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The map label for this gene is mltD [H]

Identifier: 119774323

GI number: 119774323

Start: 1450270

End: 1451466

Strand: Reverse

Name: mltD [H]

Synonym: Sama_1186

Alternate gene names: 119774323

Gene position: 1451466-1450270 (Counterclockwise)

Preceding gene: 119774329

Following gene: 119774316

Centisome position: 33.71

GC content: 56.56

Gene sequence:

>1197_bases
ATGGGAAATACAGTCATTTTAGATACTCTGCCCGGGAGGCGTCCTGCACTCAGCAAAGGCTGGTTCGTGTTGCTGCTGAC
GCTGACCATCCTTGCCGGTTGTCAGGTTAATACAGACACATCCGGGGAGCCCTGGGAGACGGACGCCCAACCTCAGCCCG
AGCAGGTCCCCCTTGTCGTCAAAGAAGCCACAGCCCCTTCCGAGCCCGCTCTGCCTGAAGCTGAAGCCAAAGCCTACCAG
AATTTATGGCAAAAAATTGCCGATGCACAGACGATAGCTGTGCCTTCCCACAGCGAAGTCACCTATTTTCTTGGGCTTAA
CCGCAATAATGGCCTCTTCCCCATTAATGCCCCCGCTGCTGCCGAGCCCTGGCTGTACGACATCATCAGTGAATTGGAAG
CAAGGGACATGCCCCTTGAGCTCGCGCTGCTGCCATTGATTGAAAGCGGCTATCATCCGGGCGTCAAGGGACTGGGGGGC
GCAGGGCTGTGGCAACTTTCCGCCCAAACAGGCCGCAATCATCACCTCAGGGTTAACCAAAGCTTCGATGGCAGGCTGGA
CCCCATTCCGGCTACCCGTGCAGCGCTGGATTATCTTCTTTACCTGTATGAGATGTTCAATAACGACTGGCTCTCAGCGG
TTGCCGCCTACAACATGGGTGAAGGTAAACTGAAAACGGCCATCGAGCGCAATCGCCGCAAGGGCAAGGCGGAGGACTTT
CTGGCGCTGGGCTTGAGCCGCAATCAGGCGCCCACCCTGTACAAATGGTTGGCGGCGCTGGCGGTTTTACGGGACCCACA
AACTCAATCAGCCAGTTTCCCGCCCATTGCCAACGCGCCTGTCTTGGCAAGGGAAGCGGTGCCCAACGGCGTGGCCCTGT
CCAATATTGCCCAGGCCATGGGTATGTCAGCCAAGGAACTGTCCAGACTGAATCCAGCCTTCAGGGACGGCATTGTGCCT
TTCAGCGGCAAATATCAAATCAACCTGCCAGAATCCCGGCTGCCCGCCCTTAACGCAGCACTGGTGAACTTAAGGCCGTC
TACCCCTGGCAAGGGCGGCAGTTATCAGGTGAAATCAGGGGATACCCTGAGTGGCATCGCCAAGCGCCATGGAATGCGTC
TGGACAAGCTGTTGTCTCTGAATGGACTCAATATGAAGAGCATTATCAAACCCGGGCAACAGTTGCGGCTGGAGTAA

Upstream 100 bases:

>100_bases
CGGCGAAAATATTTATTCATTCCCCCGATGCCAAGGGGAAATAACAGGCGTATGATTTAGCGCTTTTCGCGCCACAGGGC
AGACAGGGAGTGGAATTATC

Downstream 100 bases:

>100_bases
AGATGGGGCTGGCATAAGCCGGATTTGCGCCTTACCGGGCTTAAAATACATTTGAAGTCAGTTTGAGCAACTGTGTCAGC
CCCGAGACAAATCGTCCTGG

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MGNTVILDTLPGRRPALSKGWFVLLLTLTILAGCQVNTDTSGEPWETDAQPQPEQVPLVVKEATAPSEPALPEAEAKAYQ
NLWQKIADAQTIAVPSHSEVTYFLGLNRNNGLFPINAPAAAEPWLYDIISELEARDMPLELALLPLIESGYHPGVKGLGG
AGLWQLSAQTGRNHHLRVNQSFDGRLDPIPATRAALDYLLYLYEMFNNDWLSAVAAYNMGEGKLKTAIERNRRKGKAEDF
LALGLSRNQAPTLYKWLAALAVLRDPQTQSASFPPIANAPVLAREAVPNGVALSNIAQAMGMSAKELSRLNPAFRDGIVP
FSGKYQINLPESRLPALNAALVNLRPSTPGKGGSYQVKSGDTLSGIAKRHGMRLDKLLSLNGLNMKSIIKPGQQLRLE

Sequences:

>Translated_398_residues
MGNTVILDTLPGRRPALSKGWFVLLLTLTILAGCQVNTDTSGEPWETDAQPQPEQVPLVVKEATAPSEPALPEAEAKAYQ
NLWQKIADAQTIAVPSHSEVTYFLGLNRNNGLFPINAPAAAEPWLYDIISELEARDMPLELALLPLIESGYHPGVKGLGG
AGLWQLSAQTGRNHHLRVNQSFDGRLDPIPATRAALDYLLYLYEMFNNDWLSAVAAYNMGEGKLKTAIERNRRKGKAEDF
LALGLSRNQAPTLYKWLAALAVLRDPQTQSASFPPIANAPVLAREAVPNGVALSNIAQAMGMSAKELSRLNPAFRDGIVP
FSGKYQINLPESRLPALNAALVNLRPSTPGKGGSYQVKSGDTLSGIAKRHGMRLDKLLSLNGLNMKSIIKPGQQLRLE
>Mature_397_residues
GNTVILDTLPGRRPALSKGWFVLLLTLTILAGCQVNTDTSGEPWETDAQPQPEQVPLVVKEATAPSEPALPEAEAKAYQN
LWQKIADAQTIAVPSHSEVTYFLGLNRNNGLFPINAPAAAEPWLYDIISELEARDMPLELALLPLIESGYHPGVKGLGGA
GLWQLSAQTGRNHHLRVNQSFDGRLDPIPATRAALDYLLYLYEMFNNDWLSAVAAYNMGEGKLKTAIERNRRKGKAEDFL
ALGLSRNQAPTLYKWLAALAVLRDPQTQSASFPPIANAPVLAREAVPNGVALSNIAQAMGMSAKELSRLNPAFRDGIVPF
SGKYQINLPESRLPALNAALVNLRPSTPGKGGSYQVKSGDTLSGIAKRHGMRLDKLLSLNGLNMKSIIKPGQQLRLE

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=386, Percent_Identity=29.5336787564767, Blast_Score=172, Evalue=4e-44,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 43209; Mature: 43078

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNTVILDTLPGRRPALSKGWFVLLLTLTILAGCQVNTDTSGEPWETDAQPQPEQVPLVV
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEE
KEATAPSEPALPEAEAKAYQNLWQKIADAQTIAVPSHSEVTYFLGLNRNNGLFPINAPAA
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEECCCCCC
AEPWLYDIISELEARDMPLELALLPLIESGYHPGVKGLGGAGLWQLSAQTGRNHHLRVNQ
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECC
SFDGRLDPIPATRAALDYLLYLYEMFNNDWLSAVAAYNMGEGKLKTAIERNRRKGKAEDF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
LALGLSRNQAPTLYKWLAALAVLRDPQTQSASFPPIANAPVLAREAVPNGVALSNIAQAM
HEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
GMSAKELSRLNPAFRDGIVPFSGKYQINLPESRLPALNAALVNLRPSTPGKGGSYQVKSG
CCCHHHHHHCCHHHHCCCCCCCCEEEEECCHHHCCHHHEEEEEECCCCCCCCCEEEECCC
DTLSGIAKRHGMRLDKLLSLNGLNMKSIIKPGQQLRLE
CHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHCCCC
>Mature Secondary Structure 
GNTVILDTLPGRRPALSKGWFVLLLTLTILAGCQVNTDTSGEPWETDAQPQPEQVPLVV
CCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCEEE
KEATAPSEPALPEAEAKAYQNLWQKIADAQTIAVPSHSEVTYFLGLNRNNGLFPINAPAA
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCEEECCCCCC
AEPWLYDIISELEARDMPLELALLPLIESGYHPGVKGLGGAGLWQLSAQTGRNHHLRVNQ
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEEEECC
SFDGRLDPIPATRAALDYLLYLYEMFNNDWLSAVAAYNMGEGKLKTAIERNRRKGKAEDF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHH
LALGLSRNQAPTLYKWLAALAVLRDPQTQSASFPPIANAPVLAREAVPNGVALSNIAQAM
HEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
GMSAKELSRLNPAFRDGIVPFSGKYQINLPESRLPALNAALVNLRPSTPGKGGSYQVKSG
CCCHHHHHHCCHHHHCCCCCCCCEEEEECCHHHCCHHHEEEEEECCCCCCCCCEEEECCC
DTLSGIAKRHGMRLDKLLSLNGLNMKSIIKPGQQLRLE
CHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]