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The map label for this gene is hemL [H]

Identifier: 119774619

GI number: 119774619

Start: 1794452

End: 1795843

Strand: Reverse

Name: hemL [H]

Synonym: Sama_1482

Alternate gene names: 119774619

Gene position: 1795843-1794452 (Counterclockwise)

Preceding gene: 119774620

Following gene: 119774618

Centisome position: 41.7

GC content: 58.84

Gene sequence:

>1392_bases
ATGACCGCGAGCGGGATCAGTTCACATGCCGTAGAGACTTTTATGAGCCATGAGCGAAGCCACTTTATCGAGCGAAACCC
CAAGTCCCGCAGTTTGGCCGAACGTGCGCAAAAATCTCTTTATGGCGGCGTCCCCATGCATTGGATGGCCGACTGGTCAA
CGCCCTTTCCGCTGTTTGTTGAAAGCGCCAACGGCGCCCGCTTCAGGGATGTGGATGGCCACGAATATGTGGACTTTTGC
CTGGGTGACACCGGCAGCATGTTCGGCCACTCGCCGGCACCTGTGGCCTCGGCCCTGGCACGCCAGTCGACCAAGGGGCT
CACCACCATGCTGCCCGGCGAAGATGCCATAGTGTGCGGCGAGATTTTGAGCGAGCGATTTGGTCTGCCCTACTGGCAGG
TGACCGCCACGGCCACAGATGCCAACCGTTACGTGGTGCGCTGGGCCCGCGCCATCACCCAAAAGCCGGTACTGCTGGTG
TTTGATGGCTGTTACCACGGCACTGTTGATGATGTCATGGTACGCACGCAGGGTGACCAAACCGTGCACCGCTCCGGCCT
GGTGGGCCAGGTATATGATTTAAGCCAGTACAGCCGCGCGATTCCGTTTAATGACACAGACGCGCTCGAACAGGCACTGA
AGCAAGGTGATGTGTGTGCCCTCTTGTGCGAACCGGCCATGACCAACATAGGGATGGTGATGCCGGATGAAGGCTTTATG
CAAAAGTGCCGTGAATTGACCCGTCAATACGGCGCCTTGTTCGTGGTTGACGAAACCCACACCATTTCCACCGACATAGG
TGGCTGTACTCGGCTGTGGCAACTTGACCCTGACTTTTTCGTGATGGGCAAACCCGTCGCCGGCGGCGTGCCCTGCGGCG
TATTCGGTTGCAGCGAGGCCATGAGTCAGGCCATGCAATCGGCCAAAGCGAGGATCAATCAGCAGTCCCACGGCCATGGT
CACAGCGGCATGGGCACCACACTGTCGGCCAATGCGCTCGCCATGCACTGTATGCGGGCCAATCTGGAAGACGTGGTCAA
TCAAAGTGCATACGACCATATGCTGCCATTGGCAAAACGCCTCGCTGCAGGCTTTCGCGATGTCATTGCTAAACACAAGC
TGCCCTGGTCTGTGACAGAGCTTGGCGCCCGCTGCGAATTTCAGTTCTGCCAAACACCGCCGCGCACCGGCGCCGAGGCC
GAAGCCGCCTTCCACGATGAGCTGCAGATGGCACTGCATTTGTACCTTATCAACCGCGGCATTCTGATCACCCCGTTCCA
CAACATGACCCTGTGCTGCCCCCACACCAGCGTTGCCGATGTGGACAAACTGGTCAGCACGCTCGATGAGGCCCTGACCA
GCCTGCTTGCCATCGAGGGGGCCAGGTTGTAA

Upstream 100 bases:

>100_bases
GGCCAACATGCGCCATCACTCGGTGGCTATTGCCAAGCCCCTCTGGCAATGATAATAATCAACACCGCGTTCAATAAAAC
AAACAACAAGGAGCCATCAC

Downstream 100 bases:

>100_bases
GCCACTGGGGTACTGTGGCAGCCATGCTGTCACAGTACCCTCACCACCGGGCTTGCACGGCTGAGTTTTTAGGCTGTAGC
TACCGGGGTCCATCATGCTA

Product: aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MTASGISSHAVETFMSHERSHFIERNPKSRSLAERAQKSLYGGVPMHWMADWSTPFPLFVESANGARFRDVDGHEYVDFC
LGDTGSMFGHSPAPVASALARQSTKGLTTMLPGEDAIVCGEILSERFGLPYWQVTATATDANRYVVRWARAITQKPVLLV
FDGCYHGTVDDVMVRTQGDQTVHRSGLVGQVYDLSQYSRAIPFNDTDALEQALKQGDVCALLCEPAMTNIGMVMPDEGFM
QKCRELTRQYGALFVVDETHTISTDIGGCTRLWQLDPDFFVMGKPVAGGVPCGVFGCSEAMSQAMQSAKARINQQSHGHG
HSGMGTTLSANALAMHCMRANLEDVVNQSAYDHMLPLAKRLAAGFRDVIAKHKLPWSVTELGARCEFQFCQTPPRTGAEA
EAAFHDELQMALHLYLINRGILITPFHNMTLCCPHTSVADVDKLVSTLDEALTSLLAIEGARL

Sequences:

>Translated_463_residues
MTASGISSHAVETFMSHERSHFIERNPKSRSLAERAQKSLYGGVPMHWMADWSTPFPLFVESANGARFRDVDGHEYVDFC
LGDTGSMFGHSPAPVASALARQSTKGLTTMLPGEDAIVCGEILSERFGLPYWQVTATATDANRYVVRWARAITQKPVLLV
FDGCYHGTVDDVMVRTQGDQTVHRSGLVGQVYDLSQYSRAIPFNDTDALEQALKQGDVCALLCEPAMTNIGMVMPDEGFM
QKCRELTRQYGALFVVDETHTISTDIGGCTRLWQLDPDFFVMGKPVAGGVPCGVFGCSEAMSQAMQSAKARINQQSHGHG
HSGMGTTLSANALAMHCMRANLEDVVNQSAYDHMLPLAKRLAAGFRDVIAKHKLPWSVTELGARCEFQFCQTPPRTGAEA
EAAFHDELQMALHLYLINRGILITPFHNMTLCCPHTSVADVDKLVSTLDEALTSLLAIEGARL
>Mature_462_residues
TASGISSHAVETFMSHERSHFIERNPKSRSLAERAQKSLYGGVPMHWMADWSTPFPLFVESANGARFRDVDGHEYVDFCL
GDTGSMFGHSPAPVASALARQSTKGLTTMLPGEDAIVCGEILSERFGLPYWQVTATATDANRYVVRWARAITQKPVLLVF
DGCYHGTVDDVMVRTQGDQTVHRSGLVGQVYDLSQYSRAIPFNDTDALEQALKQGDVCALLCEPAMTNIGMVMPDEGFMQ
KCRELTRQYGALFVVDETHTISTDIGGCTRLWQLDPDFFVMGKPVAGGVPCGVFGCSEAMSQAMQSAKARINQQSHGHGH
SGMGTTLSANALAMHCMRANLEDVVNQSAYDHMLPLAKRLAAGFRDVIAKHKLPWSVTELGARCEFQFCQTPPRTGAEAE
AAFHDELQMALHLYLINRGILITPFHNMTLCCPHTSVADVDKLVSTLDEALTSLLAIEGARL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=412, Percent_Identity=25.7281553398058, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789016, Length=358, Percent_Identity=24.5810055865922, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787560, Length=376, Percent_Identity=26.5957446808511, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788044, Length=271, Percent_Identity=23.9852398523985, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789759, Length=272, Percent_Identity=24.6323529411765, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144271, Length=313, Percent_Identity=20.7667731629393, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357415, Length=363, Percent_Identity=23.1404958677686, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 50696; Mature: 50565

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASGISSHAVETFMSHERSHFIERNPKSRSLAERAQKSLYGGVPMHWMADWSTPFPLFV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEE
ESANGARFRDVDGHEYVDFCLGDTGSMFGHSPAPVASALARQSTKGLTTMLPGEDAIVCG
ECCCCCEEECCCCCCEEHHEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHH
EILSERFGLPYWQVTATATDANRYVVRWARAITQKPVLLVFDGCYHGTVDDVMVRTQGDQ
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHEEEECCCCC
TVHRSGLVGQVYDLSQYSRAIPFNDTDALEQALKQGDVCALLCEPAMTNIGMVMPDEGFM
HHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCEECCCHHHH
QKCRELTRQYGALFVVDETHTISTDIGGCTRLWQLDPDFFVMGKPVAGGVPCGVFGCSEA
HHHHHHHHHHCEEEEEECCEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHH
MSQAMQSAKARINQQSHGHGHSGMGTTLSANALAMHCMRANLEDVVNQSAYDHMLPLAKR
HHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LAAGFRDVIAKHKLPWSVTELGARCEFQFCQTPPRTGAEAEAAFHDELQMALHLYLINRG
HHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ILITPFHNMTLCCPHTSVADVDKLVSTLDEALTSLLAIEGARL
EEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TASGISSHAVETFMSHERSHFIERNPKSRSLAERAQKSLYGGVPMHWMADWSTPFPLFV
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCEEE
ESANGARFRDVDGHEYVDFCLGDTGSMFGHSPAPVASALARQSTKGLTTMLPGEDAIVCG
ECCCCCEEECCCCCCEEHHEECCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCHHH
EILSERFGLPYWQVTATATDANRYVVRWARAITQKPVLLVFDGCYHGTVDDVMVRTQGDQ
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHEEEECCCCC
TVHRSGLVGQVYDLSQYSRAIPFNDTDALEQALKQGDVCALLCEPAMTNIGMVMPDEGFM
HHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECHHHHHCCEECCCHHHH
QKCRELTRQYGALFVVDETHTISTDIGGCTRLWQLDPDFFVMGKPVAGGVPCGVFGCSEA
HHHHHHHHHHCEEEEEECCEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCHHHHHHH
MSQAMQSAKARINQQSHGHGHSGMGTTLSANALAMHCMRANLEDVVNQSAYDHMLPLAKR
HHHHHHHHHHHHCCCCCCCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
LAAGFRDVIAKHKLPWSVTELGARCEFQFCQTPPRTGAEAEAAFHDELQMALHLYLINRG
HHHHHHHHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
ILITPFHNMTLCCPHTSVADVDKLVSTLDEALTSLLAIEGARL
EEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA