGGACTTTGGTTACGATATCAGTGACTATCTTGATGTGGACCCCATGTTTGGCACCATGGAC
GACTTTGATGAGCTTATTGCCAAGGCCCATTCTCTGGGGTTGAAGGTGGTTATCGATCAGGTACTCAGCCACACTTCCGA
CGAACATGCCTGGTTTGCCGAGAGCCGCCAGAGCCGCGACAATCCAAAGGCCGACTGGTATGTGTGGGCCGATCCCCGAG
AAGACGGTACCCCTCCCAACAATTGGCTGGCGATATTCGGTGGCTGCGCCTGGGAATGGGAGCCAAGACGCCAGCAGTAT
TACCTGCACAATTTTTTGAAGAGCCAGCCGGATCTTAACTTTCACTGTGAAGCCGTTCGTCAGGCGGTGCTCAATAACGT
AGAGTTCTGGCTCAAAAAGGGCGTGGATGGTTTCCGGCTCGATGCCATTACTTTTTGTTTCCACGATAAGGCGCTTCGTG
ACAACCCGGCGAAACCGGCCGATAAGCGCCAGGGCCGAGGTTTTTCTGAAGACAACCCCTACGCCTATCAGTATCACTGG
TACAACAACGAACGTCCCGAAACCCTTGGTTTTATCGAAGATTTAAGAGCGCTGATTGATCGCTACCCGGGTGCCGTGAC
CCTCGGTGAAGTCTCAAGCGAAGACTCCCTTGCCACCATGGCCGAATACACCCGGGGCGATAACCGCCTGCACATGGCCT
ACAGCTTTGAGCTTTTAACCAAGGACTACAGCGCTGGGTATATCCGCAGCACAGTTGAAGCACTCGAAGGTGCCATCGGT
GACGGCTGGCCGTGCTGGGCCATAGGCAACCACGATGTGGAGCGGGTCATCAGTCGATGGGGGCAGGGTAAGGGCACACC
TCAAATGGCCAAGATGCTGTCGGCCATGCTGGGATGCCTGCGGGGCAGCCTGTGCGTGTATCAGGGCGAAGAGTTGGGGC
TTACCGAAGCCGACATCCCCTTTGATGCGCTGCAGGACCCCTTTGGTATCGCATTCTGGCCTAACTTTAAAGGCCGCGAT
GGCTGTCGTACCCCCATGCCCTGGCAAGCCGAAGGCGAATACATGGGATTCGGTGCGACAGCCTCTGGTGCCATGCCCTG
GTTACCGCTGCCAGAAGAACATCGTGCACTGAACGTTGCCGCCCAGAGTGCGAACCCGGACTCGGTGCTCAATCAGTTCA
GCCGCTTTATGGCATGGCGCCGCACTCAGCCGGCTTTGGTAACTGGTGAAATTAAATTTATTGATAGCAAAGAGCCCGTT
CTGGCGTTTGAACGTGTGAGCTCTGATAAAAAAGTGCTTTGTGTGTTCAATTTATCTGATAAGCCGCAAACGATGACTGT
TCCTGGCAGTGAGCCATGGAAGCTCCTTGAAGGCCATGGGCTTGAAGGTGGTAGTCTGGACGGCGCTGGCACTGTTCGCC
TTGCACCCCACGGGGCTCTGTTACTGGGCAACTAA

Upstream 100 bases:

>100_bases
CATCTTGGCTGTACTTACGCATTGGCAACAAAACGCCAACAGCCAGGGGCGTTACAAAGCCGGAAAACACGGCCTAAATG
ACCTGAATTGAGGGACAACA

Downstream 100 bases:

>100_bases
ATCCGAGAGAGTGGGATTGGCCTGTAAGACATTGGGTTCGGTGTCATTTGTGGAAGTTTGGTTTAGCTTTGTGTGCAACC
GATTAGAGTACAGTTGGGCG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSIHPWWRGAAIYQIYPRSLKDSNGDGIGDLQGIIERLDYIASLNVDAIWISPFFRSPMKDFGYDISDYLDVDPMFGTMD
DFDELIAKAHSLGLKVVIDQVLSHTSDEHAWFAESRQSRDNPKADWYVWADPREDGTPPNNWLAIFGGCAWEWEPRRQQY
YLHNFLKSQPDLNFHCEAVRQAVLNNVEFWLKKGVDGFRLDAITFCFHDKALRDNPAKPADKRQGRGFSEDNPYAYQYHW
YNNERPETLGFIEDLRALIDRYPGAVTLGEVSSEDSLATMAEYTRGDNRLHMAYSFELLTKDYSAGYIRSTVEALEGAIG
DGWPCWAIGNHDVERVISRWGQGKGTPQMAKMLSAMLGCLRGSLCVYQGEELGLTEADIPFDALQDPFGIAFWPNFKGRD
GCRTPMPWQAEGEYMGFGATASGAMPWLPLPEEHRALNVAAQSANPDSVLNQFSRFMAWRRTQPALVTGEIKFIDSKEPV
LAFERVSSDKKVLCVFNLSDKPQTMTVPGSEPWKLLEGHGLEGGSLDGAGTVRLAPHGALLLGN

Sequences:

>Translated_544_residues
MSIHPWWRGAAIYQIYPRSLKDSNGDGIGDLQGIIERLDYIASLNVDAIWISPFFRSPMKDFGYDISDYLDVDPMFGTMD
DFDELIAKAHSLGLKVVIDQVLSHTSDEHAWFAESRQSRDNPKADWYVWADPREDGTPPNNWLAIFGGCAWEWEPRRQQY
YLHNFLKSQPDLNFHCEAVRQAVLNNVEFWLKKGVDGFRLDAITFCFHDKALRDNPAKPADKRQGRGFSEDNPYAYQYHW
YNNERPETLGFIEDLRALIDRYPGAVTLGEVSSEDSLATMAEYTRGDNRLHMAYSFELLTKDYSAGYIRSTVEALEGAIG
DGWPCWAIGNHDVERVISRWGQGKGTPQMAKMLSAMLGCLRGSLCVYQGEELGLTEADIPFDALQDPFGIAFWPNFKGRD
GCRTPMPWQAEGEYMGFGATASGAMPWLPLPEEHRALNVAAQSANPDSVLNQFSRFMAWRRTQPALVTGEIKFIDSKEPV
LAFERVSSDKKVLCVFNLSDKPQTMTVPGSEPWKLLEGHGLEGGSLDGAGTVRLAPHGALLLGN
>Mature_543_residues
SIHPWWRGAAIYQIYPRSLKDSNGDGIGDLQGIIERLDYIASLNVDAIWISPFFRSPMKDFGYDISDYLDVDPMFGTMDD
FDELIAKAHSLGLKVVIDQVLSHTSDEHAWFAESRQSRDNPKADWYVWADPREDGTPPNNWLAIFGGCAWEWEPRRQQYY
LHNFLKSQPDLNFHCEAVRQAVLNNVEFWLKKGVDGFRLDAITFCFHDKALRDNPAKPADKRQGRGFSEDNPYAYQYHWY
NNERPETLGFIEDLRALIDRYPGAVTLGEVSSEDSLATMAEYTRGDNRLHMAYSFELLTKDYSAGYIRSTVEALEGAIGD
GWPCWAIGNHDVERVISRWGQGKGTPQMAKMLSAMLGCLRGSLCVYQGEELGLTEADIPFDALQDPFGIAFWPNFKGRDG
CRTPMPWQAEGEYMGFGATASGAMPWLPLPEEHRALNVAAQSANPDSVLNQFSRFMAWRRTQPALVTGEIKFIDSKEPVL
AFERVSSDKKVLCVFNLSDKPQTMTVPGSEPWKLLEGHGLEGGSLDGAGTVRLAPHGALLLGN

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=560, Percent_Identity=31.9642857142857, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI65506891, Length=108, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI61744475, Length=108, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744479, Length=110, Percent_Identity=32.7272727272727, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI61744481, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI61744483, Length=110, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI61744477, Length=108, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790687, Length=532, Percent_Identity=34.5864661654135, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1786604, Length=521, Percent_Identity=22.8406909788868, Blast_Score=102, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI25147709, Length=491, Percent_Identity=24.6435845213849, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565753, Length=532, Percent_Identity=23.3082706766917, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322245, Length=557, Percent_Identity=32.6750448833034, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6321731, Length=559, Percent_Identity=34.7048300536673, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6319776, Length=559, Percent_Identity=34.7048300536673, Blast_Score=276, Evalue=7e-75,
Organism=Saccharomyces cerevisiae, GI6321726, Length=566, Percent_Identity=32.5088339222615, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6322241, Length=566, Percent_Identity=33.3922261484099, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322021, Length=566, Percent_Identity=33.3922261484099, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6324416, Length=566, Percent_Identity=33.3922261484099, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24586591, Length=564, Percent_Identity=35.4609929078014, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24586593, Length=567, Percent_Identity=34.3915343915344, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24583747, Length=542, Percent_Identity=36.90036900369, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24583745, Length=570, Percent_Identity=35.4385964912281, Blast_Score=312, Evalue=4e-85,
Organism=Drosophila melanogaster, GI24583749, Length=542, Percent_Identity=36.90036900369, Blast_Score=312, Evalue=5e-85,
Organism=Drosophila melanogaster, GI24586587, Length=575, Percent_Identity=35.8260869565217, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI221330053, Length=572, Percent_Identity=34.0909090909091, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24586599, Length=520, Percent_Identity=34.6153846153846, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24586597, Length=522, Percent_Identity=36.2068965517241, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45549022, Length=522, Percent_Identity=35.632183908046, Blast_Score=295, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24586589, Length=525, Percent_Identity=34.0952380952381, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI281360393, Length=514, Percent_Identity=31.3229571984436, Blast_Score=238, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61204; Mature: 61073

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHPWWRGAAIYQIYPRSLKDSNGDGIGDLQGIIERLDYIASLNVDAIWISPFFRSPMK
CCCCCCCCCCEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHCCHHH
DFGYDISDYLDVDPMFGTMDDFDELIAKAHSLGLKVVIDQVLSHTSDEHAWFAESRQSRD
HHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCC
NPKADWYVWADPREDGTPPNNWLAIFGGCAWEWEPRRQQYYLHNFLKSQPDLNFHCEAVR
CCCCCEEEEECCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHH
QAVLNNVEFWLKKGVDGFRLDAITFCFHDKALRDNPAKPADKRQGRGFSEDNPYAYQYHW
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHCCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNERPETLGFIEDLRALIDRYPGAVTLGEVSSEDSLATMAEYTRGDNRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEC
KDYSAGYIRSTVEALEGAIGDGWPCWAIGNHDVERVISRWGQGKGTPQMAKMLSAMLGCL
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGSLCVYQGEELGLTEADIPFDALQDPFGIAFWPNFKGRDGCRTPMPWQAEGEYMGFGAT
CCCEEEEECCCCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCC
ASGAMPWLPLPEEHRALNVAAQSANPDSVLNQFSRFMAWRRTQPALVTGEIKFIDSKEPV
CCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCE
LAFERVSSDKKVLCVFNLSDKPQTMTVPGSEPWKLLEGHGLEGGSLDGAGTVRLAPHGAL
EEHHHCCCCCEEEEEEECCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCEE
LLGN
EECC
>Mature Secondary Structure 
SIHPWWRGAAIYQIYPRSLKDSNGDGIGDLQGIIERLDYIASLNVDAIWISPFFRSPMK
CCCCCCCCCEEEEEECHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECHHHCCHHH
DFGYDISDYLDVDPMFGTMDDFDELIAKAHSLGLKVVIDQVLSHTSDEHAWFAESRQSRD
HHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHCCC
NPKADWYVWADPREDGTPPNNWLAIFGGCAWEWEPRRQQYYLHNFLKSQPDLNFHCEAVR
CCCCCEEEEECCCCCCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHH
QAVLNNVEFWLKKGVDGFRLDAITFCFHDKALRDNPAKPADKRQGRGFSEDNPYAYQYHW
HHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHCCCCCCCCHHHCCCCCCCCCCEEEEEEE
YNNERPETLGFIEDLRALIDRYPGAVTLGEVSSEDSLATMAEYTRGDNRLHMAYSFELLT
ECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEC
KDYSAGYIRSTVEALEGAIGDGWPCWAIGNHDVERVISRWGQGKGTPQMAKMLSAMLGCL
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RGSLCVYQGEELGLTEADIPFDALQDPFGIAFWPNFKGRDGCRTPMPWQAEGEYMGFGAT
CCCEEEEECCCCCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEECCCC
ASGAMPWLPLPEEHRALNVAAQSANPDSVLNQFSRFMAWRRTQPALVTGEIKFIDSKEPV
CCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCE
LAFERVSSDKKVLCVFNLSDKPQTMTVPGSEPWKLLEGHGLEGGSLDGAGTVRLAPHGAL
EEHHHCCCCCEEEEEEECCCCCCEEECCCCCCCHHHCCCCCCCCCCCCCCEEEECCCCEE
LLGN
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]