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The map label for this gene is napB [H]

Identifier: 119774760

GI number: 119774760

Start: 1988307

End: 1988780

Strand: Direct

Name: napB [H]

Synonym: Sama_1623

Alternate gene names: 119774760

Gene position: 1988307-1988780 (Clockwise)

Preceding gene: 119774759

Following gene: 119774761

Centisome position: 46.17

GC content: 53.38

Gene sequence:

>474_bases
ATGAAAACAATGAAAACACTCTCTGCGCTTGCTCTGGTTGCCTTCGCCTGTCTGGCCAATGCCAGCACAGTCACAGATGA
GAAAATTGCCACCTTGCGTCAGGCGCCGCTGAATGTGGAAGTGACACCGCCAGCCATGCAGCAGGTCATGAACAAAGACG
TAAAGCAGGCCCGTAACTACCCGATGCAGCCCCCCGTCATTCCCCACAAGATTGATGGGTATCAGCTGGATCTGAAGGTA
AACAAGTGTATGTCGTGCCACGCCCGTACCCGTACCGGTGAGTCTCAGGCTCCCATGGTCAGCGTGACTCACTACATGGA
CAGGGATAATAACTTCCTGGCCGATCTGTCACCACGCCGTTATTTCTGCGTGCAGTGCCATGTGCCTCAGCTGGATGCCA
AGCCACTGGTTGACAACGAGTTTATTGACATTGATCACCTGCTCAAGGCGGATCAGGTCAAGGCTAAACACTAG

Upstream 100 bases:

>100_bases
TCATCAACAAGGTCACCCTGGATGCGACAGATCCCCTGTCCAAACAGACCGACTTCAAGAAGTGTGCTGTCAAAGTGTTG
AAGGCTTGAGGAGACAAACC

Downstream 100 bases:

>100_bases
GAGTCACTATGTGGGAAAAGCTTAAAAAAGTATGGCGAACCCTCAGGAAGCCCAGTGTGCATTACAGCCTTGGTTTCCTG
ACCCTTGGAGGCTTTGTGGC

Product: nitrate reductase cytochrome c-type subunit (NapB)

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MKTMKTLSALALVAFACLANASTVTDEKIATLRQAPLNVEVTPPAMQQVMNKDVKQARNYPMQPPVIPHKIDGYQLDLKV
NKCMSCHARTRTGESQAPMVSVTHYMDRDNNFLADLSPRRYFCVQCHVPQLDAKPLVDNEFIDIDHLLKADQVKAKH

Sequences:

>Translated_157_residues
MKTMKTLSALALVAFACLANASTVTDEKIATLRQAPLNVEVTPPAMQQVMNKDVKQARNYPMQPPVIPHKIDGYQLDLKV
NKCMSCHARTRTGESQAPMVSVTHYMDRDNNFLADLSPRRYFCVQCHVPQLDAKPLVDNEFIDIDHLLKADQVKAKH
>Mature_157_residues
MKTMKTLSALALVAFACLANASTVTDEKIATLRQAPLNVEVTPPAMQQVMNKDVKQARNYPMQPPVIPHKIDGYQLDLKV
NKCMSCHARTRTGESQAPMVSVTHYMDRDNNFLADLSPRRYFCVQCHVPQLDAKPLVDNEFIDIDHLLKADQVKAKH

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=83, Percent_Identity=49.3975903614458, Blast_Score=103, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 17661; Mature: 17661

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMKTLSALALVAFACLANASTVTDEKIATLRQAPLNVEVTPPAMQQVMNKDVKQARNY
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCC
PMQPPVIPHKIDGYQLDLKVNKCMSCHARTRTGESQAPMVSVTHYMDRDNNFLADLSPRR
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEHHHHCCCCCEEECCCCCE
YFCVQCHVPQLDAKPLVDNEFIDIDHLLKADQVKAKH
EEEEEECCCCCCCCCCCCCCEECHHHHHHHCHHCCCC
>Mature Secondary Structure
MKTMKTLSALALVAFACLANASTVTDEKIATLRQAPLNVEVTPPAMQQVMNKDVKQARNY
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCC
PMQPPVIPHKIDGYQLDLKVNKCMSCHARTRTGESQAPMVSVTHYMDRDNNFLADLSPRR
CCCCCCCCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCEEEEHHHHCCCCCEEECCCCCE
YFCVQCHVPQLDAKPLVDNEFIDIDHLLKADQVKAKH
EEEEEECCCCCCCCCCCCCCEECHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]