Click here to switch to the map view.

The map label for this gene is folD

Identifier: 119774359

GI number: 119774359

Start: 1503327

End: 1504181

Strand: Reverse

Name: folD

Synonym: Sama_1222

Alternate gene names: 119774359

Gene position: 1504181-1503327 (Counterclockwise)

Preceding gene: 119774366

Following gene: 119774357

Centisome position: 34.93

GC content: 56.61

Gene sequence:

>855_bases
ATGACAGCCCAAAATATCGATGGCAAGGCCATTGCCCGTGCCATTCGCACTACCCTCAGCGACAAGGTAAAAGCCCGTAA
AGAGGCCGGCCAGCGCATTCCGGGTCTTGCCGTAATCCTGGTGGGGTTGGATCCCGCCTCCCAGGTCTATGTTGGTTCCA
AGCGCAAGGCCTGCGAAGAGGTCGGATTCTTATCCCAGTCCTTTGACCTGCCCGACACCACCAGCGAGGACGATCTGCTG
GCGCTGATTGACCGCTGCAATGAAGACCCGGCCATCGATGGCATTCTGGTGCAATTGCCACTGCCTGCGCACATTGACTC
TTCCAAGGTGATTGAACGTATTCGCCCCGATAAAGATGTCGATGGTTTCCACCCTTATAATGTGGGCAGATTGGCACAGC
GCATTCCTGTGCTGCGCTCCTGCACCCCTATGGGCATCATGACCCTTATCCGCTCTACCGGCGTGGATACCTATGGTCTG
GATGCCGTCGTGGTTGGTGCCTCCAATATCGTGGGCCGCCCCATGACACTGGAGTTGCTGCTGGCAGGCTGCACCACCAC
CACCTGCCATCGCTTTACCAAAAACCTCGAAGATAAGGTGCGTCAGGCTGACTTGCTGGTTGTGGCCGTGGGTAAACCCC
ACTTTATTCCCGGCGACTGGATTAAGCCCGGTGCCATTGTGATTGACGTGGGTATCAACCGTCTCGATGACGGCCGCTTG
GTAGGGGATGTTGAATTCGACGTGGCGGCAGAACGTGCAGCCTTTATCACTCCAGTGCCTGGTGGCGTGGGGCCAATGAC
CATTGCGAGTCTGTTGGAAAACACCCTCTATGCCGCTGAGAACTATCACGACTGA

Upstream 100 bases:

>100_bases
CCAATGGGGACAATGACACTGTGGCCCTTGGCTGTTAGAATGTGCGCGCTAAAAACTTCAGGCTGGTTTACCACAGTCGA
ATACAGGAAGCCCTACACCC

Downstream 100 bases:

>100_bases
GTGGTGATACCTTTACTATGGCAAAAGTTGCCAGCGTAACGGCAGCAACATAACGATGTACCAATAAAAACGCCTGCAAT
TGCAGGCGTTTTTATTGGTA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTAQNIDGKAIARAIRTTLSDKVKARKEAGQRIPGLAVILVGLDPASQVYVGSKRKACEEVGFLSQSFDLPDTTSEDDLL
ALIDRCNEDPAIDGILVQLPLPAHIDSSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIRSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEDKVRQADLLVVAVGKPHFIPGDWIKPGAIVIDVGINRLDDGRL
VGDVEFDVAAERAAFITPVPGGVGPMTIASLLENTLYAAENYHD

Sequences:

>Translated_284_residues
MTAQNIDGKAIARAIRTTLSDKVKARKEAGQRIPGLAVILVGLDPASQVYVGSKRKACEEVGFLSQSFDLPDTTSEDDLL
ALIDRCNEDPAIDGILVQLPLPAHIDSSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIRSTGVDTYGL
DAVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEDKVRQADLLVVAVGKPHFIPGDWIKPGAIVIDVGINRLDDGRL
VGDVEFDVAAERAAFITPVPGGVGPMTIASLLENTLYAAENYHD
>Mature_283_residues
TAQNIDGKAIARAIRTTLSDKVKARKEAGQRIPGLAVILVGLDPASQVYVGSKRKACEEVGFLSQSFDLPDTTSEDDLLA
LIDRCNEDPAIDGILVQLPLPAHIDSSKVIERIRPDKDVDGFHPYNVGRLAQRIPVLRSCTPMGIMTLIRSTGVDTYGLD
AVVVGASNIVGRPMTLELLLAGCTTTTCHRFTKNLEDKVRQADLLVVAVGKPHFIPGDWIKPGAIVIDVGINRLDDGRLV
GDVEFDVAAERAAFITPVPGGVGPMTIASLLENTLYAAENYHD

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=298, Percent_Identity=45.9731543624161, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=42.6116838487972, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=42.9553264604811, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI36796743, Length=198, Percent_Identity=29.2929292929293, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=70.7746478873239, Blast_Score=416, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=42.1232876712329, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=41.4141414141414, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=284, Percent_Identity=42.9577464788732, Blast_Score=222, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=317, Percent_Identity=27.1293375394322, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=47.4747474747475, Blast_Score=253, Evalue=8e-68,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=47.4747474747475, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645718, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI17137370, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI62472483, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI45551871, Length=290, Percent_Identity=43.7931034482759, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_SHEAM (A1S4X3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927099.1
- ProteinModelPortal:   A1S4X3
- SMR:   A1S4X3
- STRING:   A1S4X3
- GeneID:   4603474
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1222
- NMPDR:   fig|326297.7.peg.1153
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   A1S4X3
- ProtClustDB:   PRK10792
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30562; Mature: 30430

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQNIDGKAIARAIRTTLSDKVKARKEAGQRIPGLAVILVGLDPASQVYVGSKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
VGFLSQSFDLPDTTSEDDLLALIDRCNEDPAIDGILVQLPLPAHIDSSKVIERIRPDKDV
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIRSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEDKVRQADLLVVAVGKPHFIPGDWIKPGAIVIDVGINRLDDGRL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCE
VGDVEFDVAAERAAFITPVPGGVGPMTIASLLENTLYAAENYHD
EEEEEHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TAQNIDGKAIARAIRTTLSDKVKARKEAGQRIPGLAVILVGLDPASQVYVGSKRKACEE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCHHHHHHH
VGFLSQSFDLPDTTSEDDLLALIDRCNEDPAIDGILVQLPLPAHIDSSKVIERIRPDKDV
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLAQRIPVLRSCTPMGIMTLIRSTGVDTYGLDAVVVGASNIVGRPMTLELL
CCCCCCCHHHHHHHCCHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECHHHCCCCCEEEHH
LAGCTTTTCHRFTKNLEDKVRQADLLVVAVGKPHFIPGDWIKPGAIVIDVGINRLDDGRL
HHCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCE
VGDVEFDVAAERAAFITPVPGGVGPMTIASLLENTLYAAENYHD
EEEEEHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA