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The map label for this gene is miaE [H]

Identifier: 119774355

GI number: 119774355

Start: 1499678

End: 1500448

Strand: Direct

Name: miaE [H]

Synonym: Sama_1218

Alternate gene names: 119774355

Gene position: 1499678-1500448 (Clockwise)

Preceding gene: 119774353

Following gene: 119774358

Centisome position: 34.83

GC content: 50.45

Gene sequence:

>771_bases
GTGATAGAGCAGTTGTTGGCGCCAGTCAGAGGATTTCTAGCCTGCGAAACTCCTGATGCCTGGATTGAGAAAGCCAAAGA
CCCCGGTGTGTTACAGTCGATTTTGGTTGACCATTGCAACTGCGAGCTGAAAGCCGCACAAACTGCCCTGTTCTTAATCC
GCAAATATGCGGTAGACCCTGAGTACAGTTCGGCGCTGCTTGCCTGGGCAAAGCCTTATGAAGAATTTGTCTATCACAAA
GACAGGGATATGAAGGCCTTCCTGGCCCGCACCGGGAAAAAGAACGAACTGGCTACAGAGCTTAAACCCAAATCAGGCGT
TGCATTTGGCGACGAACTAATCGCCAGAATGGTTCGCTTGATTAAGGAAGAGTTTCATCATTTCGAGCAGGTGCTTGAAA
TCATGCATGCCAGAGATATTCCCTATACCAATATCAACGCAGGACGCTATGCCAAGGGCATGATGAGTCATGTGCGTACC
AGTGAGCCTGCAACTCTTGTCGATAAGCTTATCGTAGGTGCCTTTATCGAGGCCCGCTCCTGTGAGCGTTTCGCCAAACT
CGCCCCCTTTATGGATGAAGAGCTTGAGCGATTTTACGTGTCGCTTTTACGTTCAGAGGCCCGGCATTATCAGGATTATT
TGACCCTTGCCGAGATGATTGCGGGGGAAAATATAGACCAGCGCATCGCTTACTTCGCCGGGGTGGAAGCGGCGTTGATT
CAAGGTGCAGATGACGAGTTTCGCTTTCACAGCGGGGTGCCGAATACCTGA

Upstream 100 bases:

>100_bases
GAACAAAAATTGACATCCGTCAAGGTGGGTAAATCTTGCTACTGTTAACGCGCGAGCTAAGTCAGTAGAATGGCGGGTTT
TAATAAATGAGGGAAATTGT

Downstream 100 bases:

>100_bases
GCGCTTTTAGTTGGCACCAGGTTTCAGCTGCCGCCAAGGGGCTTAGCCTGAAGGTTGGGTCCTTCGGCAGTCATTTCCAA
TACGCTGCTTTGGCTGTACC

Product: tRNA-hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MIEQLLAPVRGFLACETPDAWIEKAKDPGVLQSILVDHCNCELKAAQTALFLIRKYAVDPEYSSALLAWAKPYEEFVYHK
DRDMKAFLARTGKKNELATELKPKSGVAFGDELIARMVRLIKEEFHHFEQVLEIMHARDIPYTNINAGRYAKGMMSHVRT
SEPATLVDKLIVGAFIEARSCERFAKLAPFMDEELERFYVSLLRSEARHYQDYLTLAEMIAGENIDQRIAYFAGVEAALI
QGADDEFRFHSGVPNT

Sequences:

>Translated_256_residues
MIEQLLAPVRGFLACETPDAWIEKAKDPGVLQSILVDHCNCELKAAQTALFLIRKYAVDPEYSSALLAWAKPYEEFVYHK
DRDMKAFLARTGKKNELATELKPKSGVAFGDELIARMVRLIKEEFHHFEQVLEIMHARDIPYTNINAGRYAKGMMSHVRT
SEPATLVDKLIVGAFIEARSCERFAKLAPFMDEELERFYVSLLRSEARHYQDYLTLAEMIAGENIDQRIAYFAGVEAALI
QGADDEFRFHSGVPNT
>Mature_256_residues
MIEQLLAPVRGFLACETPDAWIEKAKDPGVLQSILVDHCNCELKAAQTALFLIRKYAVDPEYSSALLAWAKPYEEFVYHK
DRDMKAFLARTGKKNELATELKPKSGVAFGDELIARMVRLIKEEFHHFEQVLEIMHARDIPYTNINAGRYAKGMMSHVRT
SEPATLVDKLIVGAFIEARSCERFAKLAPFMDEELERFYVSLLRSEARHYQDYLTLAEMIAGENIDQRIAYFAGVEAALI
QGADDEFRFHSGVPNT

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29110; Mature: 29110

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQLLAPVRGFLACETPDAWIEKAKDPGVLQSILVDHCNCELKAAQTALFLIRKYAVDP
CHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EYSSALLAWAKPYEEFVYHKDRDMKAFLARTGKKNELATELKPKSGVAFGDELIARMVRL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
IKEEFHHFEQVLEIMHARDIPYTNINAGRYAKGMMSHVRTSEPATLVDKLIVGAFIEARS
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
CERFAKLAPFMDEELERFYVSLLRSEARHYQDYLTLAEMIAGENIDQRIAYFAGVEAALI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QGADDEFRFHSGVPNT
CCCCCHHHCCCCCCCC
>Mature Secondary Structure
MIEQLLAPVRGFLACETPDAWIEKAKDPGVLQSILVDHCNCELKAAQTALFLIRKYAVDP
CHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
EYSSALLAWAKPYEEFVYHKDRDMKAFLARTGKKNELATELKPKSGVAFGDELIARMVRL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
IKEEFHHFEQVLEIMHARDIPYTNINAGRYAKGMMSHVRTSEPATLVDKLIVGAFIEARS
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
CERFAKLAPFMDEELERFYVSLLRSEARHYQDYLTLAEMIAGENIDQRIAYFAGVEAALI
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
QGADDEFRFHSGVPNT
CCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]