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The map label for this gene is pspA [H]

Identifier: 119774374

GI number: 119774374

Start: 1522209

End: 1522892

Strand: Direct

Name: pspA [H]

Synonym: Sama_1237

Alternate gene names: 119774374

Gene position: 1522209-1522892 (Clockwise)

Preceding gene: 119774365

Following gene: 119774375

Centisome position: 35.35

GC content: 51.46

Gene sequence:

>684_bases
ATGGGTATTTTCTCTCGTTTCGCGGACATCATTAACTCCAACATCAGTGCCTTGCTGGACAAGGCAGAAGATCCGGAAAA
AATGGTTCGCCTTATCATTCAGGAAATGGAAGACACCCTGGTGGAAGTGCGCTCTACCTCAGCCAAGGTGCTGGCCGAAA
AGAAAGACATCCTTCGCCGCATCGCCCGAGTTGATGCTCAGGTTCAGGATTGGGAAGAGAAAGCCGAGCTGGCCATCTCC
AAAGAGCGTGAAGATCTGGCCCGTGCTGCCCTGGTAGAAAAGCAAAAAGCCTCTACCCTGGCCGATACTCTGCAGCGCGA
GCTCGAAGTGGTCGAAGATCATATCCTGCGCCTGAAAGAAGAAGTAACTCAGCTTCAGGAAAAACTGGCCGATGCCAAGG
CACGCCAGAAGACCATCATCATGCGCAAGCAGACTGCTTCCAGCCGTCTTGAGGTAAAAAAGCAGCTGGATTCCAGTAAA
ATAGACAATGCCATGCTGAAGTTCGAGCAGTATGAACAACGTGTCGAGAGCCTGGAAGCTCAGGTGGAGTCATACGATCT
GGCCAACAAATCCAGTCTGAACGACGAGTTTGCCGCTCTGCAGGCCGAAGATTCAGTGAATGCCGAGCTGGAAGCCCTGA
AGGCGAAAATGAAGGGCAAGACCAAAGAAAAAGCTGGCAAGTAA

Upstream 100 bases:

>100_bases
TAAAATTTCAGCTTTGTTGTTGAAAAATATATAAAAACCAAAGTTGGCACGCTCTGTGTATTACTCATAGCGACAACCAA
CTATATGTGAGGATTGAATT

Downstream 100 bases:

>100_bases
CGAATTATTCGGGGGAGGTGAGTTATGGATATGGATGTATTGATGGCGCCCATTATTATTTTCATGGTGATAGTGGCACC
CATATGGCTGGTTCTGCATT

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKDILRRIARVDAQVQDWEEKAELAIS
KEREDLARAALVEKQKASTLADTLQRELEVVEDHILRLKEEVTQLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSK
IDNAMLKFEQYEQRVESLEAQVESYDLANKSSLNDEFAALQAEDSVNAELEALKAKMKGKTKEKAGK

Sequences:

>Translated_227_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKDILRRIARVDAQVQDWEEKAELAIS
KEREDLARAALVEKQKASTLADTLQRELEVVEDHILRLKEEVTQLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSK
IDNAMLKFEQYEQRVESLEAQVESYDLANKSSLNDEFAALQAEDSVNAELEALKAKMKGKTKEKAGK
>Mature_226_residues
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKDILRRIARVDAQVQDWEEKAELAISK
EREDLARAALVEKQKASTLADTLQRELEVVEDHILRLKEEVTQLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKI
DNAMLKFEQYEQRVESLEAQVESYDLANKSSLNDEFAALQAEDSVNAELEALKAKMKGKTKEKAGK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=218, Percent_Identity=55.9633027522936, Blast_Score=250, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25776; Mature: 25645

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKDILRR
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARVDAQVQDWEEKAELAISKEREDLARAALVEKQKASTLADTLQRELEVVEDHILRLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVTQLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKIDNAMLKFEQYEQRVESLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLANKSSLNDEFAALQAEDSVNAELEALKAKMKGKTKEKAGK
HHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKDILRR
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARVDAQVQDWEEKAELAISKEREDLARAALVEKQKASTLADTLQRELEVVEDHILRLKE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVTQLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKIDNAMLKFEQYEQRVESLEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVESYDLANKSSLNDEFAALQAEDSVNAELEALKAKMKGKTKEKAGK
HHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]