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The map label for this gene is napA [H]

Identifier: 119774759

GI number: 119774759

Start: 1985800

End: 1988295

Strand: Direct

Name: napA [H]

Synonym: Sama_1622

Alternate gene names: 119774759

Gene position: 1985800-1988295 (Clockwise)

Preceding gene: 119774758

Following gene: 119774760

Centisome position: 46.12

GC content: 55.73

Gene sequence:

>2496_bases
ATGAAAATGAACAGACGTGAATTTATGAAGGCCAATGCCGCACTGGCAGCGGCCAGTGTGGCCGGGCTGGCCTTGCCGAC
TTCGGCAAGTAATCTCATCACCAGCTCAGAGCAAACCAAGCTGGATTGGAACAAGGCGCCTTGCCGCTTCTGCGGTACCG
GCTGCTCAGTGATGGTGGCCACCCGTGACGGACGCGTCGTGGCGACCCACGGCGATGCCAACAGCGAAGTTAACCGCGGC
CTCAACTGCATCAAGGGCTACTTCCTGTCCAAAATCATGTACGGTAAAGACCGCCTGCAAACTCCCATGCTGCGTATGAC
CAACGGTCAATACGACAAGCATGGCGAATTTACGCCCATCAGCTGGGACAGCGCCTTCGATATCATGGCCGAAAAGTGGA
AAGCCACCATCAAAGCCAAGGGCCCGACTGCCATCGGTATGTTCGGTTCAGGCCAGTGGACCGTGTGGGAAGGCTATGCC
GCCTCCAAACTGATGAAAGCCGGTTTCGGGTCAAACAACATCGACCCCAATGCCCGTCACTGTATGGCCTCTGCCGTAGT
GGGCTTTATGCGCACCTTCGGTATGGATGAGCCCATGGGCTGTTACAACGATATGGAAGCTGCCGACGCCTTTGTGCTGT
GGGGCTCCAACATGGCCGAAATGCACCCCATTTTGTGGAGCCGCGTGACAGACCGTCGCCTGAGCGCGCCCCACGTTAAA
GTTGCTGTGCTGTCTACCTTTGAGCACCGCTCCTTTGAACTTGCCGACTTGCCTGTGGTCTTCACACCACAATCCGATCT
GGCCATTCTCAACTTTATCGCCAACTACATCATCCAAAACGACATGGTGAACCATGACTTCGTCAAGAAGCATGTGAACT
TCCGTCAGGGTGTGACTGACATTGGTTACGGTCTGCGTCCGACCCACCCATTGCAGCAAAAGGCGAAAAACGTTGCCACG
GCAGGGGATTCGAGCCCCATCGATTTCGCTGCGTTCAAGGACTTCGTGTCAGAGTACACGTTGGAAAAAACGGCCCAGAT
GTCCGGCGTTGCTGAAGACAAGCTGGTGGAACTTGCCAAGTTGTATGCCGATCCCAACACCAAGGTCACGTCTTTCTGGA
CCATGGGTGTCAACCAGCATACCCGCGGCGTGTGGTGTAACAACCTGATTTACAACATCCATCTGCTCACAGGCAAGATC
TCTACTCCCGGTAACAGCCCCTTCTCGCTGACCGGTCAACCCTCAGCCTGTGGTACGGCCCGCGAAGTGGGTACCTTTGC
CCACCGTCTGCCAGCCGACATGGTGGTGATGAACCCTGAGCACCGTGCCTACGCTGAAAAAGTGTGGAAGATCCCTGCCG
GTATCATTCCGCCCAAGCCTGGTTATCACGCGGTTGAACAAAACCGCCGCCTGAAAGACGGTGATCTCAACGTCTACTGG
GTGCAGGTAAACAACAACATGCAGGCTGCGCCTAACATGATGGAAGAAACCTTGCCCGGTTACCGTAATCCAGACAACTT
CATCGTTGTGTCGGATGCGTACCCAACCGTGACCACCCAGGCGGCCGACCTGATTCTGCCTACTGCCATGTGGGTGGAAA
AAGAGGGTGCCTACGGTAACGCTGAACGTCGGACCCAGTTCTGGCACCAGCTGGTCAAAGCGCCGGGCGAGTCACGCTCA
GATCTGTGGCAGTTGATGGAGTTCTCCAAGCGCTTTACCACCGACGAAGTCTGGCCAAAAGACGTGCTGGCGGCGAATCC
GTCTTACAAGGGCAAAACCCTGTATGACGTGCTCTACAAGAATGGTCATGTAGACAAATTCCCGCTCACCGATCGCGATG
CCAAGTACGCCAACGATGAAGCCGAGTACTTTGGTTTCTACGTACAGAAGGGGTTGTTTGAAGAGTACGCCGAGTTTGGC
CGCGGTCACGGACACGATTTGGCTCCCTTCGACATGTACCACGAAGCCCATGGTCTGCGTTGGCCTGTCGTCGACGGCAA
AGAAACCCTGTGGCGCTACCGCGAAGGTTCAGACCCTTATGTCAAGCCTGGCAAGGGCTTTGAGTTCTACGGTAAGCCCG
ATGGCAAAGCGGTGATTTTCGCGCTGCCCTACGAGCCACCTGCAGAGTCGCCCGATGCTGAATACGATATGTGGCTCTCA
ACCGGCCGTGTACTTGAACATTGGCACTCAGGCTCCATGACCCAGCGTGTACCAGAGCTTTATCGCGCCTTCCCGGATGC
CGTGTGTTTTATGCACCCTGACGATGCCAAGGCCCGTGGTTTACGCCGCGGCGATGAGGTCAAGGTCGTGTCCCGTCGTG
GTGAAATTCTTACCCGTGTCGAGACCCGTGGCCGTAACAAGCCGCCAAAAGGCCTGGTGTTTGTTCCCTGGTTTGATGCC
AGTCAGCTCATCAACAAGGTCACCCTGGATGCGACAGATCCCCTGTCCAAACAGACCGACTTCAAGAAGTGTGCTGTCAA
AGTGTTGAAGGCTTGA

Upstream 100 bases:

>100_bases
AAGAATTGCTGGCCGGAATCGAAGCCATTAACGCCCTGCCGGGCGTGATGTCGGCCACCATGGTTTATCACCAGAGCGAA
GTGCTGGAAGAGGATGAGCA

Downstream 100 bases:

>100_bases
GGAGACAAACCATGAAAACAATGAAAACACTCTCTGCGCTTGCTCTGGTTGCCTTCGCCTGTCTGGCCAATGCCAGCACA
GTCACAGATGAGAAAATTGC

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 831; Mature: 831

Protein sequence:

>831_residues
MKMNRREFMKANAALAAASVAGLALPTSASNLITSSEQTKLDWNKAPCRFCGTGCSVMVATRDGRVVATHGDANSEVNRG
LNCIKGYFLSKIMYGKDRLQTPMLRMTNGQYDKHGEFTPISWDSAFDIMAEKWKATIKAKGPTAIGMFGSGQWTVWEGYA
ASKLMKAGFGSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYNDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVK
VAVLSTFEHRSFELADLPVVFTPQSDLAILNFIANYIIQNDMVNHDFVKKHVNFRQGVTDIGYGLRPTHPLQQKAKNVAT
AGDSSPIDFAAFKDFVSEYTLEKTAQMSGVAEDKLVELAKLYADPNTKVTSFWTMGVNQHTRGVWCNNLIYNIHLLTGKI
STPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPEHRAYAEKVWKIPAGIIPPKPGYHAVEQNRRLKDGDLNVYW
VQVNNNMQAAPNMMEETLPGYRNPDNFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQLVKAPGESRS
DLWQLMEFSKRFTTDEVWPKDVLAANPSYKGKTLYDVLYKNGHVDKFPLTDRDAKYANDEAEYFGFYVQKGLFEEYAEFG
RGHGHDLAPFDMYHEAHGLRWPVVDGKETLWRYREGSDPYVKPGKGFEFYGKPDGKAVIFALPYEPPAESPDAEYDMWLS
TGRVLEHWHSGSMTQRVPELYRAFPDAVCFMHPDDAKARGLRRGDEVKVVSRRGEILTRVETRGRNKPPKGLVFVPWFDA
SQLINKVTLDATDPLSKQTDFKKCAVKVLKA

Sequences:

>Translated_831_residues
MKMNRREFMKANAALAAASVAGLALPTSASNLITSSEQTKLDWNKAPCRFCGTGCSVMVATRDGRVVATHGDANSEVNRG
LNCIKGYFLSKIMYGKDRLQTPMLRMTNGQYDKHGEFTPISWDSAFDIMAEKWKATIKAKGPTAIGMFGSGQWTVWEGYA
ASKLMKAGFGSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYNDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVK
VAVLSTFEHRSFELADLPVVFTPQSDLAILNFIANYIIQNDMVNHDFVKKHVNFRQGVTDIGYGLRPTHPLQQKAKNVAT
AGDSSPIDFAAFKDFVSEYTLEKTAQMSGVAEDKLVELAKLYADPNTKVTSFWTMGVNQHTRGVWCNNLIYNIHLLTGKI
STPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPEHRAYAEKVWKIPAGIIPPKPGYHAVEQNRRLKDGDLNVYW
VQVNNNMQAAPNMMEETLPGYRNPDNFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQLVKAPGESRS
DLWQLMEFSKRFTTDEVWPKDVLAANPSYKGKTLYDVLYKNGHVDKFPLTDRDAKYANDEAEYFGFYVQKGLFEEYAEFG
RGHGHDLAPFDMYHEAHGLRWPVVDGKETLWRYREGSDPYVKPGKGFEFYGKPDGKAVIFALPYEPPAESPDAEYDMWLS
TGRVLEHWHSGSMTQRVPELYRAFPDAVCFMHPDDAKARGLRRGDEVKVVSRRGEILTRVETRGRNKPPKGLVFVPWFDA
SQLINKVTLDATDPLSKQTDFKKCAVKVLKA
>Mature_831_residues
MKMNRREFMKANAALAAASVAGLALPTSASNLITSSEQTKLDWNKAPCRFCGTGCSVMVATRDGRVVATHGDANSEVNRG
LNCIKGYFLSKIMYGKDRLQTPMLRMTNGQYDKHGEFTPISWDSAFDIMAEKWKATIKAKGPTAIGMFGSGQWTVWEGYA
ASKLMKAGFGSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYNDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVK
VAVLSTFEHRSFELADLPVVFTPQSDLAILNFIANYIIQNDMVNHDFVKKHVNFRQGVTDIGYGLRPTHPLQQKAKNVAT
AGDSSPIDFAAFKDFVSEYTLEKTAQMSGVAEDKLVELAKLYADPNTKVTSFWTMGVNQHTRGVWCNNLIYNIHLLTGKI
STPGNSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNPEHRAYAEKVWKIPAGIIPPKPGYHAVEQNRRLKDGDLNVYW
VQVNNNMQAAPNMMEETLPGYRNPDNFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGNAERRTQFWHQLVKAPGESRS
DLWQLMEFSKRFTTDEVWPKDVLAANPSYKGKTLYDVLYKNGHVDKFPLTDRDAKYANDEAEYFGFYVQKGLFEEYAEFG
RGHGHDLAPFDMYHEAHGLRWPVVDGKETLWRYREGSDPYVKPGKGFEFYGKPDGKAVIFALPYEPPAESPDAEYDMWLS
TGRVLEHWHSGSMTQRVPELYRAFPDAVCFMHPDDAKARGLRRGDEVKVVSRRGEILTRVETRGRNKPPKGLVFVPWFDA
SQLINKVTLDATDPLSKQTDFKKCAVKVLKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=72.289156626506, Blast_Score=1283, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=802, Percent_Identity=24.6882793017456, Blast_Score=219, Evalue=8e-58,
Organism=Escherichia coli, GI3868720, Length=663, Percent_Identity=22.3227752639517, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI3868719, Length=479, Percent_Identity=22.5469728601253, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87081797, Length=890, Percent_Identity=22.8089887640449, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93168; Mature: 93168

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNRREFMKANAALAAASVAGLALPTSASNLITSSEQTKLDWNKAPCRFCGTGCSVMVA
CCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHCCCCCCCCCCCCCCCCCEECCCCEEEEE
TRDGRVVATHGDANSEVNRGLNCIKGYFLSKIMYGKDRLQTPMLRMTNGQYDKHGEFTPI
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHEECCCCCCCCCCCCCC
SWDSAFDIMAEKWKATIKAKGPTAIGMFGSGQWTVWEGYAASKLMKAGFGSNNIDPNARH
CCCHHHHHHHHHHHEEEEECCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVVGFMRTFGMDEPMGCYNDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVK
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEE
VAVLSTFEHRSFELADLPVVFTPQSDLAILNFIANYIIQNDMVNHDFVKKHVNFRQGVTD
EEEEEHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHH
IGYGLRPTHPLQQKAKNVATAGDSSPIDFAAFKDFVSEYTLEKTAQMSGVAEDKLVELAK
HCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LYADPNTKVTSFWTMGVNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTA
HHCCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCH
REVGTFAHRLPADMVVMNPEHRAYAEKVWKIPAGIIPPKPGYHAVEQNRRLKDGDLNVYW
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEE
VQVNNNMQAAPNMMEETLPGYRNPDNFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGN
EEECCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCC
AERRTQFWHQLVKAPGESRSDLWQLMEFSKRFTTDEVWPKDVLAANPSYKGKTLYDVLYK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHC
NGHVDKFPLTDRDAKYANDEAEYFGFYVQKGLFEEYAEFGRGHGHDLAPFDMYHEAHGLR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
WPVVDGKETLWRYREGSDPYVKPGKGFEFYGKPDGKAVIFALPYEPPAESPDAEYDMWLS
CCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
TGRVLEHWHSGSMTQRVPELYRAFPDAVCFMHPDDAKARGLRRGDEVKVVSRRGEILTRV
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCEEEEECCCHHHHHH
ETRGRNKPPKGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKVLKA
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKMNRREFMKANAALAAASVAGLALPTSASNLITSSEQTKLDWNKAPCRFCGTGCSVMVA
CCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHCCCCCCCCCCCCCCCCCEECCCCEEEEE
TRDGRVVATHGDANSEVNRGLNCIKGYFLSKIMYGKDRLQTPMLRMTNGQYDKHGEFTPI
ECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHEECCCCCCCCCCCCCC
SWDSAFDIMAEKWKATIKAKGPTAIGMFGSGQWTVWEGYAASKLMKAGFGSNNIDPNARH
CCCHHHHHHHHHHHEEEEECCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVVGFMRTFGMDEPMGCYNDMEAADAFVLWGSNMAEMHPILWSRVTDRRLSAPHVK
HHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEE
VAVLSTFEHRSFELADLPVVFTPQSDLAILNFIANYIIQNDMVNHDFVKKHVNFRQGVTD
EEEEEHHCCCCEEEECCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHH
IGYGLRPTHPLQQKAKNVATAGDSSPIDFAAFKDFVSEYTLEKTAQMSGVAEDKLVELAK
HCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LYADPNTKVTSFWTMGVNQHTRGVWCNNLIYNIHLLTGKISTPGNSPFSLTGQPSACGTA
HHCCCCCCEEEEEEECCCCCCCCEEECCEEEEEEEEEEEECCCCCCCEEECCCCCCCCCH
REVGTFAHRLPADMVVMNPEHRAYAEKVWKIPAGIIPPKPGYHAVEQNRRLKDGDLNVYW
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEE
VQVNNNMQAAPNMMEETLPGYRNPDNFIVVSDAYPTVTTQAADLILPTAMWVEKEGAYGN
EEECCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCC
AERRTQFWHQLVKAPGESRSDLWQLMEFSKRFTTDEVWPKDVLAANPSYKGKTLYDVLYK
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHC
NGHVDKFPLTDRDAKYANDEAEYFGFYVQKGLFEEYAEFGRGHGHDLAPFDMYHEAHGLR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCC
WPVVDGKETLWRYREGSDPYVKPGKGFEFYGKPDGKAVIFALPYEPPAESPDAEYDMWLS
CCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHH
TGRVLEHWHSGSMTQRVPELYRAFPDAVCFMHPDDAKARGLRRGDEVKVVSRRGEILTRV
HHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCEEEEECCCHHHHHH
ETRGRNKPPKGLVFVPWFDASQLINKVTLDATDPLSKQTDFKKCAVKVLKA
HHCCCCCCCCCEEEEECCCHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA