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The map label for this gene is vat [H]

Identifier: 119774521

GI number: 119774521

Start: 1676123

End: 1676803

Strand: Reverse

Name: vat [H]

Synonym: Sama_1384

Alternate gene names: 119774521

Gene position: 1676803-1676123 (Counterclockwise)

Preceding gene: 119774522

Following gene: 119774509

Centisome position: 38.94

GC content: 54.04

Gene sequence:

>681_bases
ATGACAAAACTCCCCCATACCACCGCCCATACCCAAGCGAAAACACAACCCGGCCCCAGCCCCGATAATCCATTCCCCAT
GGAAGGCTTCCCGCAGGTGTGCTTTATCAAAAACACCATCAGCAATCCCAACATTCAGGTGGGCGATTACAGCTATTACG
ACGACCCAGAGGACAGTGCCAACTTTGAGCACAACGTGCTGTATCACTTCCCCTTTATTGGCGACAAGCTGATTATCGGT
AAATTCTGCGCCATCGCCCGCGGGGTCAGGTTCATCATGAATGGTGCCAACCATCCCATGGCTGGCGTGAGCACCTATCC
GTTTTTTATTTTTGGCAACGGCTGGGAGCAAGCGGCGCCTGAGCCGGGGGAATTACCCTATAAAGGCGATACCGAAATCG
GCAACGATGTGTGGATAGGCTATGAGGCCCTGATTATGCCCGGGGTAAAGGTGGGCAATGGCGCCATTATCGCCAGCCGC
GCTGTGGTCACCACTGACGTACCTGCCTACGCCATCGTTGGTGGCAACCCAGCCAAGGTGATGAAAATGCGCTTTGATGA
GAAAGACATCGCCCTGCTTGAAGCCATCGCCTGGTGGGATTGGCCGGTAGCGAAAATTACCGCCCACCTGCTCGATATCA
AAAGTGGTAATCTTGAAGCCCTGAAGCAGGCACAACCGTAA

Upstream 100 bases:

>100_bases
ACATCCTGTACTTGAAGCTCGACTGAAAAAGGCGCCATGAGCCAGATCGCCAAATCATGTGCCAGCGTCTTGGCAAGACC
GTTTATCACAAGGATTGTTT

Downstream 100 bases:

>100_bases
TCACCTGGCCATGTTGAAACACCCGACCGGGGAAACTGTCCCTAAAACCGCATCGTTATTGTTTCTGGCTTTATTTGCGG
CTTTATTTGCGACTTTATTT

Product: streptogramin A acetyl transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTKLPHTTAHTQAKTQPGPSPDNPFPMEGFPQVCFIKNTISNPNIQVGDYSYYDDPEDSANFEHNVLYHFPFIGDKLIIG
KFCAIARGVRFIMNGANHPMAGVSTYPFFIFGNGWEQAAPEPGELPYKGDTEIGNDVWIGYEALIMPGVKVGNGAIIASR
AVVTTDVPAYAIVGGNPAKVMKMRFDEKDIALLEAIAWWDWPVAKITAHLLDIKSGNLEALKQAQP

Sequences:

>Translated_226_residues
MTKLPHTTAHTQAKTQPGPSPDNPFPMEGFPQVCFIKNTISNPNIQVGDYSYYDDPEDSANFEHNVLYHFPFIGDKLIIG
KFCAIARGVRFIMNGANHPMAGVSTYPFFIFGNGWEQAAPEPGELPYKGDTEIGNDVWIGYEALIMPGVKVGNGAIIASR
AVVTTDVPAYAIVGGNPAKVMKMRFDEKDIALLEAIAWWDWPVAKITAHLLDIKSGNLEALKQAQP
>Mature_225_residues
TKLPHTTAHTQAKTQPGPSPDNPFPMEGFPQVCFIKNTISNPNIQVGDYSYYDDPEDSANFEHNVLYHFPFIGDKLIIGK
FCAIARGVRFIMNGANHPMAGVSTYPFFIFGNGWEQAAPEPGELPYKGDTEIGNDVWIGYEALIMPGVKVGNGAIIASRA
VVTTDVPAYAIVGGNPAKVMKMRFDEKDIALLEAIAWWDWPVAKITAHLLDIKSGNLEALKQAQP

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=123, Percent_Identity=38.2113821138211, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 24778; Mature: 24647

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLPHTTAHTQAKTQPGPSPDNPFPMEGFPQVCFIKNTISNPNIQVGDYSYYDDPEDSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCC
NFEHNVLYHFPFIGDKLIIGKFCAIARGVRFIMNGANHPMAGVSTYPFFIFGNGWEQAAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCC
EPGELPYKGDTEIGNDVWIGYEALIMPGVKVGNGAIIASRAVVTTDVPAYAIVGGNPAKV
CCCCCCCCCCCCCCCEEEECEEEEEECCEEECCCEEEEECEEEEECCCEEEEECCCHHHE
MKMRFDEKDIALLEAIAWWDWPVAKITAHLLDIKSGNLEALKQAQP
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TKLPHTTAHTQAKTQPGPSPDNPFPMEGFPQVCFIKNTISNPNIQVGDYSYYDDPEDSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCCC
NFEHNVLYHFPFIGDKLIIGKFCAIARGVRFIMNGANHPMAGVSTYPFFIFGNGWEQAAP
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCHHHCCC
EPGELPYKGDTEIGNDVWIGYEALIMPGVKVGNGAIIASRAVVTTDVPAYAIVGGNPAKV
CCCCCCCCCCCCCCCEEEECEEEEEECCEEECCCEEEEECEEEEECCCEEEEECCCHHHE
MKMRFDEKDIALLEAIAWWDWPVAKITAHLLDIKSGNLEALKQAQP
EEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]