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The map label for this gene is paaF [C]

Identifier: 119774497

GI number: 119774497

Start: 1649591

End: 1650421

Strand: Reverse

Name: paaF [C]

Synonym: Sama_1360

Alternate gene names: 119774497

Gene position: 1650421-1649591 (Counterclockwise)

Preceding gene: 119774498

Following gene: 119774496

Centisome position: 38.33

GC content: 58.6

Gene sequence:

>831_bases
ATGACAACCATGACTTCCATTGCCGAGCTGTCTGCCTCCTTCAGCCGCATCCGCCTGGAGCTTAACGGCAGGGTGGGGTA
TCTCATCTTAAATCGCCCCGAGGTGCACAACGCCTTCGATGAGGTGATGATTAGCGAAATGACCCAGGCCATCAACGCCT
TCGCCCATGAGAGCGAGTGCGCTGTAATGGTGCTTAAAAGCGACGGCAAGCACTTCAGCGCTGGCGCCGACTTGAACTGG
ATGCGCAAGCAAGCCGCCATGGACTTTGACGACAACCTCAAAGATGCCCGCGAGCTTGCCAACCTGATGGATACCCTGGA
CCGCTTCCCCAAGCCCTCCATCGCCCTGGTAAACGGTGCAGCCTACGGCGGCGCGCTGGGGCTGGTTTGCTGCTGCGATA
TTGCGATTTCGAGCCACAAAGCGAGTTTCTGCCTGTCTGAAGTCAAACTTGGCCTCATTCCGGCGGTGATTAGCCCGTAC
GTGGTGCGCGCCATGGGACCACGCCAGAGCCGCCGCTATATGCTCACGGCTGAGCGCTTCGATGCCAGCATGGCGCTGGC
CTTAGGCGTAGTGCATGAAATCGCAGACGATTTGGACGCTGCAGCCGCGCCCATTATCCAAAATCTGCTGAGTGGCAGCC
CCCAGGCGCTGGGGTGGTGCAAATCGCTTATTGCCCGTTTGCAAAGCGGCGTAATTGATGAGCACACCCGTGACTACACC
AGCGAGCGCATCGCCCGCATTCGGGTGTCCGCCGAAGGACAGGAAGGCCTCAACGCCTTTTTTGATAAGCGCAGCCCAGC
GTGGGTTAATGATACCGGCAGCAAGGACTAA

Upstream 100 bases:

>100_bases
GCGCAAACCCGTGACGTACTGGGGCTGGCCATATCCGCCGCCCTCAATGCCCCCATCGAAGAGACCCGCTTCGGCGTGTT
CCGCATGTAAGGAGAAGGCT

Downstream 100 bases:

>100_bases
GGGATTTTTCATGTTTAACAAACTCTTAATTGCCAATCGCGGCGAAATTGCCTGCCGCGTTATCCGCACCGCCCGCGACA
TGGGCATTAAAACCGTGGCC

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MTTMTSIAELSASFSRIRLELNGRVGYLILNRPEVHNAFDEVMISEMTQAINAFAHESECAVMVLKSDGKHFSAGADLNW
MRKQAAMDFDDNLKDARELANLMDTLDRFPKPSIALVNGAAYGGALGLVCCCDIAISSHKASFCLSEVKLGLIPAVISPY
VVRAMGPRQSRRYMLTAERFDASMALALGVVHEIADDLDAAAAPIIQNLLSGSPQALGWCKSLIARLQSGVIDEHTRDYT
SERIARIRVSAEGQEGLNAFFDKRSPAWVNDTGSKD

Sequences:

>Translated_276_residues
MTTMTSIAELSASFSRIRLELNGRVGYLILNRPEVHNAFDEVMISEMTQAINAFAHESECAVMVLKSDGKHFSAGADLNW
MRKQAAMDFDDNLKDARELANLMDTLDRFPKPSIALVNGAAYGGALGLVCCCDIAISSHKASFCLSEVKLGLIPAVISPY
VVRAMGPRQSRRYMLTAERFDASMALALGVVHEIADDLDAAAAPIIQNLLSGSPQALGWCKSLIARLQSGVIDEHTRDYT
SERIARIRVSAEGQEGLNAFFDKRSPAWVNDTGSKD
>Mature_275_residues
TTMTSIAELSASFSRIRLELNGRVGYLILNRPEVHNAFDEVMISEMTQAINAFAHESECAVMVLKSDGKHFSAGADLNWM
RKQAAMDFDDNLKDARELANLMDTLDRFPKPSIALVNGAAYGGALGLVCCCDIAISSHKASFCLSEVKLGLIPAVISPYV
VRAMGPRQSRRYMLTAERFDASMALALGVVHEIADDLDAAAAPIIQNLLSGSPQALGWCKSLIARLQSGVIDEHTRDYTS
ERIARIRVSAEGQEGLNAFFDKRSPAWVNDTGSKD

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=29.2307692307692, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=25.8823529411765, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI213417737, Length=207, Percent_Identity=25.1207729468599, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI157694516, Length=207, Percent_Identity=25.1207729468599, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI70995211, Length=183, Percent_Identity=30.6010928961749, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI45643119, Length=189, Percent_Identity=27.5132275132275, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260275230, Length=189, Percent_Identity=27.5132275132275, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI260274832, Length=189, Percent_Identity=27.5132275132275, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787659, Length=251, Percent_Identity=29.8804780876494, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=27.6515151515151, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=25.3731343283582, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=28.4644194756554, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=29.3650793650794, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=190, Percent_Identity=31.0526315789474, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554946, Length=268, Percent_Identity=28.7313432835821, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=26.7441860465116, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=181, Percent_Identity=29.2817679558011, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=216, Percent_Identity=29.1666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=249, Percent_Identity=26.1044176706827, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=244, Percent_Identity=26.2295081967213, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=200, Percent_Identity=25, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=28.8973384030418, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=28.8973384030418, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=28.5140562248996, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920382, Length=219, Percent_Identity=28.310502283105, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30086; Mature: 29955

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMTSIAELSASFSRIRLELNGRVGYLILNRPEVHNAFDEVMISEMTQAINAFAHESEC
CCCHHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVMVLKSDGKHFSAGADLNWMRKQAAMDFDDNLKDARELANLMDTLDRFPKPSIALVNGA
EEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
AYGGALGLVCCCDIAISSHKASFCLSEVKLGLIPAVISPYVVRAMGPRQSRRYMLTAERF
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHH
DASMALALGVVHEIADDLDAAAAPIIQNLLSGSPQALGWCKSLIARLQSGVIDEHTRDYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
SERIARIRVSAEGQEGLNAFFDKRSPAWVNDTGSKD
HHHHHHEEECCCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTMTSIAELSASFSRIRLELNGRVGYLILNRPEVHNAFDEVMISEMTQAINAFAHESEC
CCHHHHHHHHHHHHHEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
AVMVLKSDGKHFSAGADLNWMRKQAAMDFDDNLKDARELANLMDTLDRFPKPSIALVNGA
EEEEEECCCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
AYGGALGLVCCCDIAISSHKASFCLSEVKLGLIPAVISPYVVRAMGPRQSRRYMLTAERF
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHH
DASMALALGVVHEIADDLDAAAAPIIQNLLSGSPQALGWCKSLIARLQSGVIDEHTRDYT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
SERIARIRVSAEGQEGLNAFFDKRSPAWVNDTGSKD
HHHHHHEEECCCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA