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The map label for this gene is rhtC [H]

Identifier: 119774687

GI number: 119774687

Start: 1887305

End: 1887988

Strand: Reverse

Name: rhtC [H]

Synonym: Sama_1550

Alternate gene names: 119774687

Gene position: 1887988-1887305 (Counterclockwise)

Preceding gene: 119774688

Following gene: 119774684

Centisome position: 43.84

GC content: 55.41

Gene sequence:

>684_bases
ATGTTGGACTTTACCCTGCTTGGCAGCATTGCCGTCATTCATACCATCGCACTGGCAAGCCCGGGGCCGGACTTTGCCAT
TATGCTCAAGGTATCTCGCTATCAAACCCGCACGGTTGCCATCATGACCGCATTGGGCATTGCTGCGGCCATTTTGGCCC
ATACCCTTGCCAGCCTCACCGGCCTCAGCTTGCTTATCCACACTACCCCCTGGCTGTTTGGGACTGTGCAGGCGCTGGGC
GCCAGTTACCTGGGATATATGGGGATGGGCGCGTTGATGTCGGTGCGAAAGCAATTTTTATTGCGAAGGGAGCTGCCATT
TTCGGCGGAATCAACGCCAACGGATGTGCCACATAACCCAGACCCGGTGCTGAGCACAGCAAAGGGCTTTCGCCTGGGAC
TTTACACCAATTTATTGAATCCAAAGGCGTTGGTATTTTTCCTGACCTTGTTTTCTGCCATGGTGGGCCCCGACGTAAAC
GGCCCTACCCGCATCGCGTTGTTGGTGCTGATGTTTACCCTGTCCTTCGCCTGGTTTGGTCTGCTCGCCGTGCTGCTCAC
CAAAGGCAGCAGTCAACGCACGCTGGTGCGAATGGGGCCGATTATTGACCTGGTAACAGGAGTGCTCTTTTTGATGGTGA
GCGTGGCCATTCTCTACGGACTGGCCACCGAATATTGGGGGTAA

Upstream 100 bases:

>100_bases
TCAGCATGGCTTCAGCGACCTTAGCCACTTTACTCGCCACTTTCGCATGGCCTTTTTAACGTCACCCAGCGCCTATCGTC
GCGCTATCAGGAATTAACGG

Downstream 100 bases:

>100_bases
CACGGCACAGCTGCTTTCGTTTTCCGATGCCAGGATTCATAGTTCCCGAACCGAAACGTATCTTTGAGAAGCGAGCCGGG
TGTAAGCTAACCCGTCTCGC

Product: threonine efflux protein

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLDFTLLGSIAVIHTIALASPGPDFAIMLKVSRYQTRTVAIMTALGIAAAILAHTLASLTGLSLLIHTTPWLFGTVQALG
ASYLGYMGMGALMSVRKQFLLRRELPFSAESTPTDVPHNPDPVLSTAKGFRLGLYTNLLNPKALVFFLTLFSAMVGPDVN
GPTRIALLVLMFTLSFAWFGLLAVLLTKGSSQRTLVRMGPIIDLVTGVLFLMVSVAILYGLATEYWG

Sequences:

>Translated_227_residues
MLDFTLLGSIAVIHTIALASPGPDFAIMLKVSRYQTRTVAIMTALGIAAAILAHTLASLTGLSLLIHTTPWLFGTVQALG
ASYLGYMGMGALMSVRKQFLLRRELPFSAESTPTDVPHNPDPVLSTAKGFRLGLYTNLLNPKALVFFLTLFSAMVGPDVN
GPTRIALLVLMFTLSFAWFGLLAVLLTKGSSQRTLVRMGPIIDLVTGVLFLMVSVAILYGLATEYWG
>Mature_227_residues
MLDFTLLGSIAVIHTIALASPGPDFAIMLKVSRYQTRTVAIMTALGIAAAILAHTLASLTGLSLLIHTTPWLFGTVQALG
ASYLGYMGMGALMSVRKQFLLRRELPFSAESTPTDVPHNPDPVLSTAKGFRLGLYTNLLNPKALVFFLTLFSAMVGPDVN
GPTRIALLVLMFTLSFAWFGLLAVLLTKGSSQRTLVRMGPIIDLVTGVLFLMVSVAILYGLATEYWG

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=203, Percent_Identity=32.512315270936, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 24420; Mature: 24420

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFTLLGSIAVIHTIALASPGPDFAIMLKVSRYQTRTVAIMTALGIAAAILAHTLASLT
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLLIHTTPWLFGTVQALGASYLGYMGMGALMSVRKQFLLRRELPFSAESTPTDVPHNP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DPVLSTAKGFRLGLYTNLLNPKALVFFLTLFSAMVGPDVNGPTRIALLVLMFTLSFAWFG
CHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LLAVLLTKGSSQRTLVRMGPIIDLVTGVLFLMVSVAILYGLATEYWG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDFTLLGSIAVIHTIALASPGPDFAIMLKVSRYQTRTVAIMTALGIAAAILAHTLASLT
CCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLSLLIHTTPWLFGTVQALGASYLGYMGMGALMSVRKQFLLRRELPFSAESTPTDVPHNP
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
DPVLSTAKGFRLGLYTNLLNPKALVFFLTLFSAMVGPDVNGPTRIALLVLMFTLSFAWFG
CHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LLAVLLTKGSSQRTLVRMGPIIDLVTGVLFLMVSVAILYGLATEYWG
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]